Mycoplasma sp. CAG:877

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; environmental samples

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1407 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5LIU2|R5LIU2_9MOLU ABC-type multidrug transport system ATPase and permease component OS=Mycoplasma sp. CAG:877 OX=1262907 GN=BN801_00388 PE=3 SV=1
MM1 pKa = 7.42ISSIKK6 pKa = 10.61NYY8 pKa = 9.97IINFFYY14 pKa = 10.92KK15 pKa = 10.32LKK17 pKa = 10.1TVVDD21 pKa = 4.04VDD23 pKa = 3.7DD24 pKa = 5.2DD25 pKa = 4.66FYY27 pKa = 11.91DD28 pKa = 3.66EE29 pKa = 4.72VIYY32 pKa = 10.8LIDD35 pKa = 4.37LEE37 pKa = 4.6VV38 pKa = 3.46

Molecular weight: 4.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5LI61|R5LI61_9MOLU Uncharacterized protein OS=Mycoplasma sp. CAG:877 OX=1262907 GN=BN801_00110 PE=4 SV=1
MM1 pKa = 7.95AEE3 pKa = 3.85FNRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 10.35KK9 pKa = 9.49KK10 pKa = 10.01VCMMCTKK17 pKa = 10.48NAVVDD22 pKa = 4.03YY23 pKa = 10.47KK24 pKa = 11.22DD25 pKa = 3.71PEE27 pKa = 4.05TLRR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 8.15TNEE35 pKa = 3.44KK36 pKa = 9.73GKK38 pKa = 10.17IVPRR42 pKa = 11.84RR43 pKa = 11.84VTGNCALHH51 pKa = 5.57QRR53 pKa = 11.84YY54 pKa = 8.4IAKK57 pKa = 9.17QVKK60 pKa = 8.93RR61 pKa = 11.84ARR63 pKa = 11.84AIALMPYY70 pKa = 8.81TRR72 pKa = 4.6

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1407

0

1407

417977

31

2833

297.1

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.627 ± 0.059

1.109 ± 0.027

6.183 ± 0.06

7.262 ± 0.079

4.328 ± 0.053

5.521 ± 0.073

1.27 ± 0.022

9.289 ± 0.08

9.387 ± 0.079

9.078 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.029

6.803 ± 0.079

2.594 ± 0.037

2.21 ± 0.039

3.351 ± 0.049

6.587 ± 0.066

5.807 ± 0.076

6.676 ± 0.068

0.553 ± 0.014

5.076 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski