Lachnospiraceae bacterium YSD2013

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2888 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4V747|A0A1G4V747_9FIRM DNA-binding transcriptional regulator MarR family OS=Lachnospiraceae bacterium YSD2013 OX=1520828 GN=SAMN02910339_00368 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.42KK3 pKa = 10.19KK4 pKa = 10.39LLSILAMVTVVIAVTSCGTASDD26 pKa = 4.48GPRR29 pKa = 11.84TATSGNKK36 pKa = 8.3TVSDD40 pKa = 4.75LIEE43 pKa = 3.83EE44 pKa = 4.94RR45 pKa = 11.84IAEE48 pKa = 4.13EE49 pKa = 3.81NAATTGSVASEE60 pKa = 3.98EE61 pKa = 4.33PAASEE66 pKa = 3.87APEE69 pKa = 4.19AKK71 pKa = 7.61EE72 pKa = 3.9TPEE75 pKa = 4.29ANVEE79 pKa = 4.2TLDD82 pKa = 3.71EE83 pKa = 4.37STEE86 pKa = 4.12VTDD89 pKa = 3.83VDD91 pKa = 4.94LDD93 pKa = 3.87LTVLSKK99 pKa = 10.14TMVYY103 pKa = 10.39SEE105 pKa = 5.09VYY107 pKa = 10.38NIMVSPEE114 pKa = 3.97DD115 pKa = 3.89YY116 pKa = 9.72IGKK119 pKa = 9.06SIRR122 pKa = 11.84MNGIYY127 pKa = 9.01NTFIDD132 pKa = 4.48DD133 pKa = 3.8STGIRR138 pKa = 11.84YY139 pKa = 7.63FYY141 pKa = 10.45CIIQDD146 pKa = 3.53ATACCAQGIEE156 pKa = 4.93FEE158 pKa = 4.52LTDD161 pKa = 4.97DD162 pKa = 3.84YY163 pKa = 11.69SFPADD168 pKa = 3.66YY169 pKa = 10.21PEE171 pKa = 5.81SGDD174 pKa = 3.62TVTVVGNFDD183 pKa = 3.32TYY185 pKa = 10.78MEE187 pKa = 4.49GEE189 pKa = 4.23YY190 pKa = 9.71MYY192 pKa = 9.84CTLRR196 pKa = 11.84DD197 pKa = 3.57AVLEE201 pKa = 4.09KK202 pKa = 11.25SNN204 pKa = 3.77

Molecular weight: 22.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4VC51|A0A1G4VC51_9FIRM N-acylneuraminate cytidylyltransferase OS=Lachnospiraceae bacterium YSD2013 OX=1520828 GN=SAMN02910339_00667 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.51KK9 pKa = 8.43KK10 pKa = 9.94SHH12 pKa = 6.18AKK14 pKa = 8.83VHH16 pKa = 5.69GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.77VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 4.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2888

0

2888

989764

40

2797

342.7

38.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.664 ± 0.05

1.411 ± 0.019

6.273 ± 0.034

7.555 ± 0.05

4.446 ± 0.034

6.967 ± 0.039

1.555 ± 0.016

7.372 ± 0.045

7.288 ± 0.037

8.588 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.907 ± 0.023

4.532 ± 0.031

3.158 ± 0.026

2.201 ± 0.019

4.021 ± 0.032

5.985 ± 0.031

5.483 ± 0.038

7.268 ± 0.035

0.905 ± 0.016

4.423 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski