Phaeobacter sp. 22II1-1F12B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Phaeobacter; unclassified Phaeobacter

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4790 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A254QXN8|A0A254QXN8_9RHOB Cytochrome P450 OS=Phaeobacter sp. 22II1-1F12B OX=1317111 GN=ATO1_00875 PE=3 SV=1
MM1 pKa = 7.49NYY3 pKa = 9.72VLEE6 pKa = 4.37TDD8 pKa = 3.41NVGVNIQDD16 pKa = 3.28IALSGDD22 pKa = 3.22FTIEE26 pKa = 3.64FNVYY30 pKa = 9.86FEE32 pKa = 5.12PGSEE36 pKa = 3.67IAKK39 pKa = 10.15NDD41 pKa = 4.38GIVSSNVDD49 pKa = 3.21GGNDD53 pKa = 3.02INFFGGIPRR62 pKa = 11.84LYY64 pKa = 9.89DD65 pKa = 3.79ANWGDD70 pKa = 3.92VVVGTSALNTGEE82 pKa = 4.03WNHH85 pKa = 5.91IAFVRR90 pKa = 11.84EE91 pKa = 3.99GGVVRR96 pKa = 11.84LYY98 pKa = 11.61VNGVNDD104 pKa = 4.68ANSTTANYY112 pKa = 9.83TDD114 pKa = 3.61VFNISTLATSVAGALEE130 pKa = 4.06GRR132 pKa = 11.84LDD134 pKa = 3.76EE135 pKa = 4.26VRR137 pKa = 11.84IWSLARR143 pKa = 11.84PGAEE147 pKa = 3.53VLANADD153 pKa = 3.34GALNLSGGVPVGLEE167 pKa = 3.43RR168 pKa = 11.84YY169 pKa = 9.52YY170 pKa = 11.13RR171 pKa = 11.84FDD173 pKa = 4.68DD174 pKa = 5.09DD175 pKa = 3.82IGHH178 pKa = 6.68IVDD181 pKa = 3.4ATGNAEE187 pKa = 4.24TGHH190 pKa = 6.91DD191 pKa = 3.87LHH193 pKa = 7.08PPTGTSIIQDD203 pKa = 3.36DD204 pKa = 4.76TVFDD208 pKa = 4.12TPVGDD213 pKa = 3.25VGGFIDD219 pKa = 3.89TKK221 pKa = 10.87VVTGLVLPTDD231 pKa = 4.15LAFLPDD237 pKa = 3.34GRR239 pKa = 11.84ALVIQKK245 pKa = 10.21SGEE248 pKa = 3.97VLLIEE253 pKa = 5.17DD254 pKa = 4.36PSVANSTKK262 pKa = 9.69TLYY265 pKa = 9.69MDD267 pKa = 5.5LSAQVEE273 pKa = 4.22NSRR276 pKa = 11.84EE277 pKa = 3.98AGLLNVVVDD286 pKa = 5.01PNFDD290 pKa = 4.07DD291 pKa = 4.07NGYY294 pKa = 10.69FYY296 pKa = 11.57LLYY299 pKa = 10.71TNLDD303 pKa = 3.61EE304 pKa = 5.18QRR306 pKa = 11.84TTVSRR311 pKa = 11.84FQHH314 pKa = 5.36QEE316 pKa = 3.41NAGGSTSTGDD326 pKa = 3.15ISSEE330 pKa = 4.12TVLWRR335 pKa = 11.84EE336 pKa = 3.34FDD338 pKa = 3.54TYY340 pKa = 10.84SEE342 pKa = 4.71KK343 pKa = 10.95DD344 pKa = 3.32HH345 pKa = 6.66QGGGLAVAYY354 pKa = 9.52EE355 pKa = 4.82PIDD358 pKa = 4.13ANDD361 pKa = 3.74PSPYY365 pKa = 10.31KK366 pKa = 10.76LYY368 pKa = 9.27ITVGEE373 pKa = 4.15EE374 pKa = 4.29FEE376 pKa = 4.66GEE378 pKa = 3.76NAADD382 pKa = 3.92LTHH385 pKa = 7.77DD386 pKa = 4.09DD387 pKa = 4.32GKK389 pKa = 10.21VHH391 pKa = 7.77RR392 pKa = 11.84INLTDD397 pKa = 3.96GSIPTDD403 pKa = 3.26NPYY406 pKa = 10.86YY407 pKa = 10.56DD408 pKa = 3.82PAVAATYY415 pKa = 8.06TPWINTQTAISTSAANTAIDD435 pKa = 3.93PEE437 pKa = 4.81GVLTTVYY444 pKa = 10.58SYY446 pKa = 11.56GLRR449 pKa = 11.84NPFRR453 pKa = 11.84AEE455 pKa = 3.65YY456 pKa = 9.84DD457 pKa = 3.72QVSGTLWIGEE467 pKa = 4.03VGGNGRR473 pKa = 11.84YY474 pKa = 9.62ASEE477 pKa = 4.76DD478 pKa = 3.14VHH480 pKa = 7.98IAYY483 pKa = 9.26PGANMGWPSEE493 pKa = 4.12EE494 pKa = 5.24GYY496 pKa = 11.08LSDD499 pKa = 5.53PNDD502 pKa = 4.24PGNPIHH508 pKa = 7.14SYY510 pKa = 9.54IHH512 pKa = 6.8ASAPGQDD519 pKa = 3.43QLPSYY524 pKa = 9.73GDD526 pKa = 3.51SGSSISGGVVYY537 pKa = 10.66RR538 pKa = 11.84GTDD541 pKa = 3.62FPEE544 pKa = 4.42EE545 pKa = 3.68FQGAYY550 pKa = 10.14FYY552 pKa = 11.49GDD554 pKa = 3.16WVRR557 pKa = 11.84NWIRR561 pKa = 11.84YY562 pKa = 9.4LEE564 pKa = 4.05VDD566 pKa = 3.77YY567 pKa = 11.6SSGEE571 pKa = 3.99PVVVSDD577 pKa = 4.04NHH579 pKa = 6.25FKK581 pKa = 10.96NATGQVLTFAEE592 pKa = 4.71APDD595 pKa = 3.5GTLYY599 pKa = 11.18YY600 pKa = 9.49ITTFQTGNIFNFEE613 pKa = 3.95GAVNRR618 pKa = 11.84LEE620 pKa = 4.01YY621 pKa = 10.8SSTNTAPAGTGIILDD636 pKa = 4.16PGEE639 pKa = 4.41EE640 pKa = 4.05ASLTAPHH647 pKa = 6.45TVTFEE652 pKa = 3.91TDD654 pKa = 2.92AFDD657 pKa = 5.13PDD659 pKa = 3.82GDD661 pKa = 4.68AITYY665 pKa = 7.36EE666 pKa = 4.18WSFGDD671 pKa = 4.97GPDD674 pKa = 3.74IDD676 pKa = 4.88GDD678 pKa = 4.48GIGDD682 pKa = 4.03GATSTEE688 pKa = 4.08MNPTYY693 pKa = 10.21TYY695 pKa = 10.21TSEE698 pKa = 3.99GQYY701 pKa = 9.53TVEE704 pKa = 5.79LIVTDD709 pKa = 4.01ANGAQTVYY717 pKa = 9.7NPKK720 pKa = 10.37VITVGEE726 pKa = 4.19APVVTINTPTQGQTFRR742 pKa = 11.84AGEE745 pKa = 4.17TVIFTGSAFDD755 pKa = 4.47ANDD758 pKa = 3.66GVLTGEE764 pKa = 4.14DD765 pKa = 3.85VFWSVNLAHH774 pKa = 6.7NEE776 pKa = 4.05HH777 pKa = 6.31YY778 pKa = 10.4HH779 pKa = 6.21PEE781 pKa = 3.92VSAVEE786 pKa = 4.02NTPGGFSFVVPTDD799 pKa = 2.97GHH801 pKa = 6.92DD802 pKa = 3.32YY803 pKa = 10.49YY804 pKa = 11.26EE805 pKa = 4.29NVGYY809 pKa = 9.91QVSLTAIDD817 pKa = 3.75SSGLRR822 pKa = 11.84TTQSVTIFPEE832 pKa = 4.33EE833 pKa = 4.41SITTYY838 pKa = 11.48DD839 pKa = 3.57MPDD842 pKa = 2.76VSGFNFFIDD851 pKa = 4.97GIGYY855 pKa = 10.11SGDD858 pKa = 3.36QVRR861 pKa = 11.84DD862 pKa = 3.58NIIGFEE868 pKa = 4.18HH869 pKa = 6.05TVSVQASYY877 pKa = 11.21IDD879 pKa = 5.35AGFLWEE885 pKa = 4.43FSHH888 pKa = 7.29WEE890 pKa = 4.22DD891 pKa = 5.15DD892 pKa = 3.84PSLTSTSRR900 pKa = 11.84QFIVPEE906 pKa = 4.0TDD908 pKa = 3.04SVLRR912 pKa = 11.84PVYY915 pKa = 10.52AQTDD919 pKa = 3.67VVGPVNAEE927 pKa = 3.7DD928 pKa = 4.63DD929 pKa = 3.92VFARR933 pKa = 11.84DD934 pKa = 3.55LASLNGNQIQTIGNVLLNDD953 pKa = 4.73DD954 pKa = 5.05NPTGDD959 pKa = 3.57PFSVVGLNGYY969 pKa = 9.46KK970 pKa = 8.97VTGAPKK976 pKa = 10.01TDD978 pKa = 3.51DD979 pKa = 4.49TIDD982 pKa = 3.53SNNKK986 pKa = 9.37YY987 pKa = 9.77GWIWGDD993 pKa = 2.82QGGQFRR999 pKa = 11.84VNANGTVEE1007 pKa = 4.3FRR1009 pKa = 11.84DD1010 pKa = 3.65RR1011 pKa = 11.84DD1012 pKa = 3.69LQFAGLTAGQSITTTINYY1030 pKa = 9.57LIADD1034 pKa = 4.03GDD1036 pKa = 4.11DD1037 pKa = 3.38TDD1039 pKa = 4.07EE1040 pKa = 5.08ASLSLTINGAGTANTAPTGAGILLDD1065 pKa = 4.27PGEE1068 pKa = 5.16DD1069 pKa = 3.46SSASAPHH1076 pKa = 6.16TVTFDD1081 pKa = 3.38SDD1083 pKa = 3.55VTDD1086 pKa = 4.31AEE1088 pKa = 4.51GHH1090 pKa = 4.92TLSYY1094 pKa = 10.9LWDD1097 pKa = 4.63FGDD1100 pKa = 4.09GTTSTLAAPEE1110 pKa = 4.05HH1111 pKa = 6.35TYY1113 pKa = 11.2SVVGFYY1119 pKa = 9.63TVSLTVTDD1127 pKa = 3.8EE1128 pKa = 4.45LGAEE1132 pKa = 4.29TVFDD1136 pKa = 4.14EE1137 pKa = 4.98VSIDD1141 pKa = 3.59VGNAPPVAVADD1152 pKa = 4.07SFTLPVGFDD1161 pKa = 3.25PMANQLNILDD1171 pKa = 4.44NDD1173 pKa = 3.84YY1174 pKa = 11.65DD1175 pKa = 4.41PDD1177 pKa = 4.46GSLNMQIVEE1186 pKa = 4.31IVSGPSFGTVEE1197 pKa = 4.2ILDD1200 pKa = 4.17AAQEE1204 pKa = 4.02LSDD1207 pKa = 3.95RR1208 pKa = 11.84GLPAGQYY1215 pKa = 9.6GQAEE1219 pKa = 4.43YY1220 pKa = 9.22TAFDD1224 pKa = 4.6PNFEE1228 pKa = 4.29GTDD1231 pKa = 3.23SFTYY1235 pKa = 10.08RR1236 pKa = 11.84VQDD1239 pKa = 4.09NEE1241 pKa = 4.42GMWSNIVTAEE1251 pKa = 3.85IVVQNAPVLTLDD1263 pKa = 4.81AVDD1266 pKa = 4.66DD1267 pKa = 5.07GYY1269 pKa = 11.97AVLVADD1275 pKa = 4.48IASGSWVQIQGNIFDD1290 pKa = 5.09NDD1292 pKa = 3.72TSDD1295 pKa = 3.45GALNLLQLDD1304 pKa = 3.92GFKK1307 pKa = 10.9PFDD1310 pKa = 3.86VPDD1313 pKa = 3.63STTSFDD1319 pKa = 3.37SQGRR1323 pKa = 11.84LKK1325 pKa = 10.26IRR1327 pKa = 11.84ADD1329 pKa = 2.97NGGFFRR1335 pKa = 11.84IGSDD1339 pKa = 3.27GSIEE1343 pKa = 4.15FKK1345 pKa = 11.03DD1346 pKa = 3.45RR1347 pKa = 11.84DD1348 pKa = 3.5FDD1350 pKa = 4.03FTGLSSSEE1358 pKa = 4.27SAVTTISYY1366 pKa = 8.85TIGDD1370 pKa = 4.0GTDD1373 pKa = 2.78TDD1375 pKa = 3.91AAFISLTINGSGNVNTAPTGAGILLDD1401 pKa = 4.45PGEE1404 pKa = 5.24DD1405 pKa = 3.5SATSAPHH1412 pKa = 5.85TVTFDD1417 pKa = 3.38SDD1419 pKa = 3.55VTDD1422 pKa = 4.31AEE1424 pKa = 4.51GHH1426 pKa = 4.92TLSYY1430 pKa = 10.9LWDD1433 pKa = 4.63FGDD1436 pKa = 4.09GTTSTLAAPEE1446 pKa = 4.01HH1447 pKa = 6.5TYY1449 pKa = 11.53NSIGFYY1455 pKa = 10.33NVSLTVTDD1463 pKa = 3.91EE1464 pKa = 4.29LGAATVFNEE1473 pKa = 3.69VSIDD1477 pKa = 3.6VGNAPPVAVADD1488 pKa = 4.07SFTLPVGFDD1497 pKa = 3.25PVANHH1502 pKa = 6.61LHH1504 pKa = 6.47ILDD1507 pKa = 4.24NDD1509 pKa = 3.77YY1510 pKa = 11.66DD1511 pKa = 4.51PDD1513 pKa = 4.43GSLNMQVVEE1522 pKa = 4.4IVSGPSFGTVEE1533 pKa = 4.15MMDD1536 pKa = 3.89TAQEE1540 pKa = 3.95LADD1543 pKa = 4.09RR1544 pKa = 11.84GLPAGHH1550 pKa = 6.59YY1551 pKa = 9.77GHH1553 pKa = 7.49AEE1555 pKa = 3.93YY1556 pKa = 8.51ATFDD1560 pKa = 4.19PNFEE1564 pKa = 4.26GTDD1567 pKa = 3.23SFTYY1571 pKa = 10.08RR1572 pKa = 11.84VQDD1575 pKa = 4.9NEE1577 pKa = 4.61GTWSNIVTAEE1587 pKa = 4.14INVQSAPSTPGGALASVNSGSGLDD1611 pKa = 3.42IQDD1614 pKa = 3.13ISVAGDD1620 pKa = 3.16FTVEE1624 pKa = 3.61FWIAFNPGTNTNKK1637 pKa = 9.98YY1638 pKa = 10.18DD1639 pKa = 3.35SAFAGGVYY1647 pKa = 10.36DD1648 pKa = 5.06DD1649 pKa = 5.07TPGDD1653 pKa = 3.94DD1654 pKa = 3.91PSTDD1658 pKa = 3.15GVFGNDD1664 pKa = 3.24FNFHH1668 pKa = 6.56AGLARR1673 pKa = 11.84LYY1675 pKa = 9.66STEE1678 pKa = 4.03IPSGGDD1684 pKa = 3.08AIVANTALTSNGVANHH1700 pKa = 5.81VALVRR1705 pKa = 11.84EE1706 pKa = 4.52GNNFSIYY1713 pKa = 9.8MNGVLDD1719 pKa = 4.0STSTLALDD1727 pKa = 3.78GDD1729 pKa = 4.35LLIEE1733 pKa = 4.15RR1734 pKa = 11.84LATNVRR1740 pKa = 11.84ATKK1743 pKa = 10.38ALSGTLDD1750 pKa = 3.61EE1751 pKa = 5.02FRR1753 pKa = 11.84VWDD1756 pKa = 4.08DD1757 pKa = 3.25ARR1759 pKa = 11.84SASEE1763 pKa = 3.29IATYY1767 pKa = 9.84MNQEE1771 pKa = 4.12VASNADD1777 pKa = 3.34DD1778 pKa = 4.02LLRR1781 pKa = 11.84YY1782 pKa = 9.17YY1783 pKa = 10.78QFDD1786 pKa = 4.42DD1787 pKa = 3.59DD1788 pKa = 4.58TFVIDD1793 pKa = 3.44STGNAEE1799 pKa = 4.05AAGDD1803 pKa = 3.85LSYY1806 pKa = 11.72ASISGVDD1813 pKa = 3.03WFEE1816 pKa = 4.01FSDD1819 pKa = 3.46WLGAA1823 pKa = 3.78

Molecular weight:
194.8 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A254QLH7|A0A254QLH7_9RHOB Sorbitol dehydrogenase OS=Phaeobacter sp. 22II1-1F12B OX=1317111 GN=ATO1_20225 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.43EE41 pKa = 3.72LSAA44 pKa = 5.03

Molecular weight:
5.18 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4790

0

4790

1505669

31

2868

314.3

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.513 ± 0.05

0.899 ± 0.012

6.084 ± 0.034

6.294 ± 0.035

3.795 ± 0.023

8.573 ± 0.036

2.012 ± 0.02

5.365 ± 0.026

3.342 ± 0.026

9.968 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.017

2.747 ± 0.017

5.012 ± 0.024

3.181 ± 0.018

6.551 ± 0.036

5.529 ± 0.022

5.378 ± 0.02

7.166 ± 0.028

1.392 ± 0.015

2.312 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski