Rosellinia necatrix megabirnavirus 1 (isolate -/Japan/W779/2001) (RnMBV1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Megabirnaviridae; Megabirnavirus; Rosellinia necatrix megabirnavirus 1

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0FZL3|D0FZL3_RMBV1 Uncharacterized protein OS=Rosellinia necatrix megabirnavirus 1 (isolate -/Japan/W779/2001) OX=658904 PE=4 SV=1
MM1 pKa = 7.72LARR4 pKa = 11.84ALCSDD9 pKa = 4.31RR10 pKa = 11.84ALACLKK16 pKa = 10.34SLRR19 pKa = 11.84AGNVARR25 pKa = 11.84IRR27 pKa = 11.84RR28 pKa = 11.84EE29 pKa = 3.24IAVVVKK35 pKa = 9.5VVKK38 pKa = 10.26CCRR41 pKa = 11.84LLVEE45 pKa = 4.43RR46 pKa = 11.84VARR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 9.98VSEE54 pKa = 4.1GVPSGSTDD62 pKa = 3.47VVPLEE67 pKa = 4.12VSGLTEE73 pKa = 3.71ATVFDD78 pKa = 4.52HH79 pKa = 7.46LLLQLVQEE87 pKa = 4.59TLLPSPVSGGAVGVTHH103 pKa = 6.47RR104 pKa = 11.84TAEE107 pKa = 3.99EE108 pKa = 3.8AAPASALQVGTSAGTDD124 pKa = 3.32VIPDD128 pKa = 3.51VVLTSVVSYY137 pKa = 10.36HH138 pKa = 6.56RR139 pKa = 11.84EE140 pKa = 3.8AVEE143 pKa = 4.44HH144 pKa = 6.34IFVAAEE150 pKa = 3.59ARR152 pKa = 11.84AGTRR156 pKa = 11.84EE157 pKa = 4.01VQLLLASVDD166 pKa = 3.63EE167 pKa = 4.46SLVRR171 pKa = 11.84AVYY174 pKa = 8.91PAGLTDD180 pKa = 4.3PVLTLLAHH188 pKa = 7.38DD189 pKa = 4.88YY190 pKa = 11.12ADD192 pKa = 3.96SDD194 pKa = 3.26IGQPFTLHH202 pKa = 6.21YY203 pKa = 10.81SEE205 pKa = 6.16LDD207 pKa = 3.63LGCLSSLTPEE217 pKa = 4.59EE218 pKa = 4.21EE219 pKa = 4.19TGDD222 pKa = 4.21ADD224 pKa = 5.12DD225 pKa = 5.94GYY227 pKa = 12.01

Molecular weight: 24.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0FZL2|D0FZL2_RMBV1 Uncharacterized protein OS=Rosellinia necatrix megabirnavirus 1 (isolate -/Japan/W779/2001) OX=658904 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 4.87GRR4 pKa = 11.84HH5 pKa = 6.37DD6 pKa = 3.75PDD8 pKa = 3.71AALFAQDD15 pKa = 3.36NVYY18 pKa = 10.1FSSVADD24 pKa = 3.5VEE26 pKa = 4.28RR27 pKa = 11.84ATWSDD32 pKa = 2.8IVAKK36 pKa = 10.58ARR38 pKa = 11.84ADD40 pKa = 3.16GWQGRR45 pKa = 11.84DD46 pKa = 3.24VLQEE50 pKa = 3.89FGGGQTLARR59 pKa = 11.84LVRR62 pKa = 11.84NSAIPDD68 pKa = 3.52DD69 pKa = 4.05FRR71 pKa = 11.84NSIVTAKK78 pKa = 9.88GAEE81 pKa = 3.91WLLLRR86 pKa = 11.84SVAHH90 pKa = 5.03VALRR94 pKa = 11.84LSHH97 pKa = 6.75NSYY100 pKa = 11.02KK101 pKa = 10.45KK102 pKa = 10.7LKK104 pKa = 9.87TSILTKK110 pKa = 10.22RR111 pKa = 11.84GCNGSRR117 pKa = 11.84RR118 pKa = 11.84LQAVRR123 pKa = 11.84SVMEE127 pKa = 3.8AAFAGNVPDD136 pKa = 4.12WLAEE140 pKa = 3.84MEE142 pKa = 4.12MLEE145 pKa = 4.29RR146 pKa = 11.84CGPEE150 pKa = 3.62YY151 pKa = 10.86AAVTRR156 pKa = 11.84IVGDD160 pKa = 4.26DD161 pKa = 3.57EE162 pKa = 4.96FDD164 pKa = 3.85PLGTISAVAACEE176 pKa = 4.03GGLNLFVFDD185 pKa = 5.02GPAEE189 pKa = 4.44GGSGHH194 pKa = 5.96EE195 pKa = 4.05AVVVEE200 pKa = 4.51GDD202 pKa = 3.37PGEE205 pKa = 4.3AGAYY209 pKa = 8.03VEE211 pKa = 5.52RR212 pKa = 11.84RR213 pKa = 11.84LKK215 pKa = 10.8LEE217 pKa = 3.84KK218 pKa = 10.16RR219 pKa = 11.84SLAKK223 pKa = 10.47GVINVEE229 pKa = 4.03LKK231 pKa = 10.73KK232 pKa = 11.05VITSSFLLSCEE243 pKa = 4.39VRR245 pKa = 11.84DD246 pKa = 3.72LDD248 pKa = 4.45YY249 pKa = 11.44AKK251 pKa = 10.41HH252 pKa = 6.24CLRR255 pKa = 11.84ILFPITDD262 pKa = 3.47MNLYY266 pKa = 10.3LRR268 pKa = 11.84ARR270 pKa = 11.84LNYY273 pKa = 8.55GCLIRR278 pKa = 11.84VIFKK282 pKa = 10.96LLDD285 pKa = 3.58DD286 pKa = 5.83RR287 pKa = 11.84ILDD290 pKa = 3.99LSHH293 pKa = 7.11LSSVCTNAAGLGHH306 pKa = 6.12QAFACLILTLASGAEE321 pKa = 3.76RR322 pKa = 11.84RR323 pKa = 11.84NCAVVMLQQGIYY335 pKa = 9.0RR336 pKa = 11.84GGMKK340 pKa = 10.11SVEE343 pKa = 3.99KK344 pKa = 10.18RR345 pKa = 11.84LKK347 pKa = 9.98EE348 pKa = 3.43IHH350 pKa = 5.27TAARR354 pKa = 11.84RR355 pKa = 11.84ARR357 pKa = 11.84WLPSYY362 pKa = 10.5LAGVCTDD369 pKa = 4.25PSVMLYY375 pKa = 10.3WNIALGRR382 pKa = 11.84FYY384 pKa = 11.2LDD386 pKa = 3.15YY387 pKa = 10.88AANDD391 pKa = 3.65DD392 pKa = 4.14TVKK395 pKa = 10.93DD396 pKa = 3.25RR397 pKa = 11.84TMYY400 pKa = 9.76MGEE403 pKa = 3.92HH404 pKa = 5.59VAYY407 pKa = 10.14DD408 pKa = 3.51AASRR412 pKa = 11.84MYY414 pKa = 10.69TSDD417 pKa = 2.71QHH419 pKa = 5.89AANMQRR425 pKa = 11.84FFNARR430 pKa = 11.84KK431 pKa = 9.41QDD433 pKa = 3.56AQKK436 pKa = 9.74VWSSSEE442 pKa = 3.84VDD444 pKa = 4.13TLDD447 pKa = 3.16SWLLRR452 pKa = 11.84YY453 pKa = 9.38VQFGSSGSAGGHH465 pKa = 5.98SGDD468 pKa = 4.91EE469 pKa = 3.92FDD471 pKa = 5.08IDD473 pKa = 3.69HH474 pKa = 6.74AVSKK478 pKa = 10.53RR479 pKa = 11.84LWLSNRR485 pKa = 11.84DD486 pKa = 3.41TEE488 pKa = 4.48TIAWYY493 pKa = 9.92VLKK496 pKa = 10.67QPAGASTVTIVKK508 pKa = 10.24RR509 pKa = 11.84EE510 pKa = 4.02AGKK513 pKa = 10.17LRR515 pKa = 11.84QLLPGRR521 pKa = 11.84IPHH524 pKa = 6.95WIVEE528 pKa = 4.24SLLINEE534 pKa = 4.68IEE536 pKa = 4.25SAIYY540 pKa = 10.17RR541 pKa = 11.84SMPMEE546 pKa = 4.98LEE548 pKa = 4.31LDD550 pKa = 3.65AQSEE554 pKa = 4.76YY555 pKa = 10.21TSVMEE560 pKa = 4.0RR561 pKa = 11.84RR562 pKa = 11.84RR563 pKa = 11.84RR564 pKa = 11.84MMLGEE569 pKa = 4.24SVACVDD575 pKa = 3.2WADD578 pKa = 3.9FNITHH583 pKa = 6.27TLKK586 pKa = 11.12DD587 pKa = 3.26MSEE590 pKa = 4.2YY591 pKa = 10.64FLALGEE597 pKa = 4.13AAQRR601 pKa = 11.84ACVEE605 pKa = 4.39NNFVTYY611 pKa = 10.73GGVNYY616 pKa = 10.58GKK618 pKa = 11.15GEE620 pKa = 3.9FLSLCANWCSKK631 pKa = 10.93ALYY634 pKa = 10.62NMWLKK639 pKa = 11.13NGADD643 pKa = 3.51KK644 pKa = 11.3DD645 pKa = 3.89SDD647 pKa = 4.2YY648 pKa = 10.95EE649 pKa = 3.95AHH651 pKa = 7.41ARR653 pKa = 11.84GLWSGWRR660 pKa = 11.84TTSFINCSFNVAYY673 pKa = 10.49CEE675 pKa = 4.61SNFDD679 pKa = 3.91SMHH682 pKa = 6.86HH683 pKa = 6.39IFNSDD688 pKa = 2.96MPLYY692 pKa = 9.86RR693 pKa = 11.84VHH695 pKa = 8.05AGDD698 pKa = 5.47DD699 pKa = 3.67FFGTYY704 pKa = 10.25AEE706 pKa = 5.31EE707 pKa = 3.98IDD709 pKa = 3.3AMRR712 pKa = 11.84FVSSMPLAKK721 pKa = 10.35HH722 pKa = 5.41EE723 pKa = 4.43VNAQKK728 pKa = 10.63QLVGGEE734 pKa = 3.76MGEE737 pKa = 4.3FLRR740 pKa = 11.84HH741 pKa = 5.39EE742 pKa = 4.43YY743 pKa = 10.6FRR745 pKa = 11.84DD746 pKa = 3.47GTVRR750 pKa = 11.84GSVMRR755 pKa = 11.84SIGSFVGSDD764 pKa = 3.29LQAPQLYY771 pKa = 10.37SGVAQCQGANEE782 pKa = 4.96AINLLIRR789 pKa = 11.84RR790 pKa = 11.84GFDD793 pKa = 2.95LRR795 pKa = 11.84AAEE798 pKa = 4.43SIRR801 pKa = 11.84YY802 pKa = 7.67TIVQYY807 pKa = 10.19WGTVKK812 pKa = 10.63EE813 pKa = 4.51PGGSSATPSVKK824 pKa = 10.3LLALSAHH831 pKa = 6.35SGGLGCPRR839 pKa = 11.84FGEE842 pKa = 4.26LATVTRR848 pKa = 11.84EE849 pKa = 3.88RR850 pKa = 11.84LHH852 pKa = 6.83GIPVMRR858 pKa = 11.84DD859 pKa = 3.33VLSKK863 pKa = 10.77LAAPRR868 pKa = 11.84NMEE871 pKa = 4.17RR872 pKa = 11.84LLHH875 pKa = 6.62AFQAKK880 pKa = 8.79IRR882 pKa = 11.84RR883 pKa = 11.84FGYY886 pKa = 9.79IEE888 pKa = 4.06EE889 pKa = 4.87GFTALRR895 pKa = 11.84NAAYY899 pKa = 9.97NATYY903 pKa = 10.52GSDD906 pKa = 3.52LPRR909 pKa = 11.84EE910 pKa = 4.29IKK912 pKa = 10.84LEE914 pKa = 4.05ADD916 pKa = 3.43GEE918 pKa = 4.22LRR920 pKa = 11.84RR921 pKa = 11.84AQVAWINEE929 pKa = 4.29CNLKK933 pKa = 10.0VPEE936 pKa = 4.51MTVAWYY942 pKa = 8.88PSVPSKK948 pKa = 10.69VLEE951 pKa = 3.94YY952 pKa = 10.9LEE954 pKa = 5.17RR955 pKa = 11.84LIACAEE961 pKa = 4.08KK962 pKa = 10.68GLEE965 pKa = 4.19PEE967 pKa = 4.51YY968 pKa = 10.62PMHH971 pKa = 7.34DD972 pKa = 4.01LNGMAVAARR981 pKa = 11.84ATALGAFSALDD992 pKa = 3.23SGLNFLGDD1000 pKa = 3.34EE1001 pKa = 4.52AGRR1004 pKa = 11.84VAGFDD1009 pKa = 3.24AVVAMSRR1016 pKa = 11.84GRR1018 pKa = 11.84AGTILNRR1025 pKa = 11.84MRR1027 pKa = 11.84LYY1029 pKa = 10.75LPEE1032 pKa = 4.8IAVRR1036 pKa = 11.84ALVEE1040 pKa = 4.2HH1041 pKa = 7.04RR1042 pKa = 11.84WNVPTSTGGVVPAQLRR1058 pKa = 11.84GIVFSGLNYY1067 pKa = 10.49ALWYY1071 pKa = 9.23AKK1073 pKa = 10.23NWMPVNAGSLDD1084 pKa = 3.55WLTSYY1089 pKa = 11.14CDD1091 pKa = 3.3AVNIRR1096 pKa = 11.84IANLYY1101 pKa = 10.01KK1102 pKa = 10.64SSLSKK1107 pKa = 9.75MMKK1110 pKa = 8.62MM1111 pKa = 4.54

Molecular weight: 123.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4004

227

1426

1001.0

108.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.464 ± 0.555

1.374 ± 0.259

5.794 ± 0.323

4.82 ± 0.51

3.571 ± 0.466

8.966 ± 0.458

1.998 ± 0.306

3.796 ± 0.218

2.473 ± 0.529

9.565 ± 0.777

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.772 ± 0.383

4.071 ± 0.669

4.121 ± 0.408

2.922 ± 0.185

7.243 ± 0.165

6.069 ± 0.433

6.094 ± 0.752

8.342 ± 0.786

1.499 ± 0.194

3.047 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski