Firmicutes bacterium CAG:170

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R6MMU7|R6MMU7_9FIRM Conserved domain protein OS=Firmicutes bacterium CAG:170 OX=1263006 GN=BN515_01290 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84NRR4 pKa = 11.84AGVILTALLLALLCGCAPARR24 pKa = 11.84PAAVYY29 pKa = 9.21QNPIDD34 pKa = 5.39FYY36 pKa = 11.37YY37 pKa = 10.98CDD39 pKa = 4.9LEE41 pKa = 4.78DD42 pKa = 3.61PHH44 pKa = 8.54GGALGALAKK53 pKa = 9.29QTVDD57 pKa = 3.99LVDD60 pKa = 3.71PEE62 pKa = 4.27ITVEE66 pKa = 4.31EE67 pKa = 4.24ILTRR71 pKa = 11.84YY72 pKa = 9.19LAGPDD77 pKa = 4.05DD78 pKa = 4.54PALAAPFPDD87 pKa = 3.84GLTCEE92 pKa = 4.55SVTILDD98 pKa = 3.57GVLCVRR104 pKa = 11.84FGEE107 pKa = 4.88AYY109 pKa = 10.39AEE111 pKa = 3.96LSGIRR116 pKa = 11.84LSLTNACLTKK126 pKa = 10.25TLLQIPFVEE135 pKa = 4.69SVQIEE140 pKa = 4.48TDD142 pKa = 3.01GGMLTTQTATAFTEE156 pKa = 5.22DD157 pKa = 4.03SFLLNDD163 pKa = 4.55ASDD166 pKa = 4.03TPLSEE171 pKa = 3.98AAAAEE176 pKa = 4.16ASAGEE181 pKa = 4.2AEE183 pKa = 4.44TEE185 pKa = 4.13KK186 pKa = 11.32GG187 pKa = 3.39

Molecular weight:
19.63 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|R6LRL4|R6LRL4_9FIRM Membrane-associated phospholipid phosphatase OS=Firmicutes bacterium CAG:170 OX=1263006 GN=BN515_00570 PE=4 SV=1
MM1 pKa = 7.38QRR3 pKa = 11.84RR4 pKa = 11.84NDD6 pKa = 3.46LAGAIDD12 pKa = 3.82IVVVAVDD19 pKa = 4.2LDD21 pKa = 3.69QARR24 pKa = 11.84VGLDD28 pKa = 2.99AVDD31 pKa = 4.01VVVQLAVLVYY41 pKa = 10.44NAILLFGIRR50 pKa = 11.84FGIRR54 pKa = 11.84FRR56 pKa = 11.84IWIWRR61 pKa = 11.84RR62 pKa = 11.84LFDD65 pKa = 4.28HH66 pKa = 6.57LAIRR70 pKa = 11.84AKK72 pKa = 10.59VIEE75 pKa = 4.25ARR77 pKa = 11.84RR78 pKa = 11.84KK79 pKa = 9.07KK80 pKa = 10.16PVGTIHH86 pKa = 7.45PVMQRR91 pKa = 11.84GNDD94 pKa = 3.36LKK96 pKa = 11.11GAVDD100 pKa = 3.44IVIIALDD107 pKa = 4.11LDD109 pKa = 3.56QARR112 pKa = 11.84IGRR115 pKa = 11.84NAIHH119 pKa = 6.61IVVKK123 pKa = 10.72HH124 pKa = 6.02SVDD127 pKa = 3.68LNNSVIVRR135 pKa = 11.84IRR137 pKa = 11.84LKK139 pKa = 10.76LQMSVLIEE147 pKa = 4.14VIEE150 pKa = 4.49CFRR153 pKa = 11.84KK154 pKa = 7.68TCVIRR159 pKa = 11.84IGHH162 pKa = 6.22PVAAVFNCCIIAGKK176 pKa = 10.08IIVISVFCLIQAVTNHH192 pKa = 5.08TVDD195 pKa = 4.35RR196 pKa = 11.84IVGISVQLKK205 pKa = 7.99QSCMLINTLTNAILAEE221 pKa = 4.11IVPISWIVVVVDD233 pKa = 4.49FLHH236 pKa = 6.6IAKK239 pKa = 10.23ALIAIAVLCKK249 pKa = 10.28VIKK252 pKa = 10.71VSIDD256 pKa = 3.39CKK258 pKa = 10.63PLMIYY263 pKa = 10.08IGRR266 pKa = 11.84TKK268 pKa = 10.15IISRR272 pKa = 11.84AIICFQPRR280 pKa = 11.84AVDD283 pKa = 3.38QLAVLKK289 pKa = 10.74LIGQICEE296 pKa = 3.61EE297 pKa = 4.18ALRR300 pKa = 11.84LSIQALEE307 pKa = 3.99ADD309 pKa = 3.69RR310 pKa = 11.84VEE312 pKa = 4.38VIIAFLTVFIRR323 pKa = 11.84QIPPAGLGHH332 pKa = 6.86AVDD335 pKa = 4.5GVIARR340 pKa = 11.84AVQLDD345 pKa = 3.55QARR348 pKa = 11.84DD349 pKa = 3.44LALTGAGHH357 pKa = 6.13FVEE360 pKa = 5.69LIEE363 pKa = 4.4IFVSFVIQTGDD374 pKa = 4.14LVDD377 pKa = 3.57ALQRR381 pKa = 11.84LIVDD385 pKa = 3.86EE386 pKa = 4.57VVGHH390 pKa = 6.27AVDD393 pKa = 4.66RR394 pKa = 11.84FPAGPRR400 pKa = 11.84AVVQNEE406 pKa = 3.85AVGEE410 pKa = 4.03ILIGFAVEE418 pKa = 3.66MAAARR423 pKa = 11.84MRR425 pKa = 11.84GVFRR429 pKa = 11.84LLIGVNAVLLLEE441 pKa = 4.93GGLAGHH447 pKa = 6.24AVEE450 pKa = 5.92RR451 pKa = 11.84IRR453 pKa = 11.84AQIDD457 pKa = 3.56VVADD461 pKa = 3.87RR462 pKa = 11.84AGVLDD467 pKa = 4.1AQALIIIPLCAVLGGQLHH485 pKa = 6.22AAEE488 pKa = 5.13DD489 pKa = 4.06ADD491 pKa = 4.0GVGRR495 pKa = 11.84IGIDD499 pKa = 3.31GLTLLDD505 pKa = 4.89KK506 pKa = 11.12VALDD510 pKa = 3.53GKK512 pKa = 10.6KK513 pKa = 10.18VVFVQHH519 pKa = 6.55RR520 pKa = 11.84RR521 pKa = 11.84GQRR524 pKa = 11.84EE525 pKa = 3.91VIVRR529 pKa = 11.84HH530 pKa = 5.35RR531 pKa = 11.84QTTDD535 pKa = 2.62VHH537 pKa = 6.2SHH539 pKa = 4.88VLVKK543 pKa = 10.85LEE545 pKa = 4.28LYY547 pKa = 10.37RR548 pKa = 11.84HH549 pKa = 6.36FGLFADD555 pKa = 5.03ALRR558 pKa = 11.84QLEE561 pKa = 4.02QQIPCRR567 pKa = 11.84DD568 pKa = 3.79AGNVAAGNHH577 pKa = 4.89LQQRR581 pKa = 11.84LQLLRR586 pKa = 11.84EE587 pKa = 4.37LDD589 pKa = 3.32RR590 pKa = 11.84HH591 pKa = 5.65HH592 pKa = 6.73VQTEE596 pKa = 3.99NVGNVHH602 pKa = 6.79RR603 pKa = 11.84LAGIDD608 pKa = 3.42HH609 pKa = 7.23MIAVDD614 pKa = 3.98LAVAAGDD621 pKa = 3.65VFRR624 pKa = 11.84VRR626 pKa = 11.84DD627 pKa = 3.44LTRR630 pKa = 11.84PCKK633 pKa = 10.34RR634 pKa = 11.84PVAACRR640 pKa = 11.84VVKK643 pKa = 11.1VEE645 pKa = 4.26DD646 pKa = 3.47RR647 pKa = 11.84LIFAVHH653 pKa = 6.55GDD655 pKa = 3.52LDD657 pKa = 3.71RR658 pKa = 11.84RR659 pKa = 11.84ADD661 pKa = 4.2GIVAGNHH668 pKa = 5.45CGHH671 pKa = 6.35RR672 pKa = 11.84RR673 pKa = 11.84VFQSGAFHH681 pKa = 7.25AGKK684 pKa = 10.12CKK686 pKa = 10.43AVTHH690 pKa = 6.08SFNRR694 pKa = 11.84TCRR697 pKa = 11.84AAAQLKK703 pKa = 10.01RR704 pKa = 11.84DD705 pKa = 3.68VLRR708 pKa = 11.84ADD710 pKa = 3.69RR711 pKa = 11.84NGIFIVGSDD720 pKa = 3.16QGKK723 pKa = 9.7VYY725 pKa = 10.74RR726 pKa = 11.84LAIDD730 pKa = 5.34GIDD733 pKa = 3.31ARR735 pKa = 11.84LGEE738 pKa = 4.4FQAVRR743 pKa = 11.84LDD745 pKa = 3.47HH746 pKa = 6.22VEE748 pKa = 4.15RR749 pKa = 11.84LAADD753 pKa = 3.59DD754 pKa = 4.13RR755 pKa = 11.84LAGQEE760 pKa = 4.01LHH762 pKa = 6.37LHH764 pKa = 6.8ASRR767 pKa = 11.84FDD769 pKa = 3.34AGKK772 pKa = 10.42YY773 pKa = 9.7AVLGDD778 pKa = 3.77RR779 pKa = 11.84ADD781 pKa = 3.69GLVRR785 pKa = 11.84RR786 pKa = 11.84PPCDD790 pKa = 2.66IRR792 pKa = 11.84RR793 pKa = 11.84KK794 pKa = 9.02LRR796 pKa = 11.84RR797 pKa = 11.84AAGHH801 pKa = 6.77ADD803 pKa = 2.85AGGRR807 pKa = 11.84KK808 pKa = 6.91WKK810 pKa = 10.24RR811 pKa = 11.84RR812 pKa = 11.84ADD814 pKa = 3.93RR815 pKa = 11.84QVVIGRR821 pKa = 11.84GDD823 pKa = 3.06QRR825 pKa = 11.84MIEE828 pKa = 4.09LRR830 pKa = 11.84RR831 pKa = 11.84DD832 pKa = 3.22RR833 pKa = 11.84RR834 pKa = 11.84SGNDD838 pKa = 3.17HH839 pKa = 6.15QRR841 pKa = 11.84GADD844 pKa = 3.54GTLVAVGGAVYY855 pKa = 10.25DD856 pKa = 4.21RR857 pKa = 11.84KK858 pKa = 10.32RR859 pKa = 11.84VAALLLGNEE868 pKa = 4.49GGRR871 pKa = 11.84AAAVQIDD878 pKa = 4.13GSHH881 pKa = 6.88AARR884 pKa = 11.84VQHH887 pKa = 6.99DD888 pKa = 3.91LRR890 pKa = 11.84QLFCAAACGEE900 pKa = 4.19RR901 pKa = 11.84LLTAVEE907 pKa = 3.97HH908 pKa = 6.28HH909 pKa = 6.44QDD911 pKa = 3.21NLALRR916 pKa = 11.84RR917 pKa = 11.84DD918 pKa = 3.65ADD920 pKa = 3.58AGAGMAAVIVVGRR933 pKa = 11.84AVHH936 pKa = 6.08NRR938 pKa = 11.84LAVLDD943 pKa = 4.37QIDD946 pKa = 3.55AAADD950 pKa = 3.66GLLDD954 pKa = 5.28LILIGVVFTGAADD967 pKa = 3.29HH968 pKa = 6.67GRR970 pKa = 11.84AILQDD975 pKa = 3.14RR976 pKa = 11.84KK977 pKa = 9.27EE978 pKa = 4.18VRR980 pKa = 11.84RR981 pKa = 11.84CAAVVFDD988 pKa = 4.18TLHH991 pKa = 6.4DD992 pKa = 3.72QRR994 pKa = 11.84AVGSAGRR1001 pKa = 11.84HH1002 pKa = 3.57VVEE1005 pKa = 4.23VRR1007 pKa = 11.84IHH1009 pKa = 6.56ADD1011 pKa = 2.88HH1012 pKa = 6.99SIVMLHH1018 pKa = 6.83IIRR1021 pKa = 11.84RR1022 pKa = 11.84IGRR1025 pKa = 11.84IGMPLLGRR1033 pKa = 11.84RR1034 pKa = 11.84HH1035 pKa = 5.82LVGDD1039 pKa = 3.72TGHH1042 pKa = 6.96RR1043 pKa = 11.84PAEE1046 pKa = 3.76RR1047 pKa = 11.84RR1048 pKa = 11.84IICVIVRR1055 pKa = 11.84VDD1057 pKa = 3.1VHH1059 pKa = 7.62IVAGDD1064 pKa = 3.38IDD1066 pKa = 4.49RR1067 pKa = 11.84GNVIDD1072 pKa = 5.49DD1073 pKa = 4.28LLIVLCQRR1081 pKa = 11.84VVDD1084 pKa = 4.46RR1085 pKa = 11.84LRR1087 pKa = 11.84HH1088 pKa = 5.4AGNDD1092 pKa = 2.77RR1093 pKa = 11.84RR1094 pKa = 11.84RR1095 pKa = 11.84IRR1097 pKa = 11.84RR1098 pKa = 11.84EE1099 pKa = 3.47HH1100 pKa = 6.62GIVSIIRR1107 pKa = 11.84VGFRR1111 pKa = 11.84IGLRR1115 pKa = 11.84VRR1117 pKa = 11.84IDD1119 pKa = 2.95RR1120 pKa = 11.84VGAVRR1125 pKa = 11.84VAGQIEE1131 pKa = 4.32QIVRR1135 pKa = 11.84KK1136 pKa = 10.12GIAARR1141 pKa = 11.84LAEE1144 pKa = 4.14RR1145 pKa = 11.84LIVGKK1150 pKa = 10.18VGQRR1154 pKa = 11.84IRR1156 pKa = 11.84AFQRR1160 pKa = 11.84TDD1162 pKa = 2.92QGIPGISAQDD1172 pKa = 3.8LAPDD1176 pKa = 4.09TVLGDD1181 pKa = 3.93HH1182 pKa = 6.95PAEE1185 pKa = 4.15AVGQEE1190 pKa = 3.54IRR1192 pKa = 11.84RR1193 pKa = 11.84AAFIRR1198 pKa = 11.84KK1199 pKa = 6.88TSRR1202 pKa = 11.84KK1203 pKa = 9.4VILSEE1208 pKa = 3.86RR1209 pKa = 11.84AEE1211 pKa = 4.08ASAAIHH1217 pKa = 5.91LRR1219 pKa = 11.84CEE1221 pKa = 3.69IEE1223 pKa = 4.64RR1224 pKa = 11.84IHH1226 pKa = 7.4IAIEE1230 pKa = 3.6VGIAKK1235 pKa = 10.23RR1236 pKa = 11.84MIDD1239 pKa = 3.91LIEE1242 pKa = 4.27RR1243 pKa = 11.84AVGHH1247 pKa = 6.88AEE1249 pKa = 4.11VSCPDD1254 pKa = 3.28VGRR1257 pKa = 11.84KK1258 pKa = 8.36NVHH1261 pKa = 4.42IRR1263 pKa = 11.84LRR1265 pKa = 11.84GVYY1268 pKa = 8.62SVRR1271 pKa = 11.84NVHH1274 pKa = 6.09VLGAVHH1280 pKa = 6.01QVGDD1284 pKa = 3.57IAGPAAHH1291 pKa = 6.63VGAGLYY1297 pKa = 10.69GGFRR1301 pKa = 11.84HH1302 pKa = 6.39ALHH1305 pKa = 6.37LRR1307 pKa = 11.84VVRR1310 pKa = 11.84HH1311 pKa = 4.76IHH1313 pKa = 5.15RR1314 pKa = 11.84TEE1316 pKa = 3.63HH1317 pKa = 5.22MAEE1320 pKa = 3.97VSRR1323 pKa = 11.84IAVGQRR1329 pKa = 11.84KK1330 pKa = 8.72VLDD1333 pKa = 3.25VLQRR1337 pKa = 11.84RR1338 pKa = 11.84RR1339 pKa = 11.84RR1340 pKa = 11.84GSVRR1344 pKa = 11.84CVLLGNIGRR1353 pKa = 11.84KK1354 pKa = 9.7GCVFLAADD1362 pKa = 4.58HH1363 pKa = 6.56LPCAGRR1369 pKa = 11.84VALNARR1375 pKa = 11.84ADD1377 pKa = 3.79EE1378 pKa = 4.38VDD1380 pKa = 3.83DD1381 pKa = 4.24EE1382 pKa = 5.56RR1383 pKa = 11.84IDD1385 pKa = 3.41VLARR1389 pKa = 11.84IFRR1392 pKa = 11.84RR1393 pKa = 11.84VGLGVALQEE1402 pKa = 3.88LQARR1406 pKa = 11.84KK1407 pKa = 8.54EE1408 pKa = 3.92RR1409 pKa = 11.84RR1410 pKa = 11.84VVRR1413 pKa = 11.84NRR1415 pKa = 11.84LRR1417 pKa = 11.84SRR1419 pKa = 11.84RR1420 pKa = 11.84LLLHH1424 pKa = 6.38RR1425 pKa = 11.84EE1426 pKa = 4.04RR1427 pKa = 11.84FIKK1430 pKa = 9.82WLLRR1434 pKa = 11.84KK1435 pKa = 9.88LFLRR1439 pKa = 11.84PGPDD1443 pKa = 2.87ILLAGCKK1450 pKa = 9.63RR1451 pKa = 11.84LFVVLLHH1458 pKa = 6.41IPARR1462 pKa = 11.84LLVRR1466 pKa = 11.84RR1467 pKa = 11.84IRR1469 pKa = 11.84GHH1471 pKa = 4.43GQQRR1475 pKa = 11.84RR1476 pKa = 11.84QQQYY1480 pKa = 7.24QDD1482 pKa = 3.0EE1483 pKa = 4.4YY1484 pKa = 10.99RR1485 pKa = 11.84RR1486 pKa = 11.84YY1487 pKa = 10.18ASFRR1491 pKa = 11.84LSASHH1496 pKa = 6.51VLFSFLLFQMIDD1508 pKa = 3.41QIII1511 pKa = 3.6

Molecular weight:
167.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2216

0

2216

674074

29

2520

304.2

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.999 ± 0.055

1.839 ± 0.027

5.613 ± 0.045

6.934 ± 0.051

3.826 ± 0.034

7.555 ± 0.053

1.928 ± 0.03

5.592 ± 0.047

4.739 ± 0.055

9.986 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.031

3.265 ± 0.032

4.037 ± 0.032

3.467 ± 0.031

5.915 ± 0.059

5.556 ± 0.047

5.468 ± 0.041

6.929 ± 0.051

1.016 ± 0.019

3.56 ± 0.038

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski