Klebsiella phage KPN3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; unclassified Przondovirus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NAT3|A0A5B9NAT3_9CAUD Uncharacterized protein OS=Klebsiella phage KPN3 OX=2601621 GN=KPN3_25 PE=4 SV=1
MM1 pKa = 8.1LIPFITTASGVAIEE15 pKa = 4.67NAFATIGNVSCNTYY29 pKa = 8.65TLAVGTEE36 pKa = 3.92EE37 pKa = 4.33AQVKK41 pKa = 10.05RR42 pKa = 11.84SSEE45 pKa = 3.91YY46 pKa = 10.21TIAIYY51 pKa = 10.41LSEE54 pKa = 4.05EE55 pKa = 4.39MYY57 pKa = 10.9SAGAKK62 pKa = 9.29PVEE65 pKa = 4.31TRR67 pKa = 11.84SIVLDD72 pKa = 4.35GYY74 pKa = 10.24PRR76 pKa = 11.84DD77 pKa = 4.09LKK79 pKa = 11.22PEE81 pKa = 4.34DD82 pKa = 3.49VLSAQEE88 pKa = 3.85VV89 pKa = 3.53

Molecular weight: 9.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NC00|A0A5B9NC00_9CAUD Uncharacterized protein OS=Klebsiella phage KPN3 OX=2601621 GN=KPN3_26 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84LHH4 pKa = 6.7FNKK7 pKa = 10.46SNGIFSVRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.04DD18 pKa = 3.55RR19 pKa = 11.84STVAASEE26 pKa = 4.13RR27 pKa = 11.84HH28 pKa = 4.99GKK30 pKa = 8.95IPRR33 pKa = 11.84IGDD36 pKa = 3.66TFEE39 pKa = 4.6LAPSVHH45 pKa = 6.56ILVTRR50 pKa = 11.84GLYY53 pKa = 9.93EE54 pKa = 4.12LAQTKK59 pKa = 9.86SRR61 pKa = 11.84PFVPVVVTKK70 pKa = 9.98WPRR73 pKa = 11.84LRR75 pKa = 11.84LFWEE79 pKa = 4.85RR80 pKa = 11.84IKK82 pKa = 11.05EE83 pKa = 4.29VVNEE87 pKa = 4.05

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

11087

36

1321

246.4

27.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.704 ± 0.538

0.956 ± 0.176

6.133 ± 0.225

6.72 ± 0.434

3.725 ± 0.227

7.901 ± 0.468

1.741 ± 0.179

4.925 ± 0.182

6.638 ± 0.443

8.217 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.155

4.176 ± 0.292

3.653 ± 0.191

4.492 ± 0.351

5.583 ± 0.305

6.422 ± 0.415

5.601 ± 0.251

6.972 ± 0.367

1.335 ± 0.173

3.265 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski