Cangyuan orthoreovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus; unclassified Orthoreovirus; Pteropine orthoreovirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1ECY1|A0A0A1ECY1_9REOV Nonstructural protein OS=Cangyuan orthoreovirus OX=1388882 PE=4 SV=1
MM1 pKa = 7.06AQIRR5 pKa = 11.84GLRR8 pKa = 11.84LAEE11 pKa = 4.16SFNVPSKK18 pKa = 10.71SSSPSFITYY27 pKa = 10.63DD28 pKa = 3.5FLLSRR33 pKa = 11.84LQCASTPWTALTTSSGSDD51 pKa = 3.24VTVVRR56 pKa = 11.84LLFPLQGIQSPTLEE70 pKa = 4.31SPIPTTFSEE79 pKa = 4.25WKK81 pKa = 7.66TWMQEE86 pKa = 3.63RR87 pKa = 11.84LAVIYY92 pKa = 10.11EE93 pKa = 3.99DD94 pKa = 4.67LIRR97 pKa = 11.84RR98 pKa = 11.84YY99 pKa = 9.54PISEE103 pKa = 3.52FHH105 pKa = 6.15GRR107 pKa = 11.84HH108 pKa = 5.17VNPLICNTIVASFFSNSPYY127 pKa = 10.77SSLLDD132 pKa = 3.27HH133 pKa = 6.9VMLRR137 pKa = 11.84VSPAADD143 pKa = 2.91ILDD146 pKa = 4.01ANLLQRR152 pKa = 11.84NHH154 pKa = 6.25FWPLSNDD161 pKa = 3.82LLMMPAGKK169 pKa = 10.22KK170 pKa = 8.83YY171 pKa = 7.28WTFDD175 pKa = 3.27GYY177 pKa = 8.48QTSADD182 pKa = 3.84APCLFGKK189 pKa = 9.73NQTPYY194 pKa = 11.13ADD196 pKa = 3.63VIYY199 pKa = 10.72GRR201 pKa = 11.84DD202 pKa = 3.57LTSLLTFVDD211 pKa = 4.14SAPSQLTPLVHH222 pKa = 7.42LDD224 pKa = 3.35RR225 pKa = 11.84PSYY228 pKa = 10.09GPHH231 pKa = 5.64VLLPTEE237 pKa = 4.1NVIPAIATSSKK248 pKa = 11.18DD249 pKa = 3.38CAFWLLLEE257 pKa = 4.57SCLDD261 pKa = 3.47QLRR264 pKa = 11.84ANQSSSRR271 pKa = 11.84STPVTRR277 pKa = 11.84RR278 pKa = 11.84IHH280 pKa = 6.07SYY282 pKa = 10.25LVVRR286 pKa = 11.84SPYY289 pKa = 9.33FAFPEE294 pKa = 4.3SVEE297 pKa = 4.19YY298 pKa = 10.88ALLALNNASFCGSQLTVPVPNHH320 pKa = 5.64PTCSMVATLVSQLSDD335 pKa = 2.95SSKK338 pKa = 11.05AITVPSPKK346 pKa = 9.46PRR348 pKa = 11.84SFTLYY353 pKa = 10.82SNLKK357 pKa = 9.37FDD359 pKa = 4.62YY360 pKa = 9.32PQSLTPFISISPLTFTGTLGSASLLKK386 pKa = 9.16PTAASVRR393 pKa = 11.84WQPQFDD399 pKa = 4.7DD400 pKa = 4.15STSSPVDD407 pKa = 3.36LALNLMNSALTLPVTPDD424 pKa = 3.07YY425 pKa = 11.2GYY427 pKa = 11.33AWTGSPLFSYY437 pKa = 10.41AVSDD441 pKa = 3.51RR442 pKa = 11.84HH443 pKa = 6.27DD444 pKa = 3.48AAVPIDD450 pKa = 4.36LVPDD454 pKa = 5.05FPADD458 pKa = 3.63YY459 pKa = 10.44FSTEE463 pKa = 3.9QQDD466 pKa = 2.77ARR468 pKa = 11.84ARR470 pKa = 11.84FRR472 pKa = 11.84DD473 pKa = 3.4YY474 pKa = 11.31RR475 pKa = 11.84LIADD479 pKa = 4.44RR480 pKa = 11.84SLHH483 pKa = 6.83KK484 pKa = 10.38DD485 pKa = 3.21TANLACVSQQQDD497 pKa = 2.89ALGNRR502 pKa = 11.84FVFNGMSVAYY512 pKa = 8.74MGASGTHH519 pKa = 7.2PDD521 pKa = 4.33DD522 pKa = 3.8QPSVITPWLSGKK534 pKa = 9.52LASVFKK540 pKa = 10.19PSSIRR545 pKa = 11.84QFGWDD550 pKa = 3.3VTKK553 pKa = 10.96GVILDD558 pKa = 3.77VTHH561 pKa = 7.03SIASGDD567 pKa = 3.58FGFVYY572 pKa = 10.7SDD574 pKa = 3.33VDD576 pKa = 3.69QVQCNSDD583 pKa = 3.93DD584 pKa = 4.11LVSSTRR590 pKa = 11.84AFILQCQNLLTLVQVGGSLIVKK612 pKa = 9.79CNFPTSRR619 pKa = 11.84VLAWVYY625 pKa = 10.76TEE627 pKa = 4.14LSPWFNRR634 pKa = 11.84IIVMKK639 pKa = 10.01PLLSNNLEE647 pKa = 4.1IYY649 pKa = 10.6VGLLQKK655 pKa = 10.72LAITSPPFGPSSSVVVFLTKK675 pKa = 10.37QIRR678 pKa = 11.84RR679 pKa = 11.84YY680 pKa = 10.03SSLIEE685 pKa = 4.1ASVTLPDD692 pKa = 3.56RR693 pKa = 11.84GASVTLDD700 pKa = 3.27NSLTCLSLNFVNVTSVGSEE719 pKa = 3.79DD720 pKa = 4.16DD721 pKa = 4.57LRR723 pKa = 11.84ALACFSLLSSPSTIRR738 pKa = 11.84LSRR741 pKa = 11.84HH742 pKa = 5.48EE743 pKa = 4.3YY744 pKa = 9.77FDD746 pKa = 3.52SFRR749 pKa = 11.84TAVTSVVTPDD759 pKa = 4.1SRR761 pKa = 11.84RR762 pKa = 11.84LWSRR766 pKa = 11.84LAYY769 pKa = 9.71VPRR772 pKa = 11.84IFPSSLTVQSRR783 pKa = 11.84SIAYY787 pKa = 9.28SPPSLFKK794 pKa = 10.54TKK796 pKa = 10.15TSLWTLLSVYY806 pKa = 10.14YY807 pKa = 10.49DD808 pKa = 3.41WVLSGIPGRR817 pKa = 11.84PNLWMDD823 pKa = 4.41LGTGPEE829 pKa = 4.12CRR831 pKa = 11.84LLSKK835 pKa = 10.34ISEE838 pKa = 5.07DD839 pKa = 3.48IPVCMVDD846 pKa = 3.23VRR848 pKa = 11.84PSYY851 pKa = 11.44LPMNCWKK858 pKa = 8.6TQTDD862 pKa = 4.8YY863 pKa = 11.15IVKK866 pKa = 9.96DD867 pKa = 3.7YY868 pKa = 11.49TDD870 pKa = 3.35MQVILDD876 pKa = 3.9YY877 pKa = 11.54SPDD880 pKa = 3.64YY881 pKa = 11.2VSAILTLGAASFSSGVSLSSLVRR904 pKa = 11.84DD905 pKa = 4.37FLNSCKK911 pKa = 9.98QVGATKK917 pKa = 10.04IIFQLNSPADD927 pKa = 3.67TRR929 pKa = 11.84VDD931 pKa = 3.62SPHH934 pKa = 7.2RR935 pKa = 11.84EE936 pKa = 3.92LQIDD940 pKa = 3.65VSKK943 pKa = 11.33QMFFFPTLGRR953 pKa = 11.84KK954 pKa = 8.28EE955 pKa = 4.3PYY957 pKa = 10.36LPLTDD962 pKa = 4.77VIQLLSQTFPGATVEE977 pKa = 3.87IRR979 pKa = 11.84YY980 pKa = 9.5PEE982 pKa = 5.13DD983 pKa = 4.26DD984 pKa = 4.3YY985 pKa = 12.21SWLSNPLVNGLSIDD999 pKa = 3.37TDD1001 pKa = 4.57AISKK1005 pKa = 10.27LLVLSQFFPLFIVHH1019 pKa = 7.18ADD1021 pKa = 3.47VKK1023 pKa = 11.03SVTFSNVTSVGSEE1036 pKa = 3.71MSLTIHH1042 pKa = 6.59GFSSTSSYY1050 pKa = 11.63DD1051 pKa = 3.21VTLDD1055 pKa = 3.48GVSLLTISGAKK1066 pKa = 9.45VDD1068 pKa = 3.91SSLVSASLSVANDD1081 pKa = 3.52DD1082 pKa = 3.29AVLRR1086 pKa = 11.84FTPASAGILRR1096 pKa = 11.84VSQTAPVVLPIGSTVISAPDD1116 pKa = 3.44DD1117 pKa = 4.71AITVVWPSSLDD1128 pKa = 3.49YY1129 pKa = 11.37SDD1131 pKa = 5.5AGTNVSITCNSWFTLRR1147 pKa = 11.84LFAEE1151 pKa = 4.41RR1152 pKa = 11.84DD1153 pKa = 3.59GEE1155 pKa = 4.45VIRR1158 pKa = 11.84VSDD1161 pKa = 4.03DD1162 pKa = 3.32KK1163 pKa = 11.84YY1164 pKa = 10.99DD1165 pKa = 3.5IRR1167 pKa = 11.84PAGSNSWTLNVILDD1181 pKa = 3.96RR1182 pKa = 11.84SDD1184 pKa = 2.74AFYY1187 pKa = 11.06RR1188 pKa = 11.84FFLRR1192 pKa = 11.84DD1193 pKa = 3.34VQSAVPGQYY1202 pKa = 9.51IDD1204 pKa = 6.01FEE1206 pKa = 4.36LQQLSIHH1213 pKa = 5.68VWDD1216 pKa = 4.26PSKK1219 pKa = 10.84PSFLSPPYY1227 pKa = 10.41NGDD1230 pKa = 3.62FVISTPGGPVQLSRR1244 pKa = 11.84PFTQIPADD1252 pKa = 3.32WKK1254 pKa = 8.01TVNVSVSVISDD1265 pKa = 3.92LPSFLVPPSEE1275 pKa = 4.52YY1276 pKa = 10.71YY1277 pKa = 11.0GIVPVV1282 pKa = 3.94

Molecular weight: 141.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1EAH6|A0A0A1EAH6_9REOV Nonstructural protein OS=Cangyuan orthoreovirus OX=1388882 PE=4 SV=1
MM1 pKa = 7.96AYY3 pKa = 10.33LALPVPEE10 pKa = 5.49GPDD13 pKa = 3.4SQSALISAVNGYY25 pKa = 9.95RR26 pKa = 11.84PDD28 pKa = 3.38GVAFDD33 pKa = 4.82DD34 pKa = 4.28VHH36 pKa = 8.29VLDD39 pKa = 4.19TKK41 pKa = 10.74RR42 pKa = 11.84VLRR45 pKa = 11.84QFDD48 pKa = 3.95LMTTGIRR55 pKa = 11.84ADD57 pKa = 3.89DD58 pKa = 3.74VAKK61 pKa = 10.85GLLLRR66 pKa = 11.84DD67 pKa = 3.44WRR69 pKa = 11.84RR70 pKa = 11.84QSVFVLLPQKK80 pKa = 9.82TSLLEE85 pKa = 4.09YY86 pKa = 10.73LLTAPKK92 pKa = 10.1TSLPDD97 pKa = 3.44GLTPQLLRR105 pKa = 11.84KK106 pKa = 9.98FKK108 pKa = 10.97AKK110 pKa = 10.18PHH112 pKa = 6.15DD113 pKa = 4.02FKK115 pKa = 11.58YY116 pKa = 10.98SDD118 pKa = 3.31WFSPLVTEE126 pKa = 4.93HH127 pKa = 6.69SSVVQSVRR135 pKa = 11.84YY136 pKa = 9.4LNSHH140 pKa = 6.08PVVFTTTVKK149 pKa = 10.95VIGAPVRR156 pKa = 11.84LFAPFKK162 pKa = 10.92YY163 pKa = 10.33FDD165 pKa = 3.54ISQGILSDD173 pKa = 4.76LMILKK178 pKa = 9.28MNHH181 pKa = 5.75QLPPLPAFRR190 pKa = 11.84VMVSMFPSSASGSCVLPPIDD210 pKa = 3.53EE211 pKa = 4.09WRR213 pKa = 11.84NSNTHH218 pKa = 5.77PVCLLLASMYY228 pKa = 10.57DD229 pKa = 3.39SSYY232 pKa = 11.07KK233 pKa = 9.63ATARR237 pKa = 11.84YY238 pKa = 9.54LDD240 pKa = 3.76RR241 pKa = 11.84SLITAMLCGQRR252 pKa = 11.84RR253 pKa = 11.84VKK255 pKa = 9.32QVKK258 pKa = 9.62YY259 pKa = 9.82SPLMARR265 pKa = 11.84AARR268 pKa = 11.84SVGLSVNVTNPTRR281 pKa = 11.84QINTSLIDD289 pKa = 3.34VHH291 pKa = 5.88TVVIDD296 pKa = 3.61VRR298 pKa = 11.84SSLDD302 pKa = 3.25NRR304 pKa = 11.84LTPTRR309 pKa = 11.84LRR311 pKa = 11.84FCGVPVALTAHH322 pKa = 6.36MGVAPSEE329 pKa = 4.14DD330 pKa = 3.03WLAVRR335 pKa = 11.84DD336 pKa = 3.97EE337 pKa = 4.41TGMFVDD343 pKa = 4.12WFLVLTLFSDD353 pKa = 4.73RR354 pKa = 11.84IKK356 pKa = 11.14GPTGQHH362 pKa = 4.6VCLNPLSTSVDD373 pKa = 3.14SVNFVRR379 pKa = 11.84VHH381 pKa = 6.36GYY383 pKa = 9.84VSQHH387 pKa = 4.69VQSLKK392 pKa = 7.85PWQYY396 pKa = 11.22GRR398 pKa = 11.84LSSFGVAMAKK408 pKa = 10.47GSFKK412 pKa = 10.63STMTRR417 pKa = 11.84FLTSLTIAGTRR428 pKa = 11.84LIFPNVIADD437 pKa = 3.58SDD439 pKa = 4.18DD440 pKa = 4.45PGDD443 pKa = 3.76SLEE446 pKa = 4.28PTFEE450 pKa = 4.1NQVLAEE456 pKa = 4.43LSSVDD461 pKa = 3.6ADD463 pKa = 3.49WEE465 pKa = 4.49RR466 pKa = 11.84KK467 pKa = 9.13VFTASGTVDD476 pKa = 3.27TSYY479 pKa = 11.35LSTVIFPIFLRR490 pKa = 11.84LFRR493 pKa = 11.84SEE495 pKa = 4.83LSPHH499 pKa = 6.02SRR501 pKa = 11.84TFYY504 pKa = 10.91DD505 pKa = 3.56EE506 pKa = 4.32RR507 pKa = 11.84ASSARR512 pKa = 11.84TLTFAHH518 pKa = 7.12ADD520 pKa = 3.6AEE522 pKa = 4.23FLDD525 pKa = 4.5AGWVDD530 pKa = 4.3RR531 pKa = 11.84IEE533 pKa = 4.03RR534 pKa = 11.84CYY536 pKa = 10.52IHH538 pKa = 7.24YY539 pKa = 10.61DD540 pKa = 3.3EE541 pKa = 5.12EE542 pKa = 5.53RR543 pKa = 11.84NVLLRR548 pKa = 11.84SSRR551 pKa = 11.84VGGSTFQLVLSRR563 pKa = 11.84CYY565 pKa = 11.28KK566 pKa = 10.01MIASPAPSEE575 pKa = 4.03PVSMLLKK582 pKa = 10.53ALVGGWLSAGPVLSSLSHH600 pKa = 5.69SASARR605 pKa = 11.84VLAWYY610 pKa = 9.72IDD612 pKa = 3.41DD613 pKa = 4.26HH614 pKa = 7.66HH615 pKa = 6.69WVDD618 pKa = 5.69HH619 pKa = 6.05GWCLCDD625 pKa = 3.6KK626 pKa = 9.66RR627 pKa = 11.84KK628 pKa = 9.87HH629 pKa = 4.3VTFSFMRR636 pKa = 11.84GHH638 pKa = 7.28PDD640 pKa = 3.23DD641 pKa = 5.52LAVLDD646 pKa = 4.08LQDD649 pKa = 3.14WSKK652 pKa = 11.13YY653 pKa = 8.75RR654 pKa = 11.84ATISVLTDD662 pKa = 3.57PLDD665 pKa = 4.13FGSSLRR671 pKa = 11.84VVAARR676 pKa = 11.84VYY678 pKa = 7.54WTSQKK683 pKa = 10.52PSVDD687 pKa = 3.38VFDD690 pKa = 4.47NRR692 pKa = 11.84ALLTPFQTYY701 pKa = 9.35HH702 pKa = 6.07VSLNCACPLGVRR714 pKa = 11.84FQVKK718 pKa = 9.94NVGLKK723 pKa = 10.22LATVSGAA730 pKa = 3.25

Molecular weight: 81.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

7339

328

1290

733.9

80.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.094 ± 0.465

1.403 ± 0.073

5.832 ± 0.261

3.42 ± 0.458

3.992 ± 0.296

4.592 ± 0.261

1.908 ± 0.299

4.401 ± 0.356

3.311 ± 0.295

10.533 ± 0.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.739 ± 0.325

4.006 ± 0.464

6.295 ± 0.386

3.897 ± 0.264

5.409 ± 0.389

10.778 ± 0.714

7.113 ± 0.464

7.944 ± 0.331

1.676 ± 0.119

2.643 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski