Faba bean necrotic stunt virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Nanovirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7DLN5|C7DLN5_9VIRU Movement protein OS=Faba bean necrotic stunt virus OX=283824 PE=4 SV=1
MM1 pKa = 7.0GLKK4 pKa = 9.93YY5 pKa = 10.42FSVLPVEE12 pKa = 4.25LRR14 pKa = 11.84EE15 pKa = 4.35KK16 pKa = 10.28IVRR19 pKa = 11.84DD20 pKa = 3.35HH21 pKa = 6.85LRR23 pKa = 11.84EE24 pKa = 3.84EE25 pKa = 4.19RR26 pKa = 11.84KK27 pKa = 9.89KK28 pKa = 10.98EE29 pKa = 3.75FLEE32 pKa = 3.97NAIEE36 pKa = 4.27DD37 pKa = 3.65SCRR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 5.48QSLITEE48 pKa = 4.64DD49 pKa = 4.37PSTEE53 pKa = 4.28DD54 pKa = 4.7LLSLCKK60 pKa = 10.16FLEE63 pKa = 4.11SLAYY67 pKa = 10.34YY68 pKa = 10.23VGNQFNTRR76 pKa = 11.84CLIKK80 pKa = 9.65WRR82 pKa = 11.84KK83 pKa = 9.08DD84 pKa = 3.02VPFQIKK90 pKa = 9.55YY91 pKa = 10.5GVMEE95 pKa = 4.1EE96 pKa = 4.01QHH98 pKa = 5.92IKK100 pKa = 10.74LYY102 pKa = 10.96GILDD106 pKa = 3.99MEE108 pKa = 4.84DD109 pKa = 3.37LACRR113 pKa = 11.84EE114 pKa = 3.95LLIPEE119 pKa = 4.48EE120 pKa = 4.42EE121 pKa = 4.39DD122 pKa = 4.53DD123 pKa = 3.34ITYY126 pKa = 10.81EE127 pKa = 4.33DD128 pKa = 5.17GIIVNCKK135 pKa = 9.8QLDD138 pKa = 3.64NLFAEE143 pKa = 4.72LGINVVYY150 pKa = 9.37ITVSKK155 pKa = 11.04NCIHH159 pKa = 6.46TPLNKK164 pKa = 10.28EE165 pKa = 3.74IVINN169 pKa = 3.98

Molecular weight: 19.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7DLN4|C7DLN4_9VIRU Cell-cycle link protein OS=Faba bean necrotic stunt virus OX=283824 GN=clink PE=4 SV=1
MM1 pKa = 7.0VSNWNWSGKK10 pKa = 9.44KK11 pKa = 10.0GRR13 pKa = 11.84RR14 pKa = 11.84TPRR17 pKa = 11.84RR18 pKa = 11.84GYY20 pKa = 8.4TRR22 pKa = 11.84PFKK25 pKa = 10.89SAVPTTRR32 pKa = 11.84VVVHH36 pKa = 6.05QSAVLKK42 pKa = 10.51KK43 pKa = 10.15DD44 pKa = 3.49DD45 pKa = 3.87VSGSEE50 pKa = 4.29IKK52 pKa = 10.68PEE54 pKa = 4.03GDD56 pKa = 2.94VARR59 pKa = 11.84YY60 pKa = 9.15KK61 pKa = 10.12IRR63 pKa = 11.84KK64 pKa = 8.78VMLSCTLRR72 pKa = 11.84MRR74 pKa = 11.84PGEE77 pKa = 4.05LVNYY81 pKa = 9.31LIVKK85 pKa = 9.75CSSPIVNWSAAFTAPALMVKK105 pKa = 9.98EE106 pKa = 4.59SCQDD110 pKa = 3.28MITIIGKK117 pKa = 10.25GKK119 pKa = 10.06VEE121 pKa = 4.64SNGVAGSDD129 pKa = 3.65CTKK132 pKa = 10.72SFNKK136 pKa = 10.25FIRR139 pKa = 11.84LGAGISQTQHH149 pKa = 6.9LYY151 pKa = 9.66VVMYY155 pKa = 9.43TSEE158 pKa = 3.97AVKK161 pKa = 9.12TVLEE165 pKa = 3.96HH166 pKa = 5.98RR167 pKa = 11.84VYY169 pKa = 10.76IEE171 pKa = 3.67VV172 pKa = 3.31

Molecular weight: 19.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1282

106

286

160.3

18.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.368 ± 0.898

2.964 ± 0.564

5.616 ± 0.708

7.41 ± 1.155

3.9 ± 0.531

5.46 ± 0.845

1.872 ± 0.14

6.63 ± 0.76

6.552 ± 0.65

9.282 ± 1.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.12 ± 0.368

4.914 ± 0.588

3.042 ± 0.264

3.198 ± 0.357

6.474 ± 0.438

7.488 ± 0.854

4.29 ± 0.501

7.332 ± 0.963

1.326 ± 0.254

4.758 ± 0.491
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski