Paenibacillus macerans (Bacillus macerans)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090Y5E6|A0A090Y5E6_PAEMA Uncharacterized protein OS=Paenibacillus macerans OX=44252 GN=DJ90_3023 PE=4 SV=1
MM1 pKa = 7.57PHH3 pKa = 6.43NADD6 pKa = 3.81DD7 pKa = 4.21KK8 pKa = 10.48EE9 pKa = 4.08LRR11 pKa = 11.84PASGPYY17 pKa = 9.19PDD19 pKa = 5.9QVSLWIGRR27 pKa = 11.84FASEE31 pKa = 4.12EE32 pKa = 3.91ALDD35 pKa = 3.7EE36 pKa = 4.15YY37 pKa = 11.58LRR39 pKa = 11.84FDD41 pKa = 4.0YY42 pKa = 11.01SDD44 pKa = 3.43EE45 pKa = 4.16AKK47 pKa = 10.53QRR49 pKa = 11.84DD50 pKa = 3.79PQICRR55 pKa = 11.84DD56 pKa = 3.42FRR58 pKa = 11.84MEE60 pKa = 4.94SFDD63 pKa = 3.5EE64 pKa = 4.24DD65 pKa = 3.28FRR67 pKa = 11.84EE68 pKa = 4.26AFLVEE73 pKa = 4.35TDD75 pKa = 4.45QSNCANWLEE84 pKa = 4.37GCSYY88 pKa = 11.28DD89 pKa = 5.19DD90 pKa = 3.52QLISQFEE97 pKa = 3.98ASGWPEE103 pKa = 3.78PPEE106 pKa = 4.24LEE108 pKa = 4.14GMNAVILLYY117 pKa = 10.39DD118 pKa = 3.36YY119 pKa = 10.98SYY121 pKa = 11.55DD122 pKa = 3.32GTVPVVQNSQYY133 pKa = 9.83SVRR136 pKa = 11.84FIGRR140 pKa = 11.84VVYY143 pKa = 10.67EE144 pKa = 4.26DD145 pKa = 3.26

Molecular weight: 16.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090Y3Y6|A0A090Y3Y6_PAEMA Branched-chain amino acid transport system / permease component family protein OS=Paenibacillus macerans OX=44252 GN=DJ90_2861 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 8.23TGKK9 pKa = 9.57KK10 pKa = 8.75RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.3KK14 pKa = 10.38ARR16 pKa = 11.84RR17 pKa = 11.84TARR20 pKa = 11.84KK21 pKa = 8.11PDD23 pKa = 3.52RR24 pKa = 11.84PVKK27 pKa = 9.07STKK30 pKa = 10.0TEE32 pKa = 3.9TRR34 pKa = 11.84SSSRR38 pKa = 11.84KK39 pKa = 9.36PPGSTLNGQPRR50 pKa = 11.84RR51 pKa = 11.84SQIRR55 pKa = 11.84QLRR58 pKa = 11.84QQRR61 pKa = 11.84NRR63 pKa = 11.84LRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 9.27KK68 pKa = 9.76RR69 pKa = 11.84NKK71 pKa = 9.46RR72 pKa = 11.84KK73 pKa = 10.07SRR75 pKa = 11.84TFGSGLALFF84 pKa = 4.96

Molecular weight: 10.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6551

0

6551

2036740

29

2120

310.9

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.916 ± 0.038

0.819 ± 0.01

5.044 ± 0.022

6.84 ± 0.034

4.187 ± 0.021

7.834 ± 0.032

1.963 ± 0.013

6.353 ± 0.03

5.306 ± 0.03

10.231 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.015

3.685 ± 0.023

4.244 ± 0.02

3.643 ± 0.018

5.448 ± 0.03

5.956 ± 0.027

5.094 ± 0.025

6.948 ± 0.024

1.282 ± 0.013

3.533 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski