Ralstonia phage Gerry

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Cimandefvirus; Ralstonia virus Gerry

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5BA60|A0A7G5BA60_9CAUD Peptidase S74 domain-containing protein OS=Ralstonia phage Gerry OX=2759727 GN=23F_00021 PE=4 SV=1
MM1 pKa = 7.53LGIVQKK7 pKa = 9.44TATEE11 pKa = 3.89QLDD14 pKa = 3.56YY15 pKa = 11.26DD16 pKa = 3.64IDD18 pKa = 3.85FARR21 pKa = 11.84WMPDD25 pKa = 2.52GDD27 pKa = 4.01VLQGAGVAITPDD39 pKa = 4.4DD40 pKa = 3.84GTLTSPAYY48 pKa = 9.76EE49 pKa = 3.73IDD51 pKa = 3.6GTVVKK56 pKa = 10.55VWLAGGTAGASYY68 pKa = 11.0NVDD71 pKa = 3.27VTVATAAGRR80 pKa = 11.84IKK82 pKa = 9.03EE83 pKa = 4.4TCFKK87 pKa = 10.58IRR89 pKa = 11.84VRR91 pKa = 11.84SCC93 pKa = 2.82

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5BA69|A0A7G5BA69_9CAUD Muramidase domain-containing protein OS=Ralstonia phage Gerry OX=2759727 GN=23F_00030 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.77GEE4 pKa = 4.33GNMEE8 pKa = 4.25GEE10 pKa = 4.81GNGCAGVAPSADD22 pKa = 3.21RR23 pKa = 11.84PQRR26 pKa = 11.84TEE28 pKa = 3.87KK29 pKa = 10.52PVEE32 pKa = 4.0LLPAKK37 pKa = 7.42PTKK40 pKa = 10.63ASTTKK45 pKa = 10.49PPAPTSAIWDD55 pKa = 4.03AYY57 pKa = 8.98AAAYY61 pKa = 7.62RR62 pKa = 11.84QRR64 pKa = 11.84YY65 pKa = 7.07GAEE68 pKa = 3.75PVRR71 pKa = 11.84NAKK74 pKa = 10.27VNGQLAQLLTRR85 pKa = 11.84LGADD89 pKa = 3.47EE90 pKa = 4.91APQVAAFYY98 pKa = 9.61VWHH101 pKa = 6.59NNRR104 pKa = 11.84YY105 pKa = 8.55YY106 pKa = 9.15AQKK109 pKa = 9.01MHH111 pKa = 7.21AVDD114 pKa = 4.96CLLADD119 pKa = 3.74AEE121 pKa = 4.4KK122 pKa = 10.97LRR124 pKa = 11.84TEE126 pKa = 4.13WFTRR130 pKa = 11.84CATSPAVQGGEE141 pKa = 4.03DD142 pKa = 3.24VKK144 pKa = 10.27TRR146 pKa = 11.84RR147 pKa = 11.84KK148 pKa = 9.96FDD150 pKa = 3.2TGRR153 pKa = 11.84NNSPRR158 pKa = 11.84IRR160 pKa = 11.84THH162 pKa = 4.73NQQIRR167 pKa = 11.84RR168 pKa = 11.84SWKK171 pKa = 9.83SFRR174 pKa = 11.84TSPKK178 pKa = 9.18RR179 pKa = 11.84SPSATRR185 pKa = 11.84NSTAGPANTARR196 pKa = 11.84RR197 pKa = 11.84HH198 pKa = 4.64TSSLRR203 pKa = 11.84SPSWPRR209 pKa = 11.84FSSCLVMLFAMTTRR223 pKa = 11.84AQLPMTAKK231 pKa = 10.49AVLRR235 pKa = 11.84LSAGTAYY242 pKa = 9.84KK243 pKa = 10.06IHH245 pKa = 6.23AHH247 pKa = 6.09RR248 pKa = 11.84FFVLL252 pKa = 3.49

Molecular weight: 27.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

18474

32

4219

243.1

26.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.348 ± 0.467

0.801 ± 0.212

5.992 ± 0.208

6.566 ± 0.306

3.091 ± 0.113

8.65 ± 0.394

1.775 ± 0.101

4.525 ± 0.274

4.807 ± 0.31

7.151 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.135

3.475 ± 0.236

4.753 ± 0.171

4.2 ± 0.218

6.918 ± 0.286

5.413 ± 0.27

5.527 ± 0.312

6.317 ± 0.193

1.434 ± 0.148

2.625 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski