Amino acid dipepetide frequency for Ornithorhynchus anatinus (Duckbill platypus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.626AlaAla: 7.626 ± 0.044	1.381AlaCys: 1.381 ± 0.011	3.147AlaAsp: 3.147 ± 0.015	4.991AlaGlu: 4.991 ± 0.023	2.54AlaPhe: 2.54 ± 0.013	5.814AlaGly: 5.814 ± 0.031	1.548AlaHis: 1.548 ± 0.009	2.715AlaIle: 2.715 ± 0.013	3.401AlaLys: 3.401 ± 0.016	7.167AlaLeu: 7.167 ± 0.024	1.389AlaMet: 1.389 ± 0.009	2.055AlaAsn: 2.055 ± 0.009	4.802AlaPro: 4.802 ± 0.03	3.099AlaGln: 3.099 ± 0.017	4.295AlaArg: 4.295 ± 0.022	5.734AlaSer: 5.734 ± 0.022	3.508AlaThr: 3.508 ± 0.016	4.892AlaVal: 4.892 ± 0.017	0.818AlaTrp: 0.818 ± 0.007	1.487AlaTyr: 1.487 ± 0.009	0.0AlaXaa: 0.0 ± 0.0
Cys	1.206CysAla: 1.206 ± 0.011	0.572CysCys: 0.572 ± 0.009	0.978CysAsp: 0.978 ± 0.01	1.194CysGlu: 1.194 ± 0.011	0.809CysPhe: 0.809 ± 0.006	1.578CysGly: 1.578 ± 0.014	0.629CysHis: 0.629 ± 0.006	0.901CysIle: 0.901 ± 0.008	1.051CysLys: 1.051 ± 0.009	2.167CysLeu: 2.167 ± 0.014	0.363CysMet: 0.363 ± 0.004	0.722CysAsn: 0.722 ± 0.007	1.478CysPro: 1.478 ± 0.015	1.029CysGln: 1.029 ± 0.012	1.358CysArg: 1.358 ± 0.01	1.867CysSer: 1.867 ± 0.014	1.042CysThr: 1.042 ± 0.01	1.272CysVal: 1.272 ± 0.011	0.269CysTrp: 0.269 ± 0.004	0.549CysTyr: 0.549 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	2.963AspAla: 2.963 ± 0.015	1.056AspCys: 1.056 ± 0.009	2.704AspAsp: 2.704 ± 0.016	3.456AspGlu: 3.456 ± 0.018	2.138AspPhe: 2.138 ± 0.012	3.795AspGly: 3.795 ± 0.022	1.129AspHis: 1.129 ± 0.008	2.424AspIle: 2.424 ± 0.014	2.377AspLys: 2.377 ± 0.013	5.045AspLeu: 5.045 ± 0.018	0.984AspMet: 0.984 ± 0.008	1.601AspAsn: 1.601 ± 0.009	3.287AspPro: 3.287 ± 0.015	1.84AspGln: 1.84 ± 0.01	2.742AspArg: 2.742 ± 0.013	3.968AspSer: 3.968 ± 0.017	2.23AspThr: 2.23 ± 0.012	3.083AspVal: 3.083 ± 0.019	0.651AspTrp: 0.651 ± 0.007	1.458AspTyr: 1.458 ± 0.009	0.0AspXaa: 0.0 ± 0.0
Glu	5.424GluAla: 5.424 ± 0.026	1.196GluCys: 1.196 ± 0.012	4.295GluAsp: 4.295 ± 0.019	8.16GluGlu: 8.16 ± 0.047	2.024GluPhe: 2.024 ± 0.011	4.527GluGly: 4.527 ± 0.019	1.362GluHis: 1.362 ± 0.009	3.053GluIle: 3.053 ± 0.015	5.078GluLys: 5.078 ± 0.026	6.229GluLeu: 6.229 ± 0.026	1.654GluMet: 1.654 ± 0.011	2.924GluAsn: 2.924 ± 0.015	3.313GluPro: 3.313 ± 0.027	2.954GluGln: 2.954 ± 0.015	4.423GluArg: 4.423 ± 0.021	4.329GluSer: 4.329 ± 0.02	3.368GluThr: 3.368 ± 0.015	4.081GluVal: 4.081 ± 0.016	0.684GluTrp: 0.684 ± 0.006	1.494GluTyr: 1.494 ± 0.01	0.0GluXaa: 0.0 ± 0.0
Phe	2.019PheAla: 2.019 ± 0.012	0.909PheCys: 0.909 ± 0.007	1.7PheAsp: 1.7 ± 0.01	1.948PheGlu: 1.948 ± 0.011	1.632PhePhe: 1.632 ± 0.013	2.325PheGly: 2.325 ± 0.015	1.036PheHis: 1.036 ± 0.008	1.71PheIle: 1.71 ± 0.011	1.7PheLys: 1.7 ± 0.01	4.125PheLeu: 4.125 ± 0.021	0.694PheMet: 0.694 ± 0.007	1.315PheAsn: 1.315 ± 0.008	2.143PhePro: 2.143 ± 0.013	1.756PheGln: 1.756 ± 0.01	2.05PheArg: 2.05 ± 0.012	3.36PheSer: 3.36 ± 0.014	1.973PheThr: 1.973 ± 0.011	2.156PheVal: 2.156 ± 0.011	0.493PheTrp: 0.493 ± 0.005	1.163PheTyr: 1.163 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	5.166GlyAla: 5.166 ± 0.025	1.269GlyCys: 1.269 ± 0.011	3.388GlyAsp: 3.388 ± 0.015	4.479GlyGlu: 4.479 ± 0.02	2.503GlyPhe: 2.503 ± 0.014	6.709GlyGly: 6.709 ± 0.044	1.697GlyHis: 1.697 ± 0.011	2.692GlyIle: 2.692 ± 0.014	3.689GlyLys: 3.689 ± 0.017	6.132GlyLeu: 6.132 ± 0.027	1.234GlyMet: 1.234 ± 0.009	2.358GlyAsn: 2.358 ± 0.011	5.421GlyPro: 5.421 ± 0.039	2.862GlyGln: 2.862 ± 0.014	5.015GlyArg: 5.015 ± 0.026	6.049GlySer: 6.049 ± 0.026	3.646GlyThr: 3.646 ± 0.019	3.757GlyVal: 3.757 ± 0.016	0.915GlyTrp: 0.915 ± 0.007	1.775GlyTyr: 1.775 ± 0.013	0.0GlyXaa: 0.0 ± 0.0
His	1.309HisAla: 1.309 ± 0.01	0.689HisCys: 0.689 ± 0.007	0.862HisAsp: 0.862 ± 0.007	1.213HisGlu: 1.213 ± 0.009	1.049HisPhe: 1.049 ± 0.007	1.586HisGly: 1.586 ± 0.011	0.88HisHis: 0.88 ± 0.011	1.154HisIle: 1.154 ± 0.008	1.153HisLys: 1.153 ± 0.009	2.859HisLeu: 2.859 ± 0.012	0.49HisMet: 0.49 ± 0.005	0.81HisAsn: 0.81 ± 0.006	1.792HisPro: 1.792 ± 0.012	1.174HisGln: 1.174 ± 0.011	1.637HisArg: 1.637 ± 0.01	2.061HisSer: 2.061 ± 0.011	1.187HisThr: 1.187 ± 0.009	1.434HisVal: 1.434 ± 0.009	0.331HisTrp: 0.331 ± 0.004	0.749HisTyr: 0.749 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	2.54IleAla: 2.54 ± 0.013	1.014IleCys: 1.014 ± 0.008	1.952IleAsp: 1.952 ± 0.011	2.441IleGlu: 2.441 ± 0.015	1.837IlePhe: 1.837 ± 0.012	2.214IleGly: 2.214 ± 0.011	1.168IleHis: 1.168 ± 0.008	2.21IleIle: 2.21 ± 0.012	2.436IleLys: 2.436 ± 0.014	4.443IleLeu: 4.443 ± 0.018	0.905IleMet: 0.905 ± 0.007	1.709IleAsn: 1.709 ± 0.01	2.546IlePro: 2.546 ± 0.012	2.161IleGln: 2.161 ± 0.012	2.354IleArg: 2.354 ± 0.011	3.51IleSer: 3.51 ± 0.016	2.417IleThr: 2.417 ± 0.013	2.482IleVal: 2.482 ± 0.013	0.507IleTrp: 0.507 ± 0.005	1.291IleTyr: 1.291 ± 0.008	0.0IleXaa: 0.0 ± 0.0
Lys	3.786LysAla: 3.786 ± 0.018	1.03LysCys: 1.03 ± 0.01	2.984LysAsp: 2.984 ± 0.016	4.863LysGlu: 4.863 ± 0.024	1.655LysPhe: 1.655 ± 0.01	3.188LysGly: 3.188 ± 0.021	1.275LysHis: 1.275 ± 0.01	2.616LysIle: 2.616 ± 0.015	4.414LysLys: 4.414 ± 0.025	4.999LysLeu: 4.999 ± 0.023	1.407LysMet: 1.407 ± 0.009	2.225LysAsn: 2.225 ± 0.013	2.881LysPro: 2.881 ± 0.019	2.492LysGln: 2.492 ± 0.014	3.294LysArg: 3.294 ± 0.017	3.689LysSer: 3.689 ± 0.015	2.903LysThr: 2.903 ± 0.013	3.306LysVal: 3.306 ± 0.014	0.607LysTrp: 0.607 ± 0.005	1.449LysTyr: 1.449 ± 0.011	0.0LysXaa: 0.0 ± 0.0
Leu	6.905LeuAla: 6.905 ± 0.028	2.079LeuCys: 2.079 ± 0.013	4.825LeuAsp: 4.825 ± 0.017	7.004LeuGlu: 7.004 ± 0.027	3.453LeuPhe: 3.453 ± 0.018	6.005LeuGly: 6.005 ± 0.02	2.651LeuHis: 2.651 ± 0.013	3.76LeuIle: 3.76 ± 0.016	5.688LeuLys: 5.688 ± 0.023	10.884LeuLeu: 10.884 ± 0.048	1.88LeuMet: 1.88 ± 0.01	3.466LeuAsn: 3.466 ± 0.014	6.331LeuPro: 6.331 ± 0.024	5.464LeuGln: 5.464 ± 0.023	6.255LeuArg: 6.255 ± 0.026	7.954LeuSer: 7.954 ± 0.026	5.008LeuThr: 5.008 ± 0.018	5.5LeuVal: 5.5 ± 0.02	1.116LeuTrp: 1.116 ± 0.007	2.48LeuTyr: 2.48 ± 0.013	0.0LeuXaa: 0.0 ± 0.0
Met	1.74MetAla: 1.74 ± 0.01	0.361MetCys: 0.361 ± 0.004	1.168MetAsp: 1.168 ± 0.008	1.764MetGlu: 1.764 ± 0.01	0.687MetPhe: 0.687 ± 0.006	1.236MetGly: 1.236 ± 0.008	0.403MetHis: 0.403 ± 0.004	0.812MetIle: 0.812 ± 0.007	1.327MetLys: 1.327 ± 0.009	1.772MetLeu: 1.772 ± 0.012	0.536MetMet: 0.536 ± 0.005	0.84MetAsn: 0.84 ± 0.007	0.942MetPro: 0.942 ± 0.01	0.836MetGln: 0.836 ± 0.007	1.001MetArg: 1.001 ± 0.007	1.426MetSer: 1.426 ± 0.008	1.027MetThr: 1.027 ± 0.008	1.315MetVal: 1.315 ± 0.009	0.221MetTrp: 0.221 ± 0.004	0.536MetTyr: 0.536 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.071AsnAla: 2.071 ± 0.012	0.815AsnCys: 0.815 ± 0.008	1.465AsnAsp: 1.465 ± 0.009	2.146AsnGlu: 2.146 ± 0.011	1.403AsnPhe: 1.403 ± 0.008	2.523AsnGly: 2.523 ± 0.014	0.893AsnHis: 0.893 ± 0.007	1.92AsnIle: 1.92 ± 0.012	1.996AsnLys: 1.996 ± 0.011	3.592AsnLeu: 3.592 ± 0.016	0.812AsnMet: 0.812 ± 0.006	1.383AsnAsn: 1.383 ± 0.01	2.157AsnPro: 2.157 ± 0.011	1.544AsnGln: 1.544 ± 0.009	1.917AsnArg: 1.917 ± 0.01	2.895AsnSer: 2.895 ± 0.013	1.78AsnThr: 1.78 ± 0.011	2.16AsnVal: 2.16 ± 0.01	0.45AsnTrp: 0.45 ± 0.005	1.042AsnTyr: 1.042 ± 0.008	0.0AsnXaa: 0.0 ± 0.0
Pro	5.997ProAla: 5.997 ± 0.032	1.16ProCys: 1.16 ± 0.012	3.111ProAsp: 3.111 ± 0.016	4.526ProGlu: 4.526 ± 0.02	2.0ProPhe: 2.0 ± 0.01	6.409ProGly: 6.409 ± 0.045	1.479ProHis: 1.479 ± 0.01	1.855ProIle: 1.855 ± 0.011	2.725ProLys: 2.725 ± 0.019	5.577ProLeu: 5.577 ± 0.02	0.9ProMet: 0.9 ± 0.007	1.844ProAsn: 1.844 ± 0.011	7.828ProPro: 7.828 ± 0.058	2.74ProGln: 2.74 ± 0.017	4.15ProArg: 4.15 ± 0.023	6.003ProSer: 6.003 ± 0.026	3.146ProThr: 3.146 ± 0.023	3.879ProVal: 3.879 ± 0.017	0.703ProTrp: 0.703 ± 0.006	1.335ProTyr: 1.335 ± 0.009	0.0ProXaa: 0.0 ± 0.0
Gln	3.379GlnAla: 3.379 ± 0.018	0.88GlnCys: 0.88 ± 0.01	2.188GlnAsp: 2.188 ± 0.01	3.598GlnGlu: 3.598 ± 0.019	1.365GlnPhe: 1.365 ± 0.009	2.756GlnGly: 2.756 ± 0.015	1.167GlnHis: 1.167 ± 0.008	1.935GlnIle: 1.935 ± 0.011	2.739GlnLys: 2.739 ± 0.015	4.59GlnLeu: 4.59 ± 0.021	1.044GlnMet: 1.044 ± 0.008	1.739GlnAsn: 1.739 ± 0.01	2.686GlnPro: 2.686 ± 0.018	2.953GlnGln: 2.953 ± 0.036	2.926GlnArg: 2.926 ± 0.016	2.933GlnSer: 2.933 ± 0.014	2.203GlnThr: 2.203 ± 0.011	2.711GlnVal: 2.711 ± 0.013	0.548GlnTrp: 0.548 ± 0.005	1.085GlnTyr: 1.085 ± 0.007	0.0GlnXaa: 0.0 ± 0.0
Arg	4.507ArgAla: 4.507 ± 0.02	1.314ArgCys: 1.314 ± 0.011	3.068ArgAsp: 3.068 ± 0.015	4.381ArgGlu: 4.381 ± 0.022	1.999ArgPhe: 1.999 ± 0.01	4.571ArgGly: 4.571 ± 0.026	1.553ArgHis: 1.553 ± 0.011	2.357ArgIle: 2.357 ± 0.012	3.606ArgLys: 3.606 ± 0.018	5.806ArgLeu: 5.806 ± 0.023	1.165ArgMet: 1.165 ± 0.009	2.09ArgAsn: 2.09 ± 0.01	4.181ArgPro: 4.181 ± 0.026	2.691ArgGln: 2.691 ± 0.015	5.22ArgArg: 5.22 ± 0.028	4.562ArgSer: 4.562 ± 0.024	2.999ArgThr: 2.999 ± 0.015	3.367ArgVal: 3.367 ± 0.015	0.796ArgTrp: 0.796 ± 0.007	1.477ArgTyr: 1.477 ± 0.009	0.0ArgXaa: 0.0 ± 0.0
Ser	5.439SerAla: 5.439 ± 0.02	1.775SerCys: 1.775 ± 0.015	3.832SerAsp: 3.832 ± 0.016	4.902SerGlu: 4.902 ± 0.021	2.989SerPhe: 2.989 ± 0.014	5.909SerGly: 5.909 ± 0.026	1.933SerHis: 1.933 ± 0.01	3.077SerIle: 3.077 ± 0.013	3.769SerLys: 3.769 ± 0.019	8.146SerLeu: 8.146 ± 0.03	1.396SerMet: 1.396 ± 0.01	2.512SerAsn: 2.512 ± 0.014	6.487SerPro: 6.487 ± 0.033	3.487SerGln: 3.487 ± 0.015	4.763SerArg: 4.763 ± 0.022	8.961SerSer: 8.961 ± 0.047	4.086SerThr: 4.086 ± 0.02	4.904SerVal: 4.904 ± 0.018	1.052SerTrp: 1.052 ± 0.008	1.919SerTyr: 1.919 ± 0.01	0.0SerXaa: 0.0 ± 0.0
Thr	3.771ThrAla: 3.771 ± 0.015	1.241ThrCys: 1.241 ± 0.014	2.457ThrAsp: 2.457 ± 0.012	3.319ThrGlu: 3.319 ± 0.015	2.058ThrPhe: 2.058 ± 0.01	3.518ThrGly: 3.518 ± 0.018	1.158ThrHis: 1.158 ± 0.008	2.261ThrIle: 2.261 ± 0.014	2.47ThrLys: 2.47 ± 0.012	5.206ThrLeu: 5.206 ± 0.017	1.003ThrMet: 1.003 ± 0.008	1.606ThrAsn: 1.606 ± 0.009	3.594ThrPro: 3.594 ± 0.02	2.021ThrGln: 2.021 ± 0.01	2.498ThrArg: 2.498 ± 0.012	4.285ThrSer: 4.285 ± 0.019	2.767ThrThr: 2.767 ± 0.025	3.887ThrVal: 3.887 ± 0.018	0.67ThrTrp: 0.67 ± 0.008	1.323ThrTyr: 1.323 ± 0.009	0.0ThrXaa: 0.0 ± 0.0
Val	4.356ValAla: 4.356 ± 0.018	1.431ValCys: 1.431 ± 0.01	2.913ValAsp: 2.913 ± 0.015	3.82ValGlu: 3.82 ± 0.015	2.44ValPhe: 2.44 ± 0.011	3.646ValGly: 3.646 ± 0.017	1.467ValHis: 1.467 ± 0.008	2.816ValIle: 2.816 ± 0.013	3.308ValLys: 3.308 ± 0.016	6.181ValLeu: 6.181 ± 0.02	1.228ValMet: 1.228 ± 0.008	2.218ValAsn: 2.218 ± 0.012	3.66ValPro: 3.66 ± 0.017	2.626ValGln: 2.626 ± 0.012	3.422ValArg: 3.422 ± 0.016	4.805ValSer: 4.805 ± 0.016	3.748ValThr: 3.748 ± 0.018	3.972ValVal: 3.972 ± 0.014	0.712ValTrp: 0.712 ± 0.006	1.599ValTyr: 1.599 ± 0.009	0.0ValXaa: 0.0 ± 0.0
Trp	0.833TrpAla: 0.833 ± 0.006	0.234TrpCys: 0.234 ± 0.003	0.652TrpAsp: 0.652 ± 0.007	0.804TrpGlu: 0.804 ± 0.007	0.433TrpPhe: 0.433 ± 0.004	0.808TrpGly: 0.808 ± 0.007	0.293TrpHis: 0.293 ± 0.004	0.572TrpIle: 0.572 ± 0.006	0.787TrpLys: 0.787 ± 0.006	1.197TrpLeu: 1.197 ± 0.009	0.31TrpMet: 0.31 ± 0.004	0.537TrpAsn: 0.537 ± 0.006	0.555TrpPro: 0.555 ± 0.005	0.52TrpGln: 0.52 ± 0.005	0.774TrpArg: 0.774 ± 0.007	0.888TrpSer: 0.888 ± 0.009	0.695TrpThr: 0.695 ± 0.006	0.68TrpVal: 0.68 ± 0.006	0.196TrpTrp: 0.196 ± 0.003	0.323TrpTyr: 0.323 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.373TyrAla: 1.373 ± 0.008	0.63TyrCys: 0.63 ± 0.005	1.241TyrAsp: 1.241 ± 0.009	1.561TyrGlu: 1.561 ± 0.01	1.193TyrPhe: 1.193 ± 0.009	1.637TyrGly: 1.637 ± 0.01	0.699TyrHis: 0.699 ± 0.006	1.294TyrIle: 1.294 ± 0.009	1.349TyrLys: 1.349 ± 0.012	2.589TyrLeu: 2.589 ± 0.012	0.557TyrMet: 0.557 ± 0.005	1.013TyrAsn: 1.013 ± 0.007	1.284TyrPro: 1.284 ± 0.009	1.176TyrGln: 1.176 ± 0.008	1.615TyrArg: 1.615 ± 0.01	2.06TyrSer: 2.06 ± 0.012	1.383TyrThr: 1.383 ± 0.01	1.53TyrVal: 1.53 ± 0.01	0.35TyrTrp: 0.35 ± 0.005	0.898TyrTyr: 0.898 ± 0.008	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.004

Statistics based on 32824 proteins (22389980 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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