Riemerella anatipestifer RA-CH-1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Riemerella; Riemerella anatipestifer

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9QTD2|J9QTD2_RIEAN Cyclic nucleotide-binding domain-containing protein OS=Riemerella anatipestifer RA-CH-1 OX=1228997 GN=B739_1118 PE=4 SV=1
MM1 pKa = 7.9ADD3 pKa = 3.16IQLKK7 pKa = 8.19ITDD10 pKa = 4.0RR11 pKa = 11.84EE12 pKa = 4.66GVQHH16 pKa = 7.31DD17 pKa = 3.84IVAPTDD23 pKa = 3.19MSMNLMEE30 pKa = 4.91VIRR33 pKa = 11.84AYY35 pKa = 10.14EE36 pKa = 3.86LAEE39 pKa = 4.23EE40 pKa = 4.81GTVGVCGGMAMCAFCQVYY58 pKa = 10.03VLNDD62 pKa = 3.09VPLPEE67 pKa = 6.29KK68 pKa = 10.68GDD70 pKa = 3.59EE71 pKa = 4.17EE72 pKa = 4.15EE73 pKa = 5.1AMLSEE78 pKa = 4.45AFDD81 pKa = 3.52VKK83 pKa = 10.73EE84 pKa = 3.88NSRR87 pKa = 11.84LGCQIHH93 pKa = 5.78ITEE96 pKa = 5.18EE97 pKa = 3.87IDD99 pKa = 3.49GLEE102 pKa = 4.09VEE104 pKa = 4.55IAPYY108 pKa = 10.25SS109 pKa = 3.52

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9QTA0|J9QTA0_RIEAN Fructose-bisphosphate aldolase OS=Riemerella anatipestifer RA-CH-1 OX=1228997 GN=B739_0956 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.55RR3 pKa = 11.84TFQPSEE9 pKa = 3.58RR10 pKa = 11.84KK11 pKa = 9.37KK12 pKa = 10.2RR13 pKa = 11.84NKK15 pKa = 9.71HH16 pKa = 4.09GFRR19 pKa = 11.84EE20 pKa = 4.33RR21 pKa = 11.84MSTPNGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.45RR41 pKa = 11.84LTVSVSRR48 pKa = 11.84AKK50 pKa = 10.49RR51 pKa = 3.27

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2187

0

2187

686533

37

2340

313.9

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.183 ± 0.053

0.711 ± 0.016

5.157 ± 0.036

7.067 ± 0.064

5.206 ± 0.049

6.297 ± 0.058

1.686 ± 0.024

7.676 ± 0.049

8.523 ± 0.06

9.384 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.024

6.14 ± 0.054

3.355 ± 0.026

3.615 ± 0.036

3.551 ± 0.031

6.416 ± 0.041

5.358 ± 0.039

6.135 ± 0.043

1.027 ± 0.015

4.243 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski