Lactobacillus ruminis (strain ATCC 27782 / RF3)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Ligilactobacillus; Ligilactobacillus ruminis

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2SQT9|G2SQT9_LACRR MFS transporter OS=Lactobacillus ruminis (strain ATCC 27782 / RF3) OX=1069534 GN=LRC_02410 PE=4 SV=1
MM1 pKa = 7.36GNDD4 pKa = 3.57NGCMCGADD12 pKa = 3.83DD13 pKa = 5.96QSLFWVDD20 pKa = 3.35IIGYY24 pKa = 9.72ADD26 pKa = 3.47RR27 pKa = 11.84RR28 pKa = 11.84ADD30 pKa = 3.57NDD32 pKa = 3.23ILATRR37 pKa = 11.84LIVSYY42 pKa = 10.23EE43 pKa = 3.61WDD45 pKa = 3.35KK46 pKa = 11.52CSQEE50 pKa = 4.61SVWNLGCIFF59 pKa = 4.27

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2SLU1|G2SLU1_LACRR ABC transporter permease protein OS=Lactobacillus ruminis (strain ATCC 27782 / RF3) OX=1069534 GN=LRC_04810 PE=4 SV=1
MM1 pKa = 7.67IMRR4 pKa = 11.84FFCFCGTRR12 pKa = 11.84SDD14 pKa = 3.86RR15 pKa = 11.84FKK17 pKa = 11.34ALYY20 pKa = 8.3WDD22 pKa = 3.19KK23 pKa = 11.13TGFVLYY29 pKa = 9.52YY30 pKa = 10.56KK31 pKa = 10.23RR32 pKa = 11.84IEE34 pKa = 4.01NGRR37 pKa = 11.84FQWPRR42 pKa = 11.84NQSEE46 pKa = 4.43VKK48 pKa = 10.24KK49 pKa = 10.89INAHH53 pKa = 5.64QFAQTLGRR61 pKa = 11.84IFRR64 pKa = 11.84RR65 pKa = 11.84GKK67 pKa = 9.82EE68 pKa = 3.94NHH70 pKa = 6.35SSLL73 pKa = 4.46

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1851

0

1851

541482

30

1507

292.5

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.314 ± 0.06

0.997 ± 0.022

5.925 ± 0.052

6.947 ± 0.067

4.489 ± 0.046

6.674 ± 0.048

1.886 ± 0.024

6.993 ± 0.055

7.746 ± 0.057

9.42 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.025

4.707 ± 0.044

3.183 ± 0.028

3.453 ± 0.04

4.443 ± 0.05

6.18 ± 0.051

5.217 ± 0.039

7.055 ± 0.046

0.859 ± 0.018

3.657 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski