Sewage-associated circular DNA virus-30

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI52|A0A0B4UI52_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-30 OX=1592097 PE=4 SV=1
MM1 pKa = 7.51SNFRR5 pKa = 11.84FHH7 pKa = 7.08SKK9 pKa = 10.52SIFLTFPQCDD19 pKa = 3.56YY20 pKa = 10.7PLKK23 pKa = 10.51NFKK26 pKa = 10.91DD27 pKa = 3.52NIEE30 pKa = 4.44AYY32 pKa = 10.55FGDD35 pKa = 3.98NLEE38 pKa = 4.59KK39 pKa = 10.88GVVSQEE45 pKa = 3.42NHH47 pKa = 4.99QDD49 pKa = 3.66GNKK52 pKa = 9.34HH53 pKa = 4.81LHH55 pKa = 6.13AAICLHH61 pKa = 5.15QQVTSRR67 pKa = 11.84DD68 pKa = 3.31PKK70 pKa = 11.13LFDD73 pKa = 4.76KK74 pKa = 11.15LVDD77 pKa = 3.81PAKK80 pKa = 10.34HH81 pKa = 5.81PNIAGRR87 pKa = 11.84FTGGMLKK94 pKa = 10.68AFDD97 pKa = 3.88YY98 pKa = 11.29VMKK101 pKa = 10.37EE102 pKa = 4.15GNFLPLNEE110 pKa = 4.74KK111 pKa = 10.77SFDD114 pKa = 3.56LKK116 pKa = 11.18EE117 pKa = 3.8FLKK120 pKa = 10.9LSKK123 pKa = 10.2EE124 pKa = 4.3KK125 pKa = 10.7KK126 pKa = 9.28NSRR129 pKa = 11.84ASLIVKK135 pKa = 9.46EE136 pKa = 4.41FDD138 pKa = 3.29EE139 pKa = 5.23LPPEE143 pKa = 5.01DD144 pKa = 4.29VMEE147 pKa = 4.36NNKK150 pKa = 10.42DD151 pKa = 3.37FMLLHH156 pKa = 6.7GKK158 pKa = 8.9QMQAYY163 pKa = 8.18VDD165 pKa = 3.31WRR167 pKa = 11.84EE168 pKa = 3.66EE169 pKa = 3.78RR170 pKa = 11.84EE171 pKa = 4.06RR172 pKa = 11.84RR173 pKa = 11.84SKK175 pKa = 10.48FARR178 pKa = 11.84AQAQKK183 pKa = 10.63VFVVPAPGYY192 pKa = 9.6FNAWNNEE199 pKa = 3.54IASWLTTNLRR209 pKa = 11.84QKK211 pKa = 10.21RR212 pKa = 11.84KK213 pKa = 9.43HH214 pKa = 5.94RR215 pKa = 11.84QTQLWIQGPPGIGKK229 pKa = 8.2TSMMMMLEE237 pKa = 4.1EE238 pKa = 5.11LYY240 pKa = 11.1SLTIYY245 pKa = 9.94HH246 pKa = 6.47WPKK249 pKa = 10.91DD250 pKa = 3.85EE251 pKa = 4.51RR252 pKa = 11.84WWDD255 pKa = 3.64LYY257 pKa = 11.74GDD259 pKa = 3.9GQYY262 pKa = 11.47DD263 pKa = 4.15VILLDD268 pKa = 3.62EE269 pKa = 4.71FRR271 pKa = 11.84SQKK274 pKa = 10.15MITEE278 pKa = 4.71LNPILSGDD286 pKa = 4.16PISLSRR292 pKa = 11.84RR293 pKa = 11.84GMAPIVKK300 pKa = 9.38RR301 pKa = 11.84DD302 pKa = 3.83NLPVIIMSNYY312 pKa = 9.7LPEE315 pKa = 4.34EE316 pKa = 4.34CFPTANEE323 pKa = 4.1HH324 pKa = 6.66GKK326 pKa = 10.41LEE328 pKa = 4.25PLLDD332 pKa = 4.64RR333 pKa = 11.84IKK335 pKa = 10.78VVKK338 pKa = 10.56CEE340 pKa = 3.81GPIRR344 pKa = 11.84IVEE347 pKa = 4.17GAPPAEE353 pKa = 4.66CSTPFLFDD361 pKa = 4.32DD362 pKa = 4.67QPPPPPGSQFVLTNDD377 pKa = 3.16YY378 pKa = 11.48SEE380 pKa = 5.47IFPQTPPEE388 pKa = 4.28YY389 pKa = 9.84EE390 pKa = 4.29TIDD393 pKa = 5.45DD394 pKa = 5.58DD395 pKa = 4.7DD396 pKa = 4.45LQPGRR401 pKa = 11.84VCNARR406 pKa = 11.84PQQSTYY412 pKa = 11.22LHH414 pKa = 6.88IFPEE418 pKa = 3.91NRR420 pKa = 11.84EE421 pKa = 3.77AYY423 pKa = 9.72ARR425 pKa = 11.84YY426 pKa = 9.38HH427 pKa = 6.46GDD429 pKa = 3.35AAVVPYY435 pKa = 9.81TSADD439 pKa = 3.3FDD441 pKa = 4.82RR442 pKa = 11.84NNDD445 pKa = 3.0PFYY448 pKa = 10.56FDD450 pKa = 4.06KK451 pKa = 10.73ISRR454 pKa = 11.84QSRR457 pKa = 11.84AAMLAARR464 pKa = 11.84NII466 pKa = 3.75

Molecular weight: 53.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI52|A0A0B4UI52_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-30 OX=1592097 PE=4 SV=1
MM1 pKa = 7.97AYY3 pKa = 9.58PRR5 pKa = 11.84GRR7 pKa = 11.84RR8 pKa = 11.84TPLKK12 pKa = 10.33RR13 pKa = 11.84RR14 pKa = 11.84ATYY17 pKa = 10.66GRR19 pKa = 11.84GTRR22 pKa = 11.84TFARR26 pKa = 11.84TKK28 pKa = 10.19FRR30 pKa = 11.84RR31 pKa = 11.84AYY33 pKa = 8.21FNRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GVGRR42 pKa = 11.84KK43 pKa = 7.48EE44 pKa = 4.02KK45 pKa = 10.37KK46 pKa = 10.08GCDD49 pKa = 3.39VSFATAGIIASTSTNGGIYY68 pKa = 10.02LLNGIQEE75 pKa = 5.07GVGSWNRR82 pKa = 11.84IGRR85 pKa = 11.84YY86 pKa = 6.34MWNKK90 pKa = 10.31SIEE93 pKa = 4.19LDD95 pKa = 3.17LTLRR99 pKa = 11.84YY100 pKa = 7.76TSSYY104 pKa = 10.13GALDD108 pKa = 3.43TDD110 pKa = 3.62ITSGEE115 pKa = 4.04WVRR118 pKa = 11.84AVLVWDD124 pKa = 3.8KK125 pKa = 11.02QPNGGTIPTFDD136 pKa = 4.05TIFGQTSQAGVEE148 pKa = 4.43SASVMDD154 pKa = 4.1HH155 pKa = 6.38LRR157 pKa = 11.84YY158 pKa = 10.85DD159 pKa = 3.03NMFRR163 pKa = 11.84FKK165 pKa = 11.26VLMDD169 pKa = 3.29EE170 pKa = 5.3CINPTLTNAAVSTPINPASASLVNNTYY197 pKa = 10.11FRR199 pKa = 11.84FHH201 pKa = 7.19KK202 pKa = 10.24YY203 pKa = 9.41VKK205 pKa = 9.82LRR207 pKa = 11.84NMPTNFSGTANPMTTANISTGALYY231 pKa = 10.76LILRR235 pKa = 11.84TQSATLTEE243 pKa = 4.48LWGHH247 pKa = 6.51

Molecular weight: 27.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

713

247

466

356.5

40.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.592 ± 0.585

1.122 ± 0.166

5.891 ± 1.194

5.47 ± 1.401

5.33 ± 0.466

6.171 ± 1.454

2.104 ± 0.473

5.189 ± 0.039

5.75 ± 0.905

7.994 ± 0.376

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.945 ± 0.059

5.891 ± 0.312

6.452 ± 1.493

3.787 ± 0.937

7.013 ± 1.222

5.891 ± 0.527

6.171 ± 2.746

4.628 ± 0.122

1.683 ± 0.181

3.927 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski