Wenzhou Crab Virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Nyamiviridae; Crustavirus; Wenzhou crustavirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0B5KTI5|A0A0B5KTI5_9MONO Nucleocapsid OS=Wenzhou Crab Virus 1 OX=1608091 GN=N PE=4 SV=1
MM1 pKa = 7.57FEE3 pKa = 4.23EE4 pKa = 4.82PPVVEE9 pKa = 4.18EE10 pKa = 4.0EE11 pKa = 4.29KK12 pKa = 11.19EE13 pKa = 3.98EE14 pKa = 3.96FVYY17 pKa = 10.46RR18 pKa = 11.84RR19 pKa = 11.84QTRR22 pKa = 11.84VTAAITVSHH31 pKa = 6.63LEE33 pKa = 3.64SWEE36 pKa = 4.11NGFCVPDD43 pKa = 3.75LSMFLEE49 pKa = 4.3KK50 pKa = 10.74EE51 pKa = 4.07GFKK54 pKa = 10.65RR55 pKa = 11.84GGPALSDD62 pKa = 3.1TRR64 pKa = 11.84AYY66 pKa = 10.72AQFLYY71 pKa = 8.47TTFWKK76 pKa = 10.39NQTSLKK82 pKa = 10.15PLDD85 pKa = 3.86RR86 pKa = 11.84GILSTAKK93 pKa = 10.53DD94 pKa = 3.65LTQLGSLNWANCDD107 pKa = 3.27FSLPITDD114 pKa = 5.24LYY116 pKa = 11.03SEE118 pKa = 5.01HH119 pKa = 7.24IISKK123 pKa = 10.22LNGIRR128 pKa = 11.84APWDD132 pKa = 3.19KK133 pKa = 10.61MIQTLSNSTNRR144 pKa = 11.84VCHH147 pKa = 5.47YY148 pKa = 9.33EE149 pKa = 3.55IKK151 pKa = 10.23QFKK154 pKa = 8.06TQFHH158 pKa = 7.2FYY160 pKa = 11.07GDD162 pKa = 4.02YY163 pKa = 10.99CVVTHH168 pKa = 7.03RR169 pKa = 11.84GKK171 pKa = 10.56KK172 pKa = 10.06VFMTWLMLLAFQDD185 pKa = 4.25SIIGLMDD192 pKa = 3.54CCMFSFLHH200 pKa = 6.05EE201 pKa = 4.44NEE203 pKa = 4.51SGVPLYY209 pKa = 10.66QDD211 pKa = 3.44VAQMVVDD218 pKa = 5.44GIEE221 pKa = 4.35TICNLGEE228 pKa = 3.63EE229 pKa = 4.74AYY231 pKa = 10.44DD232 pKa = 3.55VFKK235 pKa = 10.83ALHH238 pKa = 6.43PGAVGRR244 pKa = 11.84ILLLEE249 pKa = 4.39SIYY252 pKa = 10.72TNPLFEE258 pKa = 4.47EE259 pKa = 4.81TVKK262 pKa = 11.01DD263 pKa = 4.38MPDD266 pKa = 3.45CPIKK270 pKa = 11.24VMLQAPLEE278 pKa = 4.58DD279 pKa = 4.94DD280 pKa = 3.87LAHH283 pKa = 6.35MKK285 pKa = 10.67LLTLCGMAKK294 pKa = 10.3CFTYY298 pKa = 9.98PVIYY302 pKa = 10.09IQEE305 pKa = 4.23SVDD308 pKa = 3.47NVTSKK313 pKa = 11.04SGVFNPDD320 pKa = 2.7RR321 pKa = 11.84NGGNMSLWYY330 pKa = 9.95FRR332 pKa = 11.84RR333 pKa = 11.84TFCYY337 pKa = 10.31NYY339 pKa = 10.25LRR341 pKa = 11.84RR342 pKa = 11.84HH343 pKa = 5.92KK344 pKa = 9.29EE345 pKa = 3.77WPPLTITGPIRR356 pKa = 11.84GDD358 pKa = 3.57LSRR361 pKa = 11.84SIASGAWAEE370 pKa = 3.9PRR372 pKa = 11.84NNKK375 pKa = 8.58WSLLEE380 pKa = 3.94FKK382 pKa = 10.71NIEE385 pKa = 3.97LQKK388 pKa = 10.86ILDD391 pKa = 4.1LNLKK395 pKa = 9.89VDD397 pKa = 3.92EE398 pKa = 4.82TDD400 pKa = 3.71LLSDD404 pKa = 3.36KK405 pKa = 10.87AIAVGLDD412 pKa = 3.1SWPRR416 pKa = 11.84EE417 pKa = 3.61YY418 pKa = 10.91DD419 pKa = 3.11RR420 pKa = 11.84RR421 pKa = 11.84YY422 pKa = 10.05HH423 pKa = 6.35RR424 pKa = 11.84LLYY427 pKa = 10.38GYY429 pKa = 9.18SANPGPIKK437 pKa = 10.35NRR439 pKa = 11.84RR440 pKa = 11.84LLIEE444 pKa = 4.34HH445 pKa = 7.45IKK447 pKa = 10.52RR448 pKa = 11.84EE449 pKa = 4.27EE450 pKa = 3.8VSVFYY455 pKa = 10.89DD456 pKa = 3.09ATSFLKK462 pKa = 10.91GDD464 pKa = 4.02DD465 pKa = 4.12KK466 pKa = 10.37TSKK469 pKa = 9.31MAVMCLKK476 pKa = 10.29EE477 pKa = 4.31RR478 pKa = 11.84EE479 pKa = 4.24LNRR482 pKa = 11.84KK483 pKa = 8.95KK484 pKa = 11.0GRR486 pKa = 11.84FFTKK490 pKa = 8.6QTMKK494 pKa = 10.5GRR496 pKa = 11.84QYY498 pKa = 11.46QLLLEE503 pKa = 4.63EE504 pKa = 4.14NMKK507 pKa = 10.41PILSYY512 pKa = 11.46VPLTSMILSGDD523 pKa = 3.86SLLKK527 pKa = 10.58YY528 pKa = 10.3CLNQSADD535 pKa = 3.53PNTAKK540 pKa = 10.62VSVDD544 pKa = 3.52FSKK547 pKa = 9.25WCQYY551 pKa = 8.58QRR553 pKa = 11.84QGLIGPIASEE563 pKa = 3.61IDD565 pKa = 3.57RR566 pKa = 11.84IYY568 pKa = 10.85GSSPIYY574 pKa = 10.22EE575 pKa = 4.5CAHH578 pKa = 6.62LVPLDD583 pKa = 3.76LWWFFQDD590 pKa = 4.09SSRR593 pKa = 11.84PPQQSADD600 pKa = 3.83GEE602 pKa = 4.39PCAGDD607 pKa = 3.2RR608 pKa = 11.84AFPGLHH614 pKa = 6.15SMGEE618 pKa = 4.01GMYY621 pKa = 10.28QKK623 pKa = 10.84LWTLVTGCGIMAQMYY638 pKa = 9.67KK639 pKa = 10.56RR640 pKa = 11.84NLEE643 pKa = 4.08CGIMGSGDD651 pKa = 3.36NQYY654 pKa = 10.53ISCKK658 pKa = 9.62IPNDD662 pKa = 3.32MGPRR666 pKa = 11.84EE667 pKa = 4.03VQRR670 pKa = 11.84AIFEE674 pKa = 4.21GLEE677 pKa = 4.12EE678 pKa = 4.19FSSICKK684 pKa = 10.31LPIKK688 pKa = 10.54LEE690 pKa = 3.99EE691 pKa = 4.28TFVSSHH697 pKa = 4.64YY698 pKa = 9.56MEE700 pKa = 4.91YY701 pKa = 11.18GRR703 pKa = 11.84TSYY706 pKa = 11.07LRR708 pKa = 11.84GKK710 pKa = 10.29RR711 pKa = 11.84IPQALKK717 pKa = 8.31RR718 pKa = 11.84ASRR721 pKa = 11.84IGTEE725 pKa = 4.07SQEE728 pKa = 4.47TIPSVNTKK736 pKa = 10.43LSGVFATGIATASEE750 pKa = 4.67CGTPASAYY758 pKa = 9.39AVTIIEE764 pKa = 4.23SCLALEE770 pKa = 4.37ARR772 pKa = 11.84GFQGGPTRR780 pKa = 11.84MGALLILGRR789 pKa = 11.84QLGGVKK795 pKa = 10.31SSPYY799 pKa = 10.39AAFCIRR805 pKa = 11.84GLNDD809 pKa = 3.15TATVGVSVLQTLIKK823 pKa = 10.89YY824 pKa = 9.78KK825 pKa = 10.95DD826 pKa = 3.44SFGKK830 pKa = 10.65LFLSLKK836 pKa = 8.27TLPIFLGRR844 pKa = 11.84VNWGQMFKK852 pKa = 10.95DD853 pKa = 4.67PYY855 pKa = 9.58SLPYY859 pKa = 10.27LRR861 pKa = 11.84PRR863 pKa = 11.84DD864 pKa = 3.8ADD866 pKa = 3.7SVIRR870 pKa = 11.84DD871 pKa = 3.63IVKK874 pKa = 8.32EE875 pKa = 3.99TLPEE879 pKa = 3.65EE880 pKa = 4.31TKK882 pKa = 10.75NVKK885 pKa = 10.28LSALLGAHH893 pKa = 7.2AEE895 pKa = 4.1AEE897 pKa = 4.27EE898 pKa = 4.14QKK900 pKa = 11.06LIATLASMEE909 pKa = 4.11PMSLKK914 pKa = 10.64LGAGLYY920 pKa = 10.14SLSNVAIKK928 pKa = 10.36EE929 pKa = 4.13KK930 pKa = 10.58ILGQFHH936 pKa = 6.53TSSSVLKK943 pKa = 10.21LGSRR947 pKa = 11.84HH948 pKa = 5.56GYY950 pKa = 8.32KK951 pKa = 10.65DD952 pKa = 3.23KK953 pKa = 11.55VQDD956 pKa = 4.0ALEE959 pKa = 4.67SDD961 pKa = 3.93NSMLAHH967 pKa = 7.09FSTKK971 pKa = 9.55GKK973 pKa = 9.89NCIVPWCEE981 pKa = 3.59KK982 pKa = 10.07HH983 pKa = 7.17DD984 pKa = 4.32CPTHH988 pKa = 7.22IMDD991 pKa = 4.17MVRR994 pKa = 11.84EE995 pKa = 4.33GVVGRR1000 pKa = 11.84SLSNPSSPAPQHH1012 pKa = 5.63QFVLAHH1018 pKa = 6.34WSAVPTHH1025 pKa = 6.46MIRR1028 pKa = 11.84RR1029 pKa = 11.84SIKK1032 pKa = 9.47VVVDD1036 pKa = 3.6DD1037 pKa = 5.2GVNLLHH1043 pKa = 6.34TLRR1046 pKa = 11.84GKK1048 pKa = 9.13RR1049 pKa = 11.84ACYY1052 pKa = 9.52IGSDD1056 pKa = 3.63TGVKK1060 pKa = 9.51RR1061 pKa = 11.84QKK1063 pKa = 10.91SPLALICPDD1072 pKa = 3.77SLDD1075 pKa = 3.53VSAMSLGEE1083 pKa = 3.61IRR1085 pKa = 11.84SWVGGEE1091 pKa = 4.14FNMTSLIDD1099 pKa = 3.71IMIQEE1104 pKa = 4.57KK1105 pKa = 8.85TFSDD1109 pKa = 4.29PATVAAAADD1118 pKa = 4.41TISGGCYY1125 pKa = 6.08QHH1127 pKa = 7.14RR1128 pKa = 11.84GRR1130 pKa = 11.84VPTLSQGAHH1139 pKa = 5.84INYY1142 pKa = 9.86DD1143 pKa = 3.98PVAMSYY1149 pKa = 10.52IRR1151 pKa = 11.84ISTDD1155 pKa = 2.78TALDD1159 pKa = 3.74FAKK1162 pKa = 10.59KK1163 pKa = 10.4GGDD1166 pKa = 3.51YY1167 pKa = 10.85TIMFQSIKK1175 pKa = 9.42VTIASQLLHH1184 pKa = 7.11RR1185 pKa = 11.84LQCGEE1190 pKa = 4.88DD1191 pKa = 3.75ISGEE1195 pKa = 3.65WAAILICNTCTQPVHH1210 pKa = 7.37DD1211 pKa = 4.65GAFDD1215 pKa = 3.9LAHH1218 pKa = 6.49FPSYY1222 pKa = 11.15SGFSLGTEE1230 pKa = 4.14KK1231 pKa = 10.44ILQFRR1236 pKa = 11.84RR1237 pKa = 11.84LGYY1240 pKa = 10.27EE1241 pKa = 3.74YY1242 pKa = 10.81VGPEE1246 pKa = 3.82DD1247 pKa = 3.65VEE1249 pKa = 4.36AVSEE1253 pKa = 4.35GAHH1256 pKa = 5.44VFLAEE1261 pKa = 4.3KK1262 pKa = 10.22LISSAINHH1270 pKa = 6.14DD1271 pKa = 3.95HH1272 pKa = 7.37VYY1274 pKa = 9.79MAMEE1278 pKa = 5.12LDD1280 pKa = 3.9RR1281 pKa = 11.84VTGSDD1286 pKa = 4.22DD1287 pKa = 3.61FALNPQASLTDD1298 pKa = 3.96LARR1301 pKa = 11.84ADD1303 pKa = 3.32IKK1305 pKa = 11.08LLLMCIFQRR1314 pKa = 11.84IAIRR1318 pKa = 11.84PPFKK1322 pKa = 10.75DD1323 pKa = 4.04VIAHH1327 pKa = 6.59LCASVPEE1334 pKa = 4.38HH1335 pKa = 7.22RR1336 pKa = 11.84EE1337 pKa = 3.63TMSPIKK1343 pKa = 10.5LIMDD1347 pKa = 5.05PIIAAGRR1354 pKa = 11.84LSDD1357 pKa = 3.51LHH1359 pKa = 7.6RR1360 pKa = 11.84LAGSYY1365 pKa = 10.32SLPEE1369 pKa = 4.0VEE1371 pKa = 4.92SEE1373 pKa = 4.19RR1374 pKa = 11.84EE1375 pKa = 3.84KK1376 pKa = 11.72VMLFAYY1382 pKa = 9.83LWRR1385 pKa = 11.84RR1386 pKa = 11.84LYY1388 pKa = 10.82CPSIRR1393 pKa = 11.84SWKK1396 pKa = 10.25YY1397 pKa = 8.83FLPDD1401 pKa = 3.15DD1402 pKa = 4.14SAASRR1407 pKa = 11.84VRR1409 pKa = 11.84GLLLKK1414 pKa = 10.89NKK1416 pKa = 9.17ICSGDD1421 pKa = 3.54PTIEE1425 pKa = 3.84GAIRR1429 pKa = 11.84SCCDD1433 pKa = 3.25DD1434 pKa = 3.45STLEE1438 pKa = 4.05TLEE1441 pKa = 4.71LLLDD1445 pKa = 4.01FRR1447 pKa = 11.84PVSNPQDD1454 pKa = 3.53SQRR1457 pKa = 11.84SMRR1460 pKa = 11.84ARR1462 pKa = 11.84EE1463 pKa = 3.95APMQRR1468 pKa = 11.84SSVCKK1473 pKa = 10.11LGHH1476 pKa = 6.17GPQGGAPVQNAALRR1490 pKa = 11.84DD1491 pKa = 3.65LSTGYY1496 pKa = 10.74GNSVLASQIYY1506 pKa = 10.21SIIDD1510 pKa = 3.65TLPDD1514 pKa = 3.4LPVWSIYY1521 pKa = 9.93DD1522 pKa = 3.37HH1523 pKa = 6.66NGAIGVLVGHH1533 pKa = 6.71KK1534 pKa = 7.99RR1535 pKa = 11.84TSYY1538 pKa = 10.21IHH1540 pKa = 7.28DD1541 pKa = 4.3PVPSSSMNIRR1551 pKa = 11.84LSNGPSSLSIDD1562 pKa = 3.72EE1563 pKa = 4.72CSASYY1568 pKa = 9.89SHH1570 pKa = 7.33EE1571 pKa = 4.09YY1572 pKa = 9.92YY1573 pKa = 10.6VQNLGLTAPIRR1584 pKa = 11.84PTRR1587 pKa = 11.84SPLIIICSHH1596 pKa = 5.85GFQNKK1601 pKa = 9.96GITPLDD1607 pKa = 4.4DD1608 pKa = 4.5DD1609 pKa = 4.0WVLTKK1614 pKa = 11.17DD1615 pKa = 3.39MTDD1618 pKa = 2.75GGTLVGCPRR1627 pKa = 11.84RR1628 pKa = 11.84TVGDD1632 pKa = 3.04VWYY1635 pKa = 9.37CNKK1638 pKa = 9.39MPQVDD1643 pKa = 3.9EE1644 pKa = 4.69RR1645 pKa = 11.84GSPNTILGFPTEE1657 pKa = 4.48TRR1659 pKa = 11.84LSPYY1663 pKa = 10.08VRR1665 pKa = 11.84LKK1667 pKa = 11.1EE1668 pKa = 4.66FLDD1671 pKa = 3.83LDD1673 pKa = 4.0NEE1675 pKa = 4.37FTLKK1679 pKa = 10.76DD1680 pKa = 3.61VEE1682 pKa = 4.4QKK1684 pKa = 11.34VNDD1687 pKa = 3.81TLKK1690 pKa = 10.9AIEE1693 pKa = 4.22TGQRR1697 pKa = 11.84EE1698 pKa = 4.36QLSEE1702 pKa = 4.62IIPPLLIQSGADD1714 pKa = 3.2VQTKK1718 pKa = 8.62YY1719 pKa = 10.48RR1720 pKa = 11.84GYY1722 pKa = 10.62GEE1724 pKa = 4.76VMLHH1728 pKa = 6.17LWISQKK1734 pKa = 9.91FRR1736 pKa = 11.84SPSLYY1741 pKa = 7.76TMKK1744 pKa = 9.66WHH1746 pKa = 6.14RR1747 pKa = 11.84HH1748 pKa = 3.55LWMYY1752 pKa = 10.66HH1753 pKa = 6.38AGCACEE1759 pKa = 3.8NVEE1762 pKa = 3.82WAEE1765 pKa = 3.98YY1766 pKa = 9.91KK1767 pKa = 10.75PKK1769 pKa = 9.8DD1770 pKa = 3.53TLLAAFVEE1778 pKa = 4.68SLAYY1782 pKa = 10.18LAASEE1787 pKa = 4.49VVPEE1791 pKa = 4.3DD1792 pKa = 3.81VPVSSIPDD1800 pKa = 3.59LGILGDD1806 pKa = 3.95LL1807 pKa = 4.23

Molecular weight:
202.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0B5KXH5|A0A0B5KXH5_9MONO Glycoprotein OS=Wenzhou Crab Virus 1 OX=1608091 GN=G PE=4 SV=1
MM1 pKa = 7.43APIIVFLLGLSVLDD15 pKa = 3.82PCNAQSGLLCMRR27 pKa = 11.84SHH29 pKa = 7.58PLHH32 pKa = 6.44VYY34 pKa = 9.78KK35 pKa = 10.78LPRR38 pKa = 11.84VSPCRR43 pKa = 11.84RR44 pKa = 11.84KK45 pKa = 9.47IRR47 pKa = 11.84EE48 pKa = 3.93EE49 pKa = 3.82GRR51 pKa = 11.84ITTKK55 pKa = 10.31TPNEE59 pKa = 3.8EE60 pKa = 4.69AYY62 pKa = 9.28VTDD65 pKa = 3.27AWLVSLDD72 pKa = 3.87TCWANSTTGFFGAHH86 pKa = 5.41TCVQGCSEE94 pKa = 4.13QTLDD98 pKa = 3.2RR99 pKa = 11.84HH100 pKa = 5.68EE101 pKa = 4.89ALDD104 pKa = 4.01IKK106 pKa = 11.24NGFCPDD112 pKa = 3.81LGLASHH118 pKa = 6.8TKK120 pKa = 9.98FDD122 pKa = 3.84SFTYY126 pKa = 10.04SQPTCQYY133 pKa = 9.62YY134 pKa = 9.73WMTSHH139 pKa = 7.77TIGLYY144 pKa = 8.45RR145 pKa = 11.84CEE147 pKa = 4.06RR148 pKa = 11.84ARR150 pKa = 11.84GHH152 pKa = 7.2IIYY155 pKa = 10.1KK156 pKa = 10.34HH157 pKa = 6.09GGLPHH162 pKa = 6.21STLSSNIPCDD172 pKa = 3.73YY173 pKa = 10.63MKK175 pKa = 11.06GEE177 pKa = 4.38CRR179 pKa = 11.84LGLGRR184 pKa = 11.84WMFWEE189 pKa = 4.0VDD191 pKa = 3.42EE192 pKa = 6.11RR193 pKa = 11.84SQDD196 pKa = 3.39DD197 pKa = 3.1WSYY200 pKa = 10.64TYY202 pKa = 11.48NNTAVCDD209 pKa = 4.02GRR211 pKa = 11.84RR212 pKa = 11.84VALTSKK218 pKa = 10.96AIGFGLKK225 pKa = 10.35EE226 pKa = 4.5EE227 pKa = 4.51GCKK230 pKa = 9.3WSGWKK235 pKa = 8.59GTVEE239 pKa = 4.22GVFLKK244 pKa = 10.28WEE246 pKa = 4.28SYY248 pKa = 9.2RR249 pKa = 11.84VRR251 pKa = 11.84SNRR254 pKa = 11.84RR255 pKa = 11.84KK256 pKa = 9.78RR257 pKa = 11.84STDD260 pKa = 2.75RR261 pKa = 11.84RR262 pKa = 11.84FGTLHH267 pKa = 6.61EE268 pKa = 4.67FLASALDD275 pKa = 3.79TMSGTFCEE283 pKa = 4.4TMIGVVPASSLIAHH297 pKa = 6.71SNPSVYY303 pKa = 10.62ARR305 pKa = 11.84LLLEE309 pKa = 4.18NDD311 pKa = 3.33NLVAEE316 pKa = 5.03AYY318 pKa = 10.6GDD320 pKa = 4.58FLIIWQCQAIPIKK333 pKa = 9.89PRR335 pKa = 11.84NGSCYY340 pKa = 7.61STPRR344 pKa = 11.84IEE346 pKa = 4.04YY347 pKa = 9.34WSMGEE352 pKa = 3.85WKK354 pKa = 10.18EE355 pKa = 4.28GYY357 pKa = 10.27LDD359 pKa = 3.2ATMEE363 pKa = 4.04IRR365 pKa = 11.84TDD367 pKa = 3.51SPRR370 pKa = 11.84VSCSHH375 pKa = 6.51HH376 pKa = 5.75SLYY379 pKa = 10.95YY380 pKa = 10.59AINDD384 pKa = 3.43TAYY387 pKa = 9.78IEE389 pKa = 4.59NGEE392 pKa = 4.11LTYY395 pKa = 10.35PLSVTTLSFKK405 pKa = 10.63TKK407 pKa = 10.69DD408 pKa = 3.39LLTAFTPNFTHH419 pKa = 7.21VYY421 pKa = 9.63FSPPPLDD428 pKa = 3.29IEE430 pKa = 4.15LRR432 pKa = 11.84DD433 pKa = 3.68VIGIQDD439 pKa = 3.83RR440 pKa = 11.84LVEE443 pKa = 4.22HH444 pKa = 6.72ARR446 pKa = 11.84HH447 pKa = 6.08LGASIGGTGGGHH459 pKa = 7.07GLTNVPGFKK468 pKa = 10.41FGFLDD473 pKa = 3.46NFVAFLQTVGSMGGLIYY490 pKa = 9.75IICMLLKK497 pKa = 10.64AMRR500 pKa = 11.84PAPRR504 pKa = 11.84AIRR507 pKa = 11.84FVPQGG512 pKa = 3.18

Molecular weight:
57.53 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2683

364

1807

894.3

100.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.634 ± 0.669

2.497 ± 0.476

5.591 ± 0.285

5.479 ± 0.242

3.69 ± 0.253

6.933 ± 0.708

2.572 ± 0.27

5.963 ± 0.248

5.143 ± 0.506

10.287 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.184

3.317 ± 0.348

5.143 ± 0.194

3.429 ± 0.486

5.442 ± 0.395

8.013 ± 0.596

5.852 ± 0.739

5.703 ± 0.373

1.565 ± 0.351

3.914 ± 0.224

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski