Watermelon chlorotic stunt virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9DHW8|Q9DHW8_9GEMI Protein V2 OS=Watermelon chlorotic stunt virus OX=35341 GN=v2 PE=3 SV=1
MM1 pKa = 8.09WDD3 pKa = 3.51PLLNDD8 pKa = 3.92FPEE11 pKa = 4.65SVHH14 pKa = 6.55GFRR17 pKa = 11.84CMLAVKK23 pKa = 9.66YY24 pKa = 9.52LQAVEE29 pKa = 4.26STYY32 pKa = 11.06EE33 pKa = 4.06PNTLGHH39 pKa = 6.12EE40 pKa = 4.97LIRR43 pKa = 11.84DD44 pKa = 4.52LILVLRR50 pKa = 11.84ARR52 pKa = 11.84DD53 pKa = 3.47YY54 pKa = 11.53GEE56 pKa = 3.78ANRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 9.62SHH63 pKa = 5.7FHH65 pKa = 5.5SRR67 pKa = 11.84FEE69 pKa = 4.34GSSKK73 pKa = 9.98TEE75 pKa = 3.58LRR77 pKa = 11.84QPLHH81 pKa = 6.57EE82 pKa = 4.74PCCCPHH88 pKa = 7.01CPGHH92 pKa = 6.05KK93 pKa = 9.39QASTMGQQAHH103 pKa = 5.34VSKK106 pKa = 10.77AQDD109 pKa = 3.43VQDD112 pKa = 3.68VSKK115 pKa = 10.39PRR117 pKa = 11.84CPP119 pKa = 3.51

Molecular weight: 13.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9DHW7|Q9DHW7_9GEMI Capsid protein OS=Watermelon chlorotic stunt virus OX=35341 GN=coat protein PE=3 SV=1
MM1 pKa = 8.21DD2 pKa = 4.63SRR4 pKa = 11.84TGEE7 pKa = 4.49YY8 pKa = 8.57ITADD12 pKa = 3.57QAWNGVFTWKK22 pKa = 10.26INNPLYY28 pKa = 9.64FTITEE33 pKa = 3.85HH34 pKa = 5.75HH35 pKa = 6.13QRR37 pKa = 11.84PFLCRR42 pKa = 11.84HH43 pKa = 7.14DD44 pKa = 4.56IIQMQIRR51 pKa = 11.84FNHH54 pKa = 5.56NLRR57 pKa = 11.84RR58 pKa = 11.84ALGIHH63 pKa = 6.29KK64 pKa = 10.37AFLNFRR70 pKa = 11.84IWTTLRR76 pKa = 11.84PQTGRR81 pKa = 11.84FLRR84 pKa = 11.84VFRR87 pKa = 11.84TQVLKK92 pKa = 10.92YY93 pKa = 10.45LDD95 pKa = 3.79MLGVISLNLVVRR107 pKa = 11.84AVSHH111 pKa = 5.02VLEE114 pKa = 4.38NVLIGTIDD122 pKa = 3.46VQEE125 pKa = 3.99KK126 pKa = 10.38HH127 pKa = 6.48VIKK130 pKa = 10.62FDD132 pKa = 4.17LYY134 pKa = 11.03

Molecular weight: 15.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1610

47

361

201.3

23.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.907 ± 0.524

2.112 ± 0.352

5.093 ± 0.267

4.845 ± 0.604

4.596 ± 0.332

5.031 ± 0.453

3.23 ± 0.51

5.155 ± 0.718

5.714 ± 0.528

7.391 ± 0.74

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.422 ± 0.38

4.969 ± 0.332

5.714 ± 0.609

4.286 ± 0.334

7.826 ± 0.821

8.82 ± 1.131

5.963 ± 0.932

6.708 ± 1.129

1.304 ± 0.18

3.913 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski