Peptoniphilus duerdenii ATCC BAA-1640

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; Peptoniphilus duerdenii

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1987 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0NLD6|E0NLD6_9FIRM Uncharacterized protein OS=Peptoniphilus duerdenii ATCC BAA-1640 OX=862517 GN=HMPREF9225_0975 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.16KK3 pKa = 10.19VYY5 pKa = 10.42HH6 pKa = 6.69EE7 pKa = 4.92DD8 pKa = 3.52CGCGCGCGHH17 pKa = 7.18DD18 pKa = 4.53HH19 pKa = 7.61DD20 pKa = 5.67HH21 pKa = 7.61DD22 pKa = 4.77HH23 pKa = 7.38DD24 pKa = 4.57LEE26 pKa = 4.77NEE28 pKa = 4.28EE29 pKa = 4.54IEE31 pKa = 4.61TMVLTLEE38 pKa = 5.2DD39 pKa = 4.15DD40 pKa = 4.17TEE42 pKa = 4.61LEE44 pKa = 4.29CQALGVFEE52 pKa = 5.11FEE54 pKa = 4.09GNEE57 pKa = 4.12YY58 pKa = 10.04IALVPMSGEE67 pKa = 4.09LEE69 pKa = 4.02DD70 pKa = 4.51EE71 pKa = 4.66IIVFGYY77 pKa = 10.74EE78 pKa = 3.8EE79 pKa = 5.18LEE81 pKa = 4.3DD82 pKa = 5.31DD83 pKa = 4.94EE84 pKa = 5.38VALHH88 pKa = 6.08QIEE91 pKa = 4.24SDD93 pKa = 3.51EE94 pKa = 4.21EE95 pKa = 4.24YY96 pKa = 11.2EE97 pKa = 5.02RR98 pKa = 11.84ILDD101 pKa = 3.79FLDD104 pKa = 4.26TIIEE108 pKa = 4.12EE109 pKa = 4.53EE110 pKa = 4.17EE111 pKa = 4.21DD112 pKa = 3.65EE113 pKa = 4.4EE114 pKa = 4.65

Molecular weight: 13.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0NJL0|E0NJL0_9FIRM Transcriptional regulator TetR family OS=Peptoniphilus duerdenii ATCC BAA-1640 OX=862517 GN=HMPREF9225_0349 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.31NKK10 pKa = 8.92QRR12 pKa = 11.84KK13 pKa = 7.85RR14 pKa = 11.84EE15 pKa = 3.9HH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.14SGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.31GRR39 pKa = 11.84KK40 pKa = 8.86KK41 pKa = 10.58LSAA44 pKa = 3.95

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1987

0

1987

618953

32

5170

311.5

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.599 ± 0.065

0.827 ± 0.021

6.165 ± 0.054

8.08 ± 0.066

4.618 ± 0.042

6.403 ± 0.054

1.351 ± 0.02

9.096 ± 0.079

9.189 ± 0.071

9.147 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.669 ± 0.039

5.618 ± 0.052

2.859 ± 0.041

2.126 ± 0.029

3.857 ± 0.045

6.205 ± 0.053

4.996 ± 0.072

6.464 ± 0.055

0.602 ± 0.017

4.128 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski