Prevotella sp. CAG:487

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5PGZ3|R5PGZ3_9BACT AAA_14 domain-containing protein OS=Prevotella sp. CAG:487 OX=1262928 GN=BN679_00047 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 9.33KK3 pKa = 8.57TGVFYY8 pKa = 10.74GSSTGTCEE16 pKa = 5.32DD17 pKa = 2.96IAGRR21 pKa = 11.84IAVRR25 pKa = 11.84LGVDD29 pKa = 2.93SADD32 pKa = 3.47VHH34 pKa = 6.38NVADD38 pKa = 4.45LTADD42 pKa = 4.04MIAGYY47 pKa = 10.2DD48 pKa = 3.66VLVLGSSTWGCGEE61 pKa = 5.05LQDD64 pKa = 4.48DD65 pKa = 4.51WYY67 pKa = 10.87DD68 pKa = 3.51ALEE71 pKa = 4.2LLRR74 pKa = 11.84KK75 pKa = 8.34TGLQGKK81 pKa = 6.47TVALFGCGDD90 pKa = 3.7SEE92 pKa = 5.35SYY94 pKa = 11.7GDD96 pKa = 4.02TFCDD100 pKa = 3.28ALGALYY106 pKa = 10.39EE107 pKa = 4.11GLKK110 pKa = 10.97DD111 pKa = 3.93SGCTFAGGGVSVDD124 pKa = 3.25GYY126 pKa = 8.96TFEE129 pKa = 5.01SSTAVVDD136 pKa = 4.08GCFVGLAIDD145 pKa = 4.88DD146 pKa = 4.21VNEE149 pKa = 4.02SDD151 pKa = 4.29LTDD154 pKa = 3.46GRR156 pKa = 11.84IGAWAEE162 pKa = 3.77AVKK165 pKa = 11.11AEE167 pKa = 4.19IGG169 pKa = 3.47

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5PPB1|R5PPB1_9BACT Uncharacterized protein OS=Prevotella sp. CAG:487 OX=1262928 GN=BN679_00426 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2507

0

2507

783767

29

2535

312.6

35.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.454 ± 0.049

1.444 ± 0.02

6.122 ± 0.038

5.981 ± 0.051

4.304 ± 0.033

7.209 ± 0.048

1.986 ± 0.027

6.551 ± 0.049

5.951 ± 0.043

8.314 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.184 ± 0.024

4.859 ± 0.042

3.65 ± 0.03

2.924 ± 0.025

5.591 ± 0.042

6.36 ± 0.039

5.781 ± 0.035

6.791 ± 0.039

1.206 ± 0.023

4.326 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski