Amino acid dipepetide frequency for Methanobrevibacter cuticularis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.21AlaAla: 3.21 ± 0.111
0.649AlaCys: 0.649 ± 0.042
2.831AlaAsp: 2.831 ± 0.082
3.146AlaGlu: 3.146 ± 0.092
1.909AlaPhe: 1.909 ± 0.057
3.663AlaGly: 3.663 ± 0.109
0.825AlaHis: 0.825 ± 0.039
7.378AlaIle: 7.378 ± 0.142
4.688AlaLys: 4.688 ± 0.097
4.518AlaLeu: 4.518 ± 0.116
1.355AlaMet: 1.355 ± 0.05
3.634AlaAsn: 3.634 ± 0.094
1.538AlaPro: 1.538 ± 0.055
1.088AlaGln: 1.088 ± 0.043
1.652AlaArg: 1.652 ± 0.056
3.405AlaSer: 3.405 ± 0.091
3.295AlaThr: 3.295 ± 0.1
3.378AlaVal: 3.378 ± 0.113
0.296AlaTrp: 0.296 ± 0.025
1.718AlaTyr: 1.718 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.505CysAla: 0.505 ± 0.031
0.169CysCys: 0.169 ± 0.019
0.676CysAsp: 0.676 ± 0.035
0.742CysGlu: 0.742 ± 0.043
0.391CysPhe: 0.391 ± 0.027
1.115CysGly: 1.115 ± 0.051
0.195CysHis: 0.195 ± 0.018
0.808CysIle: 0.808 ± 0.048
0.701CysLys: 0.701 ± 0.04
0.659CysLeu: 0.659 ± 0.036
0.207CysMet: 0.207 ± 0.022
0.662CysAsn: 0.662 ± 0.034
0.686CysPro: 0.686 ± 0.053
0.21CysGln: 0.21 ± 0.018
0.295CysArg: 0.295 ± 0.026
0.732CysSer: 0.732 ± 0.047
0.573CysThr: 0.573 ± 0.039
0.634CysVal: 0.634 ± 0.042
0.059CysTrp: 0.059 ± 0.01
0.352CysTyr: 0.352 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
2.697AspAla: 2.697 ± 0.073
0.588AspCys: 0.588 ± 0.034
3.28AspAsp: 3.28 ± 0.093
4.823AspGlu: 4.823 ± 0.124
2.983AspPhe: 2.983 ± 0.084
3.547AspGly: 3.547 ± 0.109
0.788AspHis: 0.788 ± 0.039
6.255AspIle: 6.255 ± 0.121
4.713AspLys: 4.713 ± 0.106
5.463AspLeu: 5.463 ± 0.112
1.408AspMet: 1.408 ± 0.053
4.403AspAsn: 4.403 ± 0.121
1.916AspPro: 1.916 ± 0.06
0.925AspGln: 0.925 ± 0.043
1.359AspArg: 1.359 ± 0.053
3.871AspSer: 3.871 ± 0.089
2.494AspThr: 2.494 ± 0.075
3.929AspVal: 3.929 ± 0.106
0.381AspTrp: 0.381 ± 0.028
2.468AspTyr: 2.468 ± 0.067
0.0AspXaa: 0.0 ± 0.0
Glu
3.451GluAla: 3.451 ± 0.103
0.595GluCys: 0.595 ± 0.041
4.215GluAsp: 4.215 ± 0.092
5.912GluGlu: 5.912 ± 0.159
2.885GluPhe: 2.885 ± 0.086
3.742GluGly: 3.742 ± 0.093
0.955GluHis: 0.955 ± 0.044
7.544GluIle: 7.544 ± 0.158
7.017GluLys: 7.017 ± 0.156
6.133GluLeu: 6.133 ± 0.11
1.57GluMet: 1.57 ± 0.054
6.004GluAsn: 6.004 ± 0.126
1.343GluPro: 1.343 ± 0.053
1.232GluGln: 1.232 ± 0.048
2.053GluArg: 2.053 ± 0.075
3.879GluSer: 3.879 ± 0.092
3.217GluThr: 3.217 ± 0.088
3.829GluVal: 3.829 ± 0.091
0.408GluTrp: 0.408 ± 0.025
2.458GluTyr: 2.458 ± 0.065
0.0GluXaa: 0.0 ± 0.0
Phe
2.048PheAla: 2.048 ± 0.07
0.412PheCys: 0.412 ± 0.028
2.683PheAsp: 2.683 ± 0.083
2.875PheGlu: 2.875 ± 0.081
1.708PhePhe: 1.708 ± 0.077
2.631PheGly: 2.631 ± 0.079
0.673PheHis: 0.673 ± 0.037
4.157PheIle: 4.157 ± 0.124
3.832PheLys: 3.832 ± 0.099
3.818PheLeu: 3.818 ± 0.114
0.884PheMet: 0.884 ± 0.038
3.136PheAsn: 3.136 ± 0.085
1.265PhePro: 1.265 ± 0.046
0.872PheGln: 0.872 ± 0.041
1.169PheArg: 1.169 ± 0.042
3.144PheSer: 3.144 ± 0.081
2.636PheThr: 2.636 ± 0.129
2.502PheVal: 2.502 ± 0.082
0.229PheTrp: 0.229 ± 0.021
1.55PheTyr: 1.55 ± 0.06
0.0PheXaa: 0.0 ± 0.0
Gly
4.53GlyAla: 4.53 ± 0.164
0.728GlyCys: 0.728 ± 0.039
3.647GlyAsp: 3.647 ± 0.091
3.807GlyGlu: 3.807 ± 0.103
2.849GlyPhe: 2.849 ± 0.086
4.981GlyGly: 4.981 ± 0.186
1.035GlyHis: 1.035 ± 0.048
6.98GlyIle: 6.98 ± 0.162
5.089GlyLys: 5.089 ± 0.1
5.057GlyLeu: 5.057 ± 0.115
1.442GlyMet: 1.442 ± 0.054
4.444GlyAsn: 4.444 ± 0.156
1.474GlyPro: 1.474 ± 0.061
1.32GlyGln: 1.32 ± 0.05
2.001GlyArg: 2.001 ± 0.064
4.61GlySer: 4.61 ± 0.114
3.925GlyThr: 3.925 ± 0.128
4.499GlyVal: 4.499 ± 0.12
0.424GlyTrp: 0.424 ± 0.028
2.328GlyTyr: 2.328 ± 0.075
0.0GlyXaa: 0.0 ± 0.0
His
0.72HisAla: 0.72 ± 0.035
0.23HisCys: 0.23 ± 0.02
0.989HisAsp: 0.989 ± 0.037
1.081HisGlu: 1.081 ± 0.047
0.651HisPhe: 0.651 ± 0.036
1.135HisGly: 1.135 ± 0.045
0.327HisHis: 0.327 ± 0.024
1.323HisIle: 1.323 ± 0.051
1.049HisLys: 1.049 ± 0.04
1.35HisLeu: 1.35 ± 0.049
0.364HisMet: 0.364 ± 0.025
0.913HisAsn: 0.913 ± 0.045
0.734HisPro: 0.734 ± 0.036
0.385HisGln: 0.385 ± 0.028
0.527HisArg: 0.527 ± 0.028
0.937HisSer: 0.937 ± 0.043
0.766HisThr: 0.766 ± 0.042
0.876HisVal: 0.876 ± 0.043
0.105HisTrp: 0.105 ± 0.013
0.59HisTyr: 0.59 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
7.217IleAla: 7.217 ± 0.141
1.003IleCys: 1.003 ± 0.053
6.239IleAsp: 6.239 ± 0.12
7.086IleGlu: 7.086 ± 0.138
4.3IlePhe: 4.3 ± 0.122
6.509IleGly: 6.509 ± 0.122
1.647IleHis: 1.647 ± 0.055
10.085IleIle: 10.085 ± 0.202
8.316IleLys: 8.316 ± 0.142
8.76IleLeu: 8.76 ± 0.166
1.95IleMet: 1.95 ± 0.064
7.644IleAsn: 7.644 ± 0.165
3.705IlePro: 3.705 ± 0.089
1.979IleGln: 1.979 ± 0.062
2.895IleArg: 2.895 ± 0.074
7.503IleSer: 7.503 ± 0.127
6.155IleThr: 6.155 ± 0.175
6.117IleVal: 6.117 ± 0.129
0.507IleTrp: 0.507 ± 0.034
3.536IleTyr: 3.536 ± 0.099
0.0IleXaa: 0.0 ± 0.0
Lys
4.162LysAla: 4.162 ± 0.107
0.822LysCys: 0.822 ± 0.052
5.07LysAsp: 5.07 ± 0.106
7.23LysGlu: 7.23 ± 0.148
3.076LysPhe: 3.076 ± 0.078
4.442LysGly: 4.442 ± 0.099
1.264LysHis: 1.264 ± 0.058
9.702LysIle: 9.702 ± 0.15
8.83LysLys: 8.83 ± 0.192
7.151LysLeu: 7.151 ± 0.15
1.992LysMet: 1.992 ± 0.063
7.64LysAsn: 7.64 ± 0.151
2.072LysPro: 2.072 ± 0.063
1.823LysGln: 1.823 ± 0.061
2.816LysArg: 2.816 ± 0.077
5.242LysSer: 5.242 ± 0.124
4.82LysThr: 4.82 ± 0.104
4.552LysVal: 4.552 ± 0.094
0.51LysTrp: 0.51 ± 0.033
3.664LysTyr: 3.664 ± 0.095
0.0LysXaa: 0.0 ± 0.0
Leu
5.196LeuAla: 5.196 ± 0.113
0.749LeuCys: 0.749 ± 0.038
5.336LeuAsp: 5.336 ± 0.113
6.07LeuGlu: 6.07 ± 0.11
3.719LeuPhe: 3.719 ± 0.103
5.302LeuGly: 5.302 ± 0.118
1.083LeuHis: 1.083 ± 0.045
8.12LeuIle: 8.12 ± 0.155
8.367LeuLys: 8.367 ± 0.164
7.373LeuLeu: 7.373 ± 0.187
1.952LeuMet: 1.952 ± 0.061
6.478LeuAsn: 6.478 ± 0.131
2.783LeuPro: 2.783 ± 0.116
1.587LeuGln: 1.587 ± 0.05
2.555LeuArg: 2.555 ± 0.068
6.117LeuSer: 6.117 ± 0.1
4.589LeuThr: 4.589 ± 0.089
4.947LeuVal: 4.947 ± 0.113
0.456LeuTrp: 0.456 ± 0.028
2.392LeuTyr: 2.392 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
1.306MetAla: 1.306 ± 0.053
0.213MetCys: 0.213 ± 0.018
1.494MetAsp: 1.494 ± 0.057
1.613MetGlu: 1.613 ± 0.051
0.871MetPhe: 0.871 ± 0.045
1.557MetGly: 1.557 ± 0.057
0.327MetHis: 0.327 ± 0.021
2.138MetIle: 2.138 ± 0.056
2.219MetLys: 2.219 ± 0.069
1.559MetLeu: 1.559 ± 0.058
0.498MetMet: 0.498 ± 0.03
1.587MetAsn: 1.587 ± 0.059
0.681MetPro: 0.681 ± 0.035
0.43MetGln: 0.43 ± 0.028
0.703MetArg: 0.703 ± 0.035
1.482MetSer: 1.482 ± 0.053
1.028MetThr: 1.028 ± 0.044
1.445MetVal: 1.445 ± 0.045
0.127MetTrp: 0.127 ± 0.014
0.578MetTyr: 0.578 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.082AsnAla: 3.082 ± 0.091
0.905AsnCys: 0.905 ± 0.054
3.89AsnAsp: 3.89 ± 0.099
4.549AsnGlu: 4.549 ± 0.096
3.137AsnPhe: 3.137 ± 0.121
4.906AsnGly: 4.906 ± 0.186
1.098AsnHis: 1.098 ± 0.043
8.503AsnIle: 8.503 ± 0.169
6.654AsnLys: 6.654 ± 0.153
6.268AsnLeu: 6.268 ± 0.107
1.582AsnMet: 1.582 ± 0.057
8.935AsnAsn: 8.935 ± 0.415
2.678AsnPro: 2.678 ± 0.074
1.808AsnGln: 1.808 ± 0.054
2.06AsnArg: 2.06 ± 0.064
6.416AsnSer: 6.416 ± 0.205
4.735AsnThr: 4.735 ± 0.199
4.547AsnVal: 4.547 ± 0.125
0.469AsnTrp: 0.469 ± 0.029
3.324AsnTyr: 3.324 ± 0.108
0.0AsnXaa: 0.0 ± 0.0
Pro
1.421ProAla: 1.421 ± 0.058
0.356ProCys: 0.356 ± 0.024
1.635ProAsp: 1.635 ± 0.058
2.387ProGlu: 2.387 ± 0.066
1.393ProPhe: 1.393 ± 0.052
1.794ProGly: 1.794 ± 0.072
0.568ProHis: 0.568 ± 0.034
2.988ProIle: 2.988 ± 0.083
2.543ProLys: 2.543 ± 0.069
2.717ProLeu: 2.717 ± 0.081
0.647ProMet: 0.647 ± 0.034
2.097ProAsn: 2.097 ± 0.067
0.94ProPro: 0.94 ± 0.054
0.747ProGln: 0.747 ± 0.033
0.874ProArg: 0.874 ± 0.043
2.104ProSer: 2.104 ± 0.07
1.809ProThr: 1.809 ± 0.079
2.119ProVal: 2.119 ± 0.067
0.197ProTrp: 0.197 ± 0.019
1.123ProTyr: 1.123 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
1.081GlnAla: 1.081 ± 0.049
0.186GlnCys: 0.186 ± 0.017
1.111GlnAsp: 1.111 ± 0.043
1.425GlnGlu: 1.425 ± 0.053
0.779GlnPhe: 0.779 ± 0.038
1.343GlnGly: 1.343 ± 0.049
0.33GlnHis: 0.33 ± 0.024
2.133GlnIle: 2.133 ± 0.057
1.979GlnLys: 1.979 ± 0.062
1.76GlnLeu: 1.76 ± 0.061
0.549GlnMet: 0.549 ± 0.027
1.542GlnAsn: 1.542 ± 0.07
0.454GlnPro: 0.454 ± 0.027
0.523GlnGln: 0.523 ± 0.032
0.739GlnArg: 0.739 ± 0.035
1.249GlnSer: 1.249 ± 0.05
1.103GlnThr: 1.103 ± 0.046
1.106GlnVal: 1.106 ± 0.042
0.205GlnTrp: 0.205 ± 0.019
0.878GlnTyr: 0.878 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
1.631ArgAla: 1.631 ± 0.053
0.337ArgCys: 0.337 ± 0.028
1.664ArgAsp: 1.664 ± 0.057
2.309ArgGlu: 2.309 ± 0.068
1.262ArgPhe: 1.262 ± 0.047
1.884ArgGly: 1.884 ± 0.063
0.456ArgHis: 0.456 ± 0.028
2.99ArgIle: 2.99 ± 0.086
2.731ArgLys: 2.731 ± 0.071
2.47ArgLeu: 2.47 ± 0.071
0.818ArgMet: 0.818 ± 0.041
1.904ArgAsn: 1.904 ± 0.064
0.903ArgPro: 0.903 ± 0.045
0.617ArgGln: 0.617 ± 0.032
1.264ArgArg: 1.264 ± 0.059
1.689ArgSer: 1.689 ± 0.056
1.628ArgThr: 1.628 ± 0.059
1.719ArgVal: 1.719 ± 0.069
0.259ArgTrp: 0.259 ± 0.02
1.064ArgTyr: 1.064 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
3.38SerAla: 3.38 ± 0.091
0.691SerCys: 0.691 ± 0.049
3.639SerAsp: 3.639 ± 0.077
3.907SerGlu: 3.907 ± 0.095
3.192SerPhe: 3.192 ± 0.079
4.918SerGly: 4.918 ± 0.157
1.15SerHis: 1.15 ± 0.046
6.383SerIle: 6.383 ± 0.118
5.943SerLys: 5.943 ± 0.116
6.114SerLeu: 6.114 ± 0.103
1.438SerMet: 1.438 ± 0.053
5.723SerAsn: 5.723 ± 0.162
2.111SerPro: 2.111 ± 0.074
1.653SerGln: 1.653 ± 0.057
1.989SerArg: 1.989 ± 0.069
5.623SerSer: 5.623 ± 0.146
4.201SerThr: 4.201 ± 0.134
3.986SerVal: 3.986 ± 0.091
0.447SerTrp: 0.447 ± 0.029
2.822SerTyr: 2.822 ± 0.075
0.0SerXaa: 0.0 ± 0.0
Thr
3.115ThrAla: 3.115 ± 0.103
0.535ThrCys: 0.535 ± 0.033
3.005ThrAsp: 3.005 ± 0.088
2.853ThrGlu: 2.853 ± 0.079
2.685ThrPhe: 2.685 ± 0.109
4.416ThrGly: 4.416 ± 0.144
0.85ThrHis: 0.85 ± 0.038
6.224ThrIle: 6.224 ± 0.18
3.983ThrLys: 3.983 ± 0.082
4.718ThrLeu: 4.718 ± 0.102
0.988ThrMet: 0.988 ± 0.038
4.781ThrAsn: 4.781 ± 0.243
1.882ThrPro: 1.882 ± 0.07
1.164ThrGln: 1.164 ± 0.049
1.623ThrArg: 1.623 ± 0.063
3.939ThrSer: 3.939 ± 0.128
3.651ThrThr: 3.651 ± 0.174
3.749ThrVal: 3.749 ± 0.133
0.305ThrTrp: 0.305 ± 0.021
1.862ThrTyr: 1.862 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
3.481ValAla: 3.481 ± 0.094
0.644ValCys: 0.644 ± 0.035
4.274ValAsp: 4.274 ± 0.111
3.834ValGlu: 3.834 ± 0.083
2.455ValPhe: 2.455 ± 0.072
3.932ValGly: 3.932 ± 0.112
0.781ValHis: 0.781 ± 0.041
5.643ValIle: 5.643 ± 0.118
4.733ValLys: 4.733 ± 0.101
5.26ValLeu: 5.26 ± 0.095
1.323ValMet: 1.323 ± 0.052
4.467ValAsn: 4.467 ± 0.156
1.953ValPro: 1.953 ± 0.059
1.164ValGln: 1.164 ± 0.043
1.694ValArg: 1.694 ± 0.063
4.439ValSer: 4.439 ± 0.121
3.493ValThr: 3.493 ± 0.117
4.218ValVal: 4.218 ± 0.113
0.337ValTrp: 0.337 ± 0.028
2.316ValTyr: 2.316 ± 0.083
0.0ValXaa: 0.0 ± 0.0
Trp
0.286TrpAla: 0.286 ± 0.023
0.083TrpCys: 0.083 ± 0.013
0.388TrpAsp: 0.388 ± 0.028
0.479TrpGlu: 0.479 ± 0.029
0.256TrpPhe: 0.256 ± 0.025
0.452TrpGly: 0.452 ± 0.026
0.095TrpHis: 0.095 ± 0.012
0.591TrpIle: 0.591 ± 0.029
0.523TrpLys: 0.523 ± 0.031
0.503TrpLeu: 0.503 ± 0.028
0.159TrpMet: 0.159 ± 0.017
0.429TrpAsn: 0.429 ± 0.03
0.132TrpPro: 0.132 ± 0.015
0.158TrpGln: 0.158 ± 0.018
0.207TrpArg: 0.207 ± 0.021
0.376TrpSer: 0.376 ± 0.025
0.305TrpThr: 0.305 ± 0.024
0.39TrpVal: 0.39 ± 0.027
0.114TrpTrp: 0.114 ± 0.013
0.249TrpTyr: 0.249 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.655TyrAla: 1.655 ± 0.055
0.476TyrCys: 0.476 ± 0.031
2.223TyrAsp: 2.223 ± 0.073
2.318TyrGlu: 2.318 ± 0.073
1.725TyrPhe: 1.725 ± 0.059
2.899TyrGly: 2.899 ± 0.087
0.605TyrHis: 0.605 ± 0.034
3.207TyrIle: 3.207 ± 0.08
2.97TyrLys: 2.97 ± 0.094
3.519TyrLeu: 3.519 ± 0.09
0.728TyrMet: 0.728 ± 0.036
3.122TyrAsn: 3.122 ± 0.103
1.208TyrPro: 1.208 ± 0.048
0.791TyrGln: 0.791 ± 0.037
1.127TyrArg: 1.127 ± 0.048
2.555TyrSer: 2.555 ± 0.071
1.906TyrThr: 1.906 ± 0.078
1.899TyrVal: 1.899 ± 0.068
0.341TyrTrp: 0.341 ± 0.027
1.616TyrTyr: 1.616 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2061 proteins (590290 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski