Paucilactobacillus wasatchensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Paucilactobacillus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D1A8U1|A0A0D1A8U1_9LACO ATP synthase subunit beta OS=Paucilactobacillus wasatchensis OX=1335616 GN=atpD PE=3 SV=1
MM1 pKa = 7.59NKK3 pKa = 9.95RR4 pKa = 11.84LVNDD8 pKa = 3.12EE9 pKa = 4.47TEE11 pKa = 4.1IKK13 pKa = 9.86EE14 pKa = 4.06HH15 pKa = 5.78YY16 pKa = 10.39KK17 pKa = 9.7MYY19 pKa = 10.46KK20 pKa = 10.0AGGRR24 pKa = 11.84WLFASISLITFGSMLMFGTTTSKK47 pKa = 10.92AATEE51 pKa = 3.97NTEE54 pKa = 4.02ADD56 pKa = 3.97GSSSTAVTSSAAFNANDD73 pKa = 3.5STQVLSTTSNSASTEE88 pKa = 4.02TTDD91 pKa = 5.41DD92 pKa = 3.84NNSTTGDD99 pKa = 3.52AAQSEE104 pKa = 5.08VSSAAASNSTVASSSASSTYY124 pKa = 11.32SNATVATSNAASSMLLKK141 pKa = 10.08TDD143 pKa = 3.25ATVAAAATSSITMSSDD159 pKa = 2.76TVGYY163 pKa = 10.62DD164 pKa = 3.85SSNTGNGGVVNIDD177 pKa = 3.15YY178 pKa = 8.45TFTGGADD185 pKa = 3.45DD186 pKa = 4.49TFTITLPADD195 pKa = 3.36SDD197 pKa = 4.33TYY199 pKa = 11.01TLGSIPAMTTGKK211 pKa = 9.0GTMTSVKK218 pKa = 10.2NADD221 pKa = 3.28GSTTITYY228 pKa = 10.47KK229 pKa = 10.97LSTQGSYY236 pKa = 8.39TANLTLNQINNEE248 pKa = 4.16YY249 pKa = 10.03AHH251 pKa = 6.62SSAMTDD257 pKa = 2.09IGTTIKK263 pKa = 10.11TITSTINGVAQTPVTFTQIIQPSMDD288 pKa = 3.34PTGTTRR294 pKa = 11.84LHH296 pKa = 7.16PDD298 pKa = 3.01TGVVTDD304 pKa = 5.35LLPDD308 pKa = 3.28TDD310 pKa = 3.84YY311 pKa = 11.64VYY313 pKa = 10.89QFSVNEE319 pKa = 3.81ADD321 pKa = 3.88GVYY324 pKa = 10.74NDD326 pKa = 5.07GYY328 pKa = 7.96TTAKK332 pKa = 10.06VNSAANYY339 pKa = 9.73GGTTVTIPTPTGFVLDD355 pKa = 4.1SDD357 pKa = 4.85LTNQLNGYY365 pKa = 6.21TSSSGTTITQPDD377 pKa = 4.26GAGGDD382 pKa = 3.76IIISVAASDD391 pKa = 6.09GEE393 pKa = 4.47QNWNSTGSYY402 pKa = 10.24KK403 pKa = 10.69LAGYY407 pKa = 10.78YY408 pKa = 10.43DD409 pKa = 3.64VTQTDD414 pKa = 3.82EE415 pKa = 4.35EE416 pKa = 4.51QTLTAAGPATVTQVLNAAGDD436 pKa = 3.91TLTATGSGVWTDD448 pKa = 4.32SIAATDD454 pKa = 3.71TAEE457 pKa = 4.04VGAATVTASGNSSTASTQLLIDD479 pKa = 4.39GDD481 pKa = 4.28TTNDD485 pKa = 3.56PDD487 pKa = 4.26LLNSFSFTTSTAAPVDD503 pKa = 3.82DD504 pKa = 4.42AQITITIPDD513 pKa = 3.95GLDD516 pKa = 2.91ATGIKK521 pKa = 10.49VPDD524 pKa = 3.78GGANTSAYY532 pKa = 10.75LPGTTSYY539 pKa = 11.32SYY541 pKa = 10.27TLTLADD547 pKa = 4.07GSVEE551 pKa = 4.14TGTVNSGGTITPTDD565 pKa = 3.32SSAIRR570 pKa = 11.84QVVLVPNEE578 pKa = 4.08LAPGASSGTGWLSTGKK594 pKa = 9.86QVFSVYY600 pKa = 9.31GTVSATYY607 pKa = 9.82DD608 pKa = 3.63YY609 pKa = 11.36GSTIPDD615 pKa = 3.62GATLVSTISLSSEE628 pKa = 3.9DD629 pKa = 3.84ANIAEE634 pKa = 4.6MTNSVTQTVIYY645 pKa = 9.01PQDD648 pKa = 3.23AVASVGMYY656 pKa = 8.47VHH658 pKa = 6.72QISKK662 pKa = 8.16TAGYY666 pKa = 8.68EE667 pKa = 3.92NSGSISLVNTGNAGQTTNSVYY688 pKa = 10.47EE689 pKa = 4.0PIYY692 pKa = 10.8YY693 pKa = 9.96FVLPSATTVASITNLPDD710 pKa = 3.71GGVVSYY716 pKa = 7.14FTADD720 pKa = 3.38DD721 pKa = 3.62GRR723 pKa = 11.84EE724 pKa = 4.06VVEE727 pKa = 3.92VDD729 pKa = 3.39YY730 pKa = 10.47TGSGAYY736 pKa = 9.43ISTQEE741 pKa = 4.14TYY743 pKa = 10.86LIQINLTNNSDD754 pKa = 4.14ALPGTYY760 pKa = 8.56ATNLYY765 pKa = 7.97VTSPVTQLKK774 pKa = 8.19QTTPVTDD781 pKa = 3.58TSYY784 pKa = 11.62TNGDD788 pKa = 3.59ANAVSAGSGSWTISSVSTVYY808 pKa = 9.71DD809 pKa = 3.32TTLAQGNNDD818 pKa = 3.83TVPVTDD824 pKa = 4.98ASAEE828 pKa = 4.22RR829 pKa = 11.84TGDD832 pKa = 3.37TTLNFYY838 pKa = 10.9DD839 pKa = 3.65VLGNTSSATATNVSSVINLPTVGDD863 pKa = 3.89ASGSQYY869 pKa = 10.28TFEE872 pKa = 4.16LTGPIDD878 pKa = 4.19LPTSFTTSTGEE889 pKa = 4.14TEE891 pKa = 4.16LTADD895 pKa = 4.19GTPVTAQVLYY905 pKa = 9.06STSLATLNGDD915 pKa = 3.62AADD918 pKa = 3.56TSGYY922 pKa = 8.03VTADD926 pKa = 3.69QITDD930 pKa = 3.17WSAVRR935 pKa = 11.84SILIEE940 pKa = 3.91YY941 pKa = 9.96SAIPSNTSTGRR952 pKa = 11.84ISITGTSTDD961 pKa = 3.47GFEE964 pKa = 4.28GTTDD968 pKa = 3.08EE969 pKa = 4.31TGYY972 pKa = 11.35LEE974 pKa = 4.5TQFSATGYY982 pKa = 8.68DD983 pKa = 3.25TVTRR987 pKa = 11.84TGTDD991 pKa = 3.16NASLTLKK998 pKa = 9.59GTIAALSVVLNPGSLTYY1015 pKa = 10.51DD1016 pKa = 3.83GQSKK1020 pKa = 10.9ASDD1023 pKa = 3.32ATNATVTVTLADD1035 pKa = 3.53GTTQEE1040 pKa = 4.11VSISNAEE1047 pKa = 3.98GKK1049 pKa = 10.2NDD1051 pKa = 3.27FTVANDD1057 pKa = 5.05GINADD1062 pKa = 3.83SYY1064 pKa = 11.87SYY1066 pKa = 11.19ILTDD1070 pKa = 3.17SGIANVEE1077 pKa = 4.1KK1078 pKa = 10.79AVGSTIAATDD1088 pKa = 3.42VQGTIDD1094 pKa = 3.8ILSLKK1099 pKa = 9.9TSISLNDD1106 pKa = 3.53GGFTYY1111 pKa = 10.93NGDD1114 pKa = 3.85NASSASGLTASVALADD1130 pKa = 4.21GSIKK1134 pKa = 9.75TVALTSADD1142 pKa = 3.1ITVTADD1148 pKa = 3.0SSSVGSYY1155 pKa = 10.23KK1156 pKa = 10.69YY1157 pKa = 10.63SLSANGLAAVSAAVGNDD1174 pKa = 3.21YY1175 pKa = 11.0QLNNTTTTGNITITKK1190 pKa = 9.28AAYY1193 pKa = 9.51EE1194 pKa = 4.08FEE1196 pKa = 4.37LTGNEE1201 pKa = 4.18TEE1203 pKa = 4.96SYY1205 pKa = 9.73TGNQQSPDD1213 pKa = 3.3AVNYY1217 pKa = 10.66SVTLPTGEE1225 pKa = 4.52KK1226 pKa = 10.19FVLSDD1231 pKa = 3.69ADD1233 pKa = 3.49IEE1235 pKa = 4.37LVSGGTDD1242 pKa = 2.8VGSYY1246 pKa = 7.98QVQLTEE1252 pKa = 4.89LGKK1255 pKa = 10.24QVLGAIIGTNYY1266 pKa = 10.41DD1267 pKa = 3.0VSFADD1272 pKa = 3.31NATFTIIKK1280 pKa = 9.45AQAAKK1285 pKa = 10.76VSVNDD1290 pKa = 3.36QTIKK1294 pKa = 10.58EE1295 pKa = 4.31GQATPDD1301 pKa = 3.52FNVSYY1306 pKa = 11.24GDD1308 pKa = 4.13GLTSMQLTNSDD1319 pKa = 4.37FEE1321 pKa = 4.36FLGANGQILTEE1332 pKa = 4.26IPTTTGIYY1340 pKa = 8.18TVKK1343 pKa = 10.68LNAAGIAKK1351 pKa = 9.75IEE1353 pKa = 4.07AANPNYY1359 pKa = 9.95EE1360 pKa = 3.84FSEE1363 pKa = 4.59DD1364 pKa = 3.47DD1365 pKa = 4.66FIAGTYY1371 pKa = 9.31TIVSQDD1377 pKa = 3.14MANPGNPGEE1386 pKa = 4.63SGDD1389 pKa = 4.05GGLGTSEE1396 pKa = 3.99VTGTDD1401 pKa = 3.21SSDD1404 pKa = 3.38GSVTSTNSNNQVAEE1418 pKa = 4.1VTTVNKK1424 pKa = 7.8TTGSTKK1430 pKa = 10.4RR1431 pKa = 11.84LPQTGEE1437 pKa = 3.95SEE1439 pKa = 4.19NPEE1442 pKa = 4.05MAEE1445 pKa = 4.13LGLGLMSLFAMFGLLNRR1462 pKa = 11.84KK1463 pKa = 8.4KK1464 pKa = 10.45RR1465 pKa = 11.84KK1466 pKa = 7.45QQ1467 pKa = 3.19

Molecular weight: 150.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D1A750|A0A0D1A750_9LACO Iron-sulfur cluster assembly scaffold protein OS=Paucilactobacillus wasatchensis OX=1335616 GN=iscU PE=4 SV=1
MM1 pKa = 7.2SQLDD5 pKa = 3.82NTLKK9 pKa = 11.03LLGITDD15 pKa = 3.48TNIQVFGTRR24 pKa = 11.84DD25 pKa = 3.34EE26 pKa = 4.19FHH28 pKa = 6.77GRR30 pKa = 11.84GSGRR34 pKa = 11.84KK35 pKa = 8.8KK36 pKa = 10.61YY37 pKa = 10.8LVIQAEE43 pKa = 4.2LTYY46 pKa = 9.65TLRR49 pKa = 11.84RR50 pKa = 11.84CPSCGYY56 pKa = 8.26NTLHH60 pKa = 7.05PNGHH64 pKa = 6.92KK65 pKa = 9.27LTHH68 pKa = 4.86VHH70 pKa = 6.37IAGPMDD76 pKa = 3.53RR77 pKa = 11.84PVILEE82 pKa = 4.03LNKK85 pKa = 9.92QRR87 pKa = 11.84WRR89 pKa = 11.84CSNCHH94 pKa = 5.19SACTATTPVVSTNHH108 pKa = 6.95AIGHH112 pKa = 6.07GLATHH117 pKa = 5.55VLKK120 pKa = 10.73LASKK124 pKa = 9.83SLPAKK129 pKa = 9.67TIASLTGISTNSVQRR144 pKa = 11.84ILTANIHH151 pKa = 4.5THH153 pKa = 5.71ASRR156 pKa = 11.84RR157 pKa = 11.84LPINLCFDD165 pKa = 4.42EE166 pKa = 4.97FRR168 pKa = 11.84STHH171 pKa = 5.06GSMSFICIDD180 pKa = 3.62ADD182 pKa = 3.57THH184 pKa = 6.78KK185 pKa = 10.63SVKK188 pKa = 10.16VLSDD192 pKa = 3.22RR193 pKa = 11.84LNRR196 pKa = 11.84TIKK199 pKa = 10.46QFFLSRR205 pKa = 11.84TGRR208 pKa = 11.84GSTRR212 pKa = 11.84HH213 pKa = 6.17HH214 pKa = 4.99GHH216 pKa = 6.13EE217 pKa = 4.38RR218 pKa = 11.84LLSGIRR224 pKa = 11.84ARR226 pKa = 11.84TIPP229 pKa = 3.49

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1826

0

1826

536271

37

1467

293.7

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.148 ± 0.062

0.454 ± 0.013

5.612 ± 0.051

5.251 ± 0.063

4.278 ± 0.043

6.702 ± 0.053

2.193 ± 0.024

7.34 ± 0.05

6.1 ± 0.055

9.783 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.028

4.893 ± 0.041

3.515 ± 0.029

5.08 ± 0.059

3.89 ± 0.042

6.074 ± 0.067

6.359 ± 0.051

7.28 ± 0.05

0.982 ± 0.023

3.362 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski