Gammaproteobacteria bacterium SCGC AG-212-F23

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177QMV9|A0A177QMV9_9GAMM Indole-3-glycerol phosphate synthase OS=Gammaproteobacteria bacterium SCGC AG-212-F23 OX=1799661 GN=AYO45_05235 PE=4 SV=1
MM1 pKa = 7.37SYY3 pKa = 10.53KK4 pKa = 10.39RR5 pKa = 11.84YY6 pKa = 8.46MCLLCGYY13 pKa = 9.73IYY15 pKa = 10.4DD16 pKa = 5.21EE17 pKa = 4.86EE18 pKa = 5.11IGWPADD24 pKa = 3.86GILPGTRR31 pKa = 11.84WEE33 pKa = 4.44DD34 pKa = 3.91VPLTWQCPDD43 pKa = 3.67CGASKK48 pKa = 11.11DD49 pKa = 3.88DD50 pKa = 4.21FEE52 pKa = 5.1MIEE55 pKa = 3.87II56 pKa = 4.4

Molecular weight: 6.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177QPP2|A0A177QPP2_9GAMM Uncharacterized protein OS=Gammaproteobacteria bacterium SCGC AG-212-F23 OX=1799661 GN=AYO45_04105 PE=4 SV=1
MM1 pKa = 6.94TRR3 pKa = 11.84KK4 pKa = 9.67LLSLGVFLFIFPALAMAGWQVAQPTASIPNEE35 pKa = 3.77PFFQTGNQRR44 pKa = 11.84DD45 pKa = 3.96KK46 pKa = 11.45TFVPPPAFHH55 pKa = 7.04GSVFPNRR62 pKa = 11.84YY63 pKa = 9.8AKK65 pKa = 9.43MGRR68 pKa = 11.84PAGVYY73 pKa = 9.88AISISGSLKK82 pKa = 10.45EE83 pKa = 3.86NLEE86 pKa = 4.67RR87 pKa = 11.84IMHH90 pKa = 6.69RR91 pKa = 11.84YY92 pKa = 7.49RR93 pKa = 11.84WRR95 pKa = 11.84VAWKK99 pKa = 10.34APYY102 pKa = 9.93DD103 pKa = 3.81YY104 pKa = 11.29NFDD107 pKa = 3.48GRR109 pKa = 11.84ITGSSLPNVIEE120 pKa = 4.8KK121 pKa = 10.57LLQPFPLQAVMYY133 pKa = 7.65MSNRR137 pKa = 11.84TLEE140 pKa = 3.67IRR142 pKa = 11.84PRR144 pKa = 11.84SS145 pKa = 3.55

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1263

0

1263

401252

37

2722

317.7

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.246 ± 0.069

1.132 ± 0.027

4.832 ± 0.046

5.585 ± 0.064

4.386 ± 0.044

5.849 ± 0.092

2.626 ± 0.036

7.606 ± 0.05

6.547 ± 0.075

10.377 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.416 ± 0.034

4.768 ± 0.058

4.197 ± 0.053

4.286 ± 0.049

4.333 ± 0.054

6.393 ± 0.079

5.974 ± 0.096

6.063 ± 0.056

1.095 ± 0.024

3.291 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski