Leuconostocaceae bacterium R-53105

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; unclassified Lactobacillaceae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1574 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6H475|A0A1G6H475_9LACO ATP-dependent RNA helicase DeaD OS=Leuconostocaceae bacterium R-53105 OX=1855370 GN=SAMN05216341_10341 PE=3 SV=1
MM1 pKa = 7.93EE2 pKa = 6.41DD3 pKa = 3.39NFTTFAKK10 pKa = 10.51SYY12 pKa = 11.46DD13 pKa = 3.73EE14 pKa = 5.98LFDD17 pKa = 4.23NDD19 pKa = 4.94MYY21 pKa = 10.93QAWAQYY27 pKa = 9.97VKK29 pKa = 10.92ALTKK33 pKa = 10.12PSPLLDD39 pKa = 3.91LGGGAGRR46 pKa = 11.84LAVLLEE52 pKa = 3.95QAGYY56 pKa = 10.17QVDD59 pKa = 4.83LLDD62 pKa = 5.04LSPNMLQLAQAHH74 pKa = 5.69AQQAGVDD81 pKa = 3.8LRR83 pKa = 11.84LIEE86 pKa = 4.49GDD88 pKa = 3.39MRR90 pKa = 11.84DD91 pKa = 3.63FSDD94 pKa = 4.1WPTQYY99 pKa = 10.94PIITSFADD107 pKa = 3.29SLNYY111 pKa = 10.32LPNLDD116 pKa = 4.46DD117 pKa = 6.45LEE119 pKa = 5.44DD120 pKa = 3.93AFQQVFDD127 pKa = 4.36HH128 pKa = 6.57LMPSGLFLFDD138 pKa = 4.5VITPYY143 pKa = 10.34QVNVGYY149 pKa = 10.83DD150 pKa = 3.32NYY152 pKa = 11.07CYY154 pKa = 11.1NNDD157 pKa = 4.44DD158 pKa = 3.65NPEE161 pKa = 4.33NIFMWTSFPGEE172 pKa = 4.1NEE174 pKa = 3.65NSVDD178 pKa = 3.43HH179 pKa = 6.86DD180 pKa = 4.46LKK182 pKa = 10.99FFTYY186 pKa = 10.6NEE188 pKa = 3.89EE189 pKa = 3.78LDD191 pKa = 3.73AFNLLRR197 pKa = 11.84EE198 pKa = 4.27VHH200 pKa = 6.6HH201 pKa = 5.93EE202 pKa = 3.83QSYY205 pKa = 9.06EE206 pKa = 3.78QAVYY210 pKa = 10.88CEE212 pKa = 4.55LLQKK216 pKa = 10.86VGFNQIEE223 pKa = 3.89ISADD227 pKa = 3.29FGQTVPNSQTTRR239 pKa = 11.84WFFKK243 pKa = 10.51ASKK246 pKa = 10.51GDD248 pKa = 3.56TKK250 pKa = 11.37

Molecular weight: 28.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6IRS7|A0A1G6IRS7_9LACO Acetyl esterase/lipase OS=Leuconostocaceae bacterium R-53105 OX=1855370 GN=SAMN05216341_11215 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.19KK9 pKa = 7.37RR10 pKa = 11.84HH11 pKa = 5.5RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 5.84GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.23VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1574

0

1574

476006

39

2928

302.4

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.861 ± 0.09

0.265 ± 0.011

5.886 ± 0.06

5.228 ± 0.07

4.131 ± 0.056

6.651 ± 0.061

2.008 ± 0.026

6.993 ± 0.074

5.608 ± 0.065

10.13 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.035

4.802 ± 0.05

3.653 ± 0.038

5.749 ± 0.079

3.903 ± 0.052

6.266 ± 0.133

5.842 ± 0.062

7.119 ± 0.058

1.071 ± 0.026

3.318 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski