Granulicella sp. GAS466

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Granulicella; unclassified Granulicella

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5117 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1JGW7|A0A3N1JGW7_9BACT CzcA family heavy metal efflux pump OS=Granulicella sp. GAS466 OX=2485170 GN=EDE14_0179 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84LFSLAVLASFLSAGSLAAVADD23 pKa = 5.31AIPYY27 pKa = 10.09GNIGNIAPQYY37 pKa = 10.72NFTATATGNVTGYY50 pKa = 9.93FYY52 pKa = 11.22AFSAGDD58 pKa = 3.41TDD60 pKa = 4.39FVRR63 pKa = 11.84MIDD66 pKa = 3.43TTTNTTSSWNFEE78 pKa = 4.25NQTTAVGTTADD89 pKa = 4.07FGHH92 pKa = 6.46VNAGDD97 pKa = 3.41ILVFEE102 pKa = 5.62LYY104 pKa = 10.25DD105 pKa = 3.45ASGNFTYY112 pKa = 10.68ASDD115 pKa = 3.77PAYY118 pKa = 10.93SDD120 pKa = 5.0DD121 pKa = 5.13GINHH125 pKa = 7.81AYY127 pKa = 6.1TTSFTAATVNGVDD140 pKa = 5.03FPAGTYY146 pKa = 10.07IGMEE150 pKa = 4.99DD151 pKa = 3.31IFQGGDD157 pKa = 2.74MDD159 pKa = 4.84YY160 pKa = 11.49NDD162 pKa = 4.36DD163 pKa = 3.69QFVFTNVTTTPAPEE177 pKa = 4.77PGSLLLLGTGAFGVVGILRR196 pKa = 11.84RR197 pKa = 11.84KK198 pKa = 8.78MRR200 pKa = 11.84LNN202 pKa = 3.37

Molecular weight: 21.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1JN30|A0A3N1JN30_9BACT Regulator of protease activity HflC (Stomatin/prohibitin superfamily) OS=Granulicella sp. GAS466 OX=2485170 GN=EDE14_1756 PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.69RR13 pKa = 11.84AKK15 pKa = 9.55THH17 pKa = 5.53GFLSRR22 pKa = 11.84MKK24 pKa = 8.98TKK26 pKa = 10.72AGAAVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.72KK42 pKa = 10.29IAVSAGFRR50 pKa = 11.84DD51 pKa = 3.66

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5117

0

5117

1782126

29

9250

348.3

38.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.599 ± 0.052

0.891 ± 0.012

5.034 ± 0.028

5.21 ± 0.045

3.946 ± 0.026

7.979 ± 0.04

2.264 ± 0.019

5.223 ± 0.03

3.77 ± 0.037

9.862 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.227 ± 0.021

3.56 ± 0.037

5.439 ± 0.024

3.793 ± 0.025

5.964 ± 0.046

6.379 ± 0.033

6.39 ± 0.076

7.28 ± 0.035

1.393 ± 0.013

2.797 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski