Mycobacterium phage Scarlett

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M568|A0A3G3M568_9CAUD Uncharacterized protein OS=Mycobacterium phage Scarlett OX=2419615 GN=50 PE=4 SV=1
MM1 pKa = 7.36SALGEE6 pKa = 4.23VWVLDD11 pKa = 3.92VEE13 pKa = 4.56ATGPEE18 pKa = 4.1GGDD21 pKa = 3.24YY22 pKa = 11.13DD23 pKa = 4.08GWQSVHH29 pKa = 6.61ASRR32 pKa = 11.84EE33 pKa = 4.05GAIGRR38 pKa = 11.84LVDD41 pKa = 3.61KK42 pKa = 10.72LVEE45 pKa = 4.09YY46 pKa = 10.93GLGDD50 pKa = 3.73VEE52 pKa = 4.52PQGSATADD60 pKa = 3.51NGSMAGDD67 pKa = 3.6YY68 pKa = 9.4EE69 pKa = 4.79VNGVEE74 pKa = 4.14VSYY77 pKa = 10.65GVHH80 pKa = 6.35RR81 pKa = 11.84MPVEE85 pKa = 3.76PP86 pKa = 5.14

Molecular weight: 9.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M6X1|A0A3G3M6X1_9CAUD Uncharacterized protein OS=Mycobacterium phage Scarlett OX=2419615 GN=88 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.09RR3 pKa = 11.84TRR5 pKa = 11.84VYY7 pKa = 9.9RR8 pKa = 11.84APAVEE13 pKa = 4.03VEE15 pKa = 4.1RR16 pKa = 11.84PEE18 pKa = 4.34VVVHH22 pKa = 6.31GRR24 pKa = 11.84VLEE27 pKa = 4.52PGTEE31 pKa = 3.84VSIAGEE37 pKa = 3.73RR38 pKa = 11.84GRR40 pKa = 11.84FRR42 pKa = 11.84FVKK45 pKa = 10.05SARR48 pKa = 11.84TTSGRR53 pKa = 11.84VTCDD57 pKa = 4.04FIGPDD62 pKa = 4.12DD63 pKa = 3.76QTKK66 pKa = 9.74CWRR69 pKa = 11.84SFYY72 pKa = 9.91PEE74 pKa = 4.32RR75 pKa = 11.84IKK77 pKa = 9.98TVHH80 pKa = 6.33RR81 pKa = 11.84LNRR84 pKa = 11.84TRR86 pKa = 11.84ANAAA90 pKa = 3.2

Molecular weight: 10.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19070

40

1358

187.0

20.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.618 ± 0.487

1.138 ± 0.142

6.413 ± 0.282

5.674 ± 0.322

2.611 ± 0.19

9.004 ± 0.519

2.26 ± 0.178

3.692 ± 0.148

3.104 ± 0.214

8.479 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.1

2.522 ± 0.136

5.763 ± 0.292

3.052 ± 0.135

6.995 ± 0.306

5.123 ± 0.184

5.852 ± 0.205

8.002 ± 0.246

2.008 ± 0.113

2.533 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski