Rhodovulum steppense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodovulum

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3581 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1YVV8|A0A4R1YVV8_9RHOB Uncharacterized protein DUF4167 OS=Rhodovulum steppense OX=540251 GN=EV216_108127 PE=4 SV=1
MM1 pKa = 7.44IQMTEE6 pKa = 3.71AARR9 pKa = 11.84DD10 pKa = 3.93AIASAIAGAGQPIAGLRR27 pKa = 11.84LMVQAGGCAGLQYY40 pKa = 11.23TMALEE45 pKa = 4.31LTRR48 pKa = 11.84EE49 pKa = 4.16DD50 pKa = 4.07DD51 pKa = 4.09DD52 pKa = 5.95AVVQADD58 pKa = 4.02GVIVLIDD65 pKa = 4.31PGSQGYY71 pKa = 8.93LVGTTIDD78 pKa = 3.61FVTGLEE84 pKa = 4.14GSGFVFDD91 pKa = 5.2NPNASAGCGCGKK103 pKa = 10.13SFCC106 pKa = 4.85

Molecular weight: 10.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R1YPE6|A0A4R1YPE6_9RHOB Cytosine-specific methyltransferase OS=Rhodovulum steppense OX=540251 GN=EV216_12011 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.51ILNARR34 pKa = 11.84RR35 pKa = 11.84TRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.13RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3581

0

3581

1093594

25

2761

305.4

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.864 ± 0.058

0.883 ± 0.015

5.799 ± 0.032

5.929 ± 0.035

3.539 ± 0.024

9.041 ± 0.039

2.018 ± 0.021

4.786 ± 0.028

2.426 ± 0.028

10.46 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.612 ± 0.018

2.105 ± 0.019

5.58 ± 0.038

2.834 ± 0.02

7.971 ± 0.039

4.459 ± 0.024

5.114 ± 0.025

7.242 ± 0.034

1.373 ± 0.017

1.964 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski