Sphingobium sp. (strain NBRC 103272 / SYK-6)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4050 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2IPK6|G2IPK6_SPHSK dTTP/UTP pyrophosphatase OS=Sphingobium sp. (strain NBRC 103272 / SYK-6) OX=627192 GN=SLG_35120 PE=3 SV=1
MM1 pKa = 7.45NKK3 pKa = 10.26LIALALASGAVVAAAPALAAAPDD26 pKa = 3.99PVVDD30 pKa = 4.58CSVSGLDD37 pKa = 5.13DD38 pKa = 3.4ITPAALACAGYY49 pKa = 10.97YY50 pKa = 9.62SGNYY54 pKa = 8.58LRR56 pKa = 11.84NPQWDD61 pKa = 4.0DD62 pKa = 3.19QVLILEE68 pKa = 4.78DD69 pKa = 4.07LGLDD73 pKa = 3.27ISGQSWSYY81 pKa = 11.49FDD83 pKa = 6.11DD84 pKa = 3.09NWKK87 pKa = 10.46FDD89 pKa = 3.46VSGNSITFGSGGIPTISGMTWIGLHH114 pKa = 5.88VGGGQNFPTGGQGSVFYY131 pKa = 10.83RR132 pKa = 11.84FDD134 pKa = 4.5AGDD137 pKa = 3.64GLGTLTFNDD146 pKa = 3.71YY147 pKa = 10.94GALSSLVIYY156 pKa = 8.48ATGGDD161 pKa = 3.74TPAVPEE167 pKa = 4.12PASWAMMIGGLGLAGAMLRR186 pKa = 11.84GRR188 pKa = 11.84SRR190 pKa = 11.84SAVSFSS196 pKa = 3.12

Molecular weight: 20.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2IIN4|G2IIN4_SPHSK Smr domain-containing protein OS=Sphingobium sp. (strain NBRC 103272 / SYK-6) OX=627192 GN=SLG_00390 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.05RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.4GFRR19 pKa = 11.84LRR21 pKa = 11.84MATAAGRR28 pKa = 11.84KK29 pKa = 8.87LLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.21KK41 pKa = 9.68LTAA44 pKa = 4.27

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4050

0

4050

1285391

29

5630

317.4

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.253 ± 0.061

0.82 ± 0.013

5.864 ± 0.032

5.392 ± 0.042

3.534 ± 0.026

8.996 ± 0.062

2.026 ± 0.021

5.055 ± 0.027

2.618 ± 0.03

10.079 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.542 ± 0.022

2.461 ± 0.028

5.509 ± 0.038

3.136 ± 0.022

7.675 ± 0.05

5.314 ± 0.039

5.142 ± 0.039

6.938 ± 0.038

1.43 ± 0.018

2.213 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski