Mycobacterium phage MalagasyRose

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TEA8|A0A5J6TEA8_9CAUD Uncharacterized protein OS=Mycobacterium phage MalagasyRose OX=2599870 GN=42 PE=4 SV=1
MM1 pKa = 7.94CDD3 pKa = 3.72LGPFCSHH10 pKa = 7.25RR11 pKa = 11.84GGDD14 pKa = 3.18RR15 pKa = 11.84GKK17 pKa = 10.52RR18 pKa = 11.84EE19 pKa = 3.94PSDD22 pKa = 3.77SDD24 pKa = 4.07GLGCLEE30 pKa = 3.76NVYY33 pKa = 9.93HH34 pKa = 7.07HH35 pKa = 7.15EE36 pKa = 4.26DD37 pKa = 4.02AEE39 pKa = 4.43PEE41 pKa = 4.01SDD43 pKa = 3.49PVQLAGDD50 pKa = 4.46RR51 pKa = 11.84LPLSRR56 pKa = 11.84SWTLDD61 pKa = 3.06EE62 pKa = 5.58SGSTGCATCDD72 pKa = 3.1GGGCRR77 pKa = 11.84DD78 pKa = 4.73CIDD81 pKa = 3.12

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TDF3|A0A5J6TDF3_9CAUD Terminase small subunit OS=Mycobacterium phage MalagasyRose OX=2599870 GN=6 PE=4 SV=1
MM1 pKa = 7.29SRR3 pKa = 11.84YY4 pKa = 9.32GQGGSRR10 pKa = 11.84ALRR13 pKa = 11.84EE14 pKa = 3.99FNAGILARR22 pKa = 11.84DD23 pKa = 3.54GHH25 pKa = 5.76RR26 pKa = 11.84CRR28 pKa = 11.84LQIGGVCIGHH38 pKa = 6.78ATQVDD43 pKa = 4.23HH44 pKa = 6.52KK45 pKa = 9.65TNLAKK50 pKa = 10.62LRR52 pKa = 11.84LNRR55 pKa = 11.84NDD57 pKa = 3.26PRR59 pKa = 11.84AMNPRR64 pKa = 11.84NAQAACEE71 pKa = 4.11PCHH74 pKa = 5.2EE75 pKa = 4.46WKK77 pKa = 10.45SEE79 pKa = 3.97RR80 pKa = 11.84EE81 pKa = 3.97RR82 pKa = 11.84IAALAEE88 pKa = 4.17VNKK91 pKa = 10.26ARR93 pKa = 11.84AAARR97 pKa = 11.84RR98 pKa = 11.84ARR100 pKa = 11.84LRR102 pKa = 11.84RR103 pKa = 11.84PVEE106 pKa = 3.87PHH108 pKa = 6.42PGDD111 pKa = 3.62YY112 pKa = 11.08

Molecular weight: 12.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

16997

37

1000

202.3

21.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.449 ± 0.411

1.124 ± 0.126

6.654 ± 0.204

5.43 ± 0.301

2.665 ± 0.167

8.949 ± 0.498

2.324 ± 0.157

3.565 ± 0.187

2.818 ± 0.193

8.407 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.141

2.742 ± 0.158

6.283 ± 0.253

3.512 ± 0.168

7.19 ± 0.386

4.901 ± 0.245

6.201 ± 0.229

8.066 ± 0.271

2.206 ± 0.159

2.453 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski