Aquila chrysaetos chrysaetos

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A663EC83|A0A663EC83_AQUCH Mannosyltransferase OS=Aquila chrysaetos chrysaetos OX=223781 GN=PIGB PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 4.61KK3 pKa = 10.51CTDD6 pKa = 3.5TEE8 pKa = 4.09EE9 pKa = 4.46DD10 pKa = 3.55QLLEE14 pKa = 4.41EE15 pKa = 5.27PDD17 pKa = 3.17IQYY20 pKa = 10.81SSKK23 pKa = 9.05VTQHH27 pKa = 6.28PSIASSIAEE36 pKa = 4.36SKK38 pKa = 10.7SSPGSDD44 pKa = 2.91GASSPEE50 pKa = 4.06GPEE53 pKa = 4.08LVYY56 pKa = 10.66IPAKK60 pKa = 9.4PAQLAAHH67 pKa = 5.94VLGNTDD73 pKa = 2.2SFYY76 pKa = 11.09SVRR79 pKa = 11.84DD80 pKa = 3.41VATSVQTLHH89 pKa = 7.14EE90 pKa = 4.8DD91 pKa = 3.46SQTSEE96 pKa = 4.2NEE98 pKa = 4.02CTPVEE103 pKa = 4.58GAAEE107 pKa = 4.36DD108 pKa = 4.47PSAVPAADD116 pKa = 3.41ASVEE120 pKa = 4.09AVTPQPGAWSQSSIAGEE137 pKa = 4.34LGPSDD142 pKa = 3.62SQADD146 pKa = 3.61VSTNDD151 pKa = 2.67VDD153 pKa = 3.87QASLVSLWEE162 pKa = 4.64DD163 pKa = 3.76PSPLPLSLPPSPTPKK178 pKa = 10.55DD179 pKa = 3.35PLQAVPSCNEE189 pKa = 3.77AEE191 pKa = 4.43VTSTTEE197 pKa = 4.14VVSLCWDD204 pKa = 3.89DD205 pKa = 4.42EE206 pKa = 5.03LIMDD210 pKa = 5.26AGVLPYY216 pKa = 10.35VEE218 pKa = 5.19QFPQKK223 pKa = 10.38IIIPFVDD230 pKa = 3.32QTAYY234 pKa = 10.73LLKK237 pKa = 10.13IEE239 pKa = 4.26QPLNVGQGLSATASGSSDD257 pKa = 3.73DD258 pKa = 5.84DD259 pKa = 4.06LVCHH263 pKa = 6.42PEE265 pKa = 3.99RR266 pKa = 11.84TEE268 pKa = 3.76STVQVEE274 pKa = 4.41SAEE277 pKa = 4.11QVYY280 pKa = 10.64VMSGKK285 pKa = 10.05KK286 pKa = 7.88VWVFVSSSCFSFQLYY301 pKa = 10.74SFISGHH307 pKa = 5.53

Molecular weight: 32.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A663ER34|A0A663ER34_AQUCH Transmembrane protein 222 OS=Aquila chrysaetos chrysaetos OX=223781 GN=TMEM222 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 5.03PRR4 pKa = 11.84APSALTVPDD13 pKa = 4.43PPSPEE18 pKa = 4.19SPRR21 pKa = 11.84PAHH24 pKa = 6.43GPGPHH29 pKa = 6.33VSPPGPSAPSPATRR43 pKa = 11.84QQGRR47 pKa = 11.84SPAPHH52 pKa = 6.12SPARR56 pKa = 11.84PWAPRR61 pKa = 11.84GQAHH65 pKa = 7.13PWAHH69 pKa = 6.84LPPVGPSPARR79 pKa = 11.84GPIISRR85 pKa = 11.84PWAHH89 pKa = 7.05LPPVGPSPAHH99 pKa = 5.96GPIISRR105 pKa = 11.84PWAHH109 pKa = 7.05LPPVGPSPTHH119 pKa = 6.21GPISRR124 pKa = 11.84PWAHH128 pKa = 6.85HH129 pKa = 6.43LPPVGPSPARR139 pKa = 11.84GPIISRR145 pKa = 11.84PWAHH149 pKa = 6.82HH150 pKa = 6.43LPPVGPSPARR160 pKa = 11.84GPISHH165 pKa = 7.58PWAHH169 pKa = 6.95LPPVGPSSPARR180 pKa = 11.84GPISHH185 pKa = 7.58PWAHH189 pKa = 6.95LPPVGPSSPARR200 pKa = 11.84GPISRR205 pKa = 11.84PWAHH209 pKa = 6.85HH210 pKa = 6.43LPPVGPSPTRR220 pKa = 11.84GPISRR225 pKa = 11.84PWAHH229 pKa = 6.85HH230 pKa = 6.43LPPVGPSSPARR241 pKa = 11.84GPISRR246 pKa = 11.84PWAHH250 pKa = 6.85HH251 pKa = 6.43LPPVGPSPARR261 pKa = 11.84GPISHH266 pKa = 7.58PWAHH270 pKa = 6.95LPPVGPSSPARR281 pKa = 11.84GPISHH286 pKa = 7.58PWAHH290 pKa = 6.95LPPVGPSSPARR301 pKa = 11.84GPIISRR307 pKa = 11.84PWAHH311 pKa = 7.05LPPVGPSPGLAPSPRR326 pKa = 11.84RR327 pKa = 11.84CPPPVPP333 pKa = 3.91

Molecular weight: 34.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16059

8758

24817

16571618

20

9572

667.8

74.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.905 ± 0.014

2.234 ± 0.014

4.985 ± 0.009

7.268 ± 0.02

3.623 ± 0.01

6.226 ± 0.02

2.514 ± 0.007

4.648 ± 0.011

6.101 ± 0.019

9.672 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.006

3.924 ± 0.01

5.715 ± 0.019

4.755 ± 0.013

5.494 ± 0.013

8.276 ± 0.017

5.338 ± 0.01

6.127 ± 0.011

1.191 ± 0.005

2.794 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski