Equus caballus (Horse)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria;

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44491 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q2LMH3|A0A3Q2LMH3_HORSE Prostaglandin F synthase 1-like OS=Equus caballus OX=9796 GN=LOC111771772 PE=3 SV=1
II1 pKa = 6.74GTSQNVSTKK10 pKa = 11.02DD11 pKa = 3.34PVTSPEE17 pKa = 3.83NSEE20 pKa = 3.88TRR22 pKa = 11.84EE23 pKa = 4.01NYY25 pKa = 7.56PTDD28 pKa = 3.82DD29 pKa = 3.59VEE31 pKa = 4.43TTEE34 pKa = 4.06DD35 pKa = 4.04HH36 pKa = 6.96IIEE39 pKa = 4.23ALGTNEE45 pKa = 5.68DD46 pKa = 4.49YY47 pKa = 8.18TTEE50 pKa = 3.99PLEE53 pKa = 4.05TLEE56 pKa = 5.84DD57 pKa = 3.98YY58 pKa = 7.59TTADD62 pKa = 4.33LGPTEE67 pKa = 6.0DD68 pKa = 5.59NITDD72 pKa = 3.77DD73 pKa = 4.13LGTIEE78 pKa = 4.67DD79 pKa = 4.51HH80 pKa = 7.16VIEE83 pKa = 4.86APGTTEE89 pKa = 5.69DD90 pKa = 4.06YY91 pKa = 8.14TTEE94 pKa = 3.85HH95 pKa = 7.13LEE97 pKa = 3.78TSEE100 pKa = 4.76YY101 pKa = 9.88YY102 pKa = 7.59TTTDD106 pKa = 2.35SGAYY110 pKa = 9.85DD111 pKa = 4.62DD112 pKa = 4.53YY113 pKa = 10.32TTVALGPEE121 pKa = 4.77DD122 pKa = 4.15YY123 pKa = 7.71TTADD127 pKa = 3.56LGTTKK132 pKa = 10.87DD133 pKa = 3.49SFAADD138 pKa = 3.63LGTIEE143 pKa = 6.17DD144 pKa = 5.36YY145 pKa = 10.28ITDD148 pKa = 3.94DD149 pKa = 3.34PRR151 pKa = 11.84TTEE154 pKa = 4.89DD155 pKa = 3.81YY156 pKa = 7.79TTVDD160 pKa = 3.44LGTTEE165 pKa = 5.56DD166 pKa = 4.93SFTADD171 pKa = 3.05PGTTKK176 pKa = 10.81DD177 pKa = 3.54YY178 pKa = 7.7TTADD182 pKa = 3.57LGPTEE187 pKa = 4.55DD188 pKa = 4.55HH189 pKa = 6.87VIHH192 pKa = 6.77GPGTIEE198 pKa = 5.74DD199 pKa = 4.41YY200 pKa = 8.56TTEE203 pKa = 3.93HH204 pKa = 7.05LEE206 pKa = 4.15TPEE209 pKa = 5.91DD210 pKa = 4.29YY211 pKa = 8.69DD212 pKa = 3.9TSDD215 pKa = 3.48LGTTEE220 pKa = 5.1HH221 pKa = 6.32YY222 pKa = 7.56TTVDD226 pKa = 2.85LGTTKK231 pKa = 10.45DD232 pKa = 4.07YY233 pKa = 8.54PTDD236 pKa = 3.8DD237 pKa = 3.79PGITKK242 pKa = 10.17DD243 pKa = 4.27YY244 pKa = 9.91ITVDD248 pKa = 3.14PGTTEE253 pKa = 4.74DD254 pKa = 5.42SFTADD259 pKa = 3.24LGTTEE264 pKa = 5.89DD265 pKa = 3.9YY266 pKa = 10.42TISDD270 pKa = 4.14LGIPADD276 pKa = 3.97SLIVDD281 pKa = 4.79LGTTKK286 pKa = 10.85YY287 pKa = 9.11YY288 pKa = 8.22TTDD291 pKa = 3.21DD292 pKa = 3.75PGITKK297 pKa = 9.91EE298 pKa = 4.25YY299 pKa = 9.05ITVEE303 pKa = 4.11PGTTEE308 pKa = 4.58DD309 pKa = 5.29SFTADD314 pKa = 3.24LGTTEE319 pKa = 6.1DD320 pKa = 4.33YY321 pKa = 7.67TTSDD325 pKa = 3.84LGTTTDD331 pKa = 3.31SFIVDD336 pKa = 4.19LVTTKK341 pKa = 10.76DD342 pKa = 3.6YY343 pKa = 8.03TTDD346 pKa = 3.42DD347 pKa = 3.67PGITKK352 pKa = 10.1DD353 pKa = 3.89YY354 pKa = 10.84ISVEE358 pKa = 3.95PGTTKK363 pKa = 10.97DD364 pKa = 3.52SFTADD369 pKa = 3.23LEE371 pKa = 4.53TTEE374 pKa = 4.76DD375 pKa = 3.06HH376 pKa = 6.79TIADD380 pKa = 4.05RR381 pKa = 11.84GTTKK385 pKa = 10.92DD386 pKa = 4.12SFTTDD391 pKa = 2.6PGTTVDD397 pKa = 5.57SFTADD402 pKa = 2.95LGTTEE407 pKa = 5.88DD408 pKa = 4.21YY409 pKa = 7.68TTADD413 pKa = 3.65LRR415 pKa = 11.84ITTDD419 pKa = 2.76SFTADD424 pKa = 2.98LRR426 pKa = 11.84TTKK429 pKa = 10.51GYY431 pKa = 7.08TTDD434 pKa = 3.97DD435 pKa = 3.67PEE437 pKa = 4.02ITKK440 pKa = 10.59GYY442 pKa = 8.18ITVDD446 pKa = 3.0PGTTEE451 pKa = 4.74DD452 pKa = 5.42SFTADD457 pKa = 3.21LGTTKK462 pKa = 10.93DD463 pKa = 3.25HH464 pKa = 5.83TTADD468 pKa = 3.65LRR470 pKa = 11.84TTVYY474 pKa = 10.85SCTADD479 pKa = 3.24LGTTKK484 pKa = 10.55DD485 pKa = 3.65YY486 pKa = 7.7TTADD490 pKa = 3.49PGTTEE495 pKa = 5.71DD496 pKa = 4.26YY497 pKa = 7.59TTKK500 pKa = 10.55HH501 pKa = 6.26LNTHH505 pKa = 5.61SEE507 pKa = 4.26GTDD510 pKa = 2.84SVTAVKK516 pKa = 8.77PTGLLTPIGIILISLAATTAIVALFVGLGLIVVSIWPRR554 pKa = 11.84NVKK557 pKa = 10.17EE558 pKa = 3.79IMGMKK563 pKa = 10.17DD564 pKa = 3.12SEE566 pKa = 4.45EE567 pKa = 4.22EE568 pKa = 3.91EE569 pKa = 4.86GMGSS573 pKa = 3.24

Molecular weight: 61.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5F5PPA3|A0A5F5PPA3_HORSE Immunity related GTPase Q OS=Equus caballus OX=9796 GN=IRGQ PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20865

23626

44491

29288982

11

34311

658.3

73.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.05 ± 0.011

2.179 ± 0.01

4.804 ± 0.008

7.183 ± 0.017

3.565 ± 0.007

6.511 ± 0.014

2.542 ± 0.005

4.406 ± 0.011

5.834 ± 0.016

9.93 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.114 ± 0.005

3.62 ± 0.008

6.313 ± 0.017

4.761 ± 0.013

5.763 ± 0.009

8.403 ± 0.015

5.276 ± 0.01

5.96 ± 0.013

1.206 ± 0.004

2.576 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski