Caenorhabditis remanei (Caenorhabditis vulgaris)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3NCH3|E3NCH3_CAERE Uncharacterized protein OS=Caenorhabditis remanei OX=31234 GN=CRE_18208 PE=4 SV=1
MM1 pKa = 7.57SDD3 pKa = 3.09EE4 pKa = 4.15EE5 pKa = 4.4EE6 pKa = 4.96YY7 pKa = 11.3YY8 pKa = 11.02DD9 pKa = 5.0EE10 pKa = 6.45DD11 pKa = 5.59GGGQDD16 pKa = 4.52DD17 pKa = 5.07QGGDD21 pKa = 3.65DD22 pKa = 4.03YY23 pKa = 11.52GYY25 pKa = 11.04SGDD28 pKa = 3.96DD29 pKa = 3.53FGGGGYY35 pKa = 10.32EE36 pKa = 5.81DD37 pKa = 4.42GNQYY41 pKa = 10.77DD42 pKa = 3.59QNQGYY47 pKa = 8.91DD48 pKa = 3.07QNNQYY53 pKa = 9.78GQGYY57 pKa = 7.95GQDD60 pKa = 3.83YY61 pKa = 10.65GNQGYY66 pKa = 10.49GNQQDD71 pKa = 4.29YY72 pKa = 11.6GNQQYY77 pKa = 9.89GQGGGGYY84 pKa = 9.41DD85 pKa = 4.41QEE87 pKa = 4.46YY88 pKa = 9.22QQNDD92 pKa = 3.85YY93 pKa = 11.38QGGGYY98 pKa = 9.88DD99 pKa = 3.53DD100 pKa = 3.84QEE102 pKa = 4.53QYY104 pKa = 11.29EE105 pKa = 4.5EE106 pKa = 4.84DD107 pKa = 3.66PEE109 pKa = 4.25EE110 pKa = 4.64PEE112 pKa = 4.3EE113 pKa = 4.39EE114 pKa = 4.14EE115 pKa = 4.79DD116 pKa = 5.56PEE118 pKa = 5.79PEE120 pKa = 4.01DD121 pKa = 4.0QEE123 pKa = 4.27VDD125 pKa = 3.33EE126 pKa = 4.95SEE128 pKa = 4.2EE129 pKa = 4.21DD130 pKa = 3.75VVEE133 pKa = 4.05TSEE136 pKa = 4.52DD137 pKa = 3.53VEE139 pKa = 4.35EE140 pKa = 4.82EE141 pKa = 4.16EE142 pKa = 5.48EE143 pKa = 4.15EE144 pKa = 4.36DD145 pKa = 4.75DD146 pKa = 6.3GYY148 pKa = 9.24TQVAAHH154 pKa = 6.62TNQASDD160 pKa = 3.67GNGGDD165 pKa = 4.64GNNADD170 pKa = 4.54HH171 pKa = 7.19GLGSLFKK178 pKa = 10.85GAMDD182 pKa = 4.28GFGGGGFDD190 pKa = 4.38GVVGSIGSLISQGGGDD206 pKa = 3.7STGGLTNVFSSGGMEE221 pKa = 4.23SIVGNLISSASHH233 pKa = 5.09QFFGINPSTGAIIGAIAGNIIFQMGGQNNSLSSIGKK269 pKa = 9.12VVLDD273 pKa = 4.54NIISGKK279 pKa = 8.45FKK281 pKa = 10.81RR282 pKa = 11.84DD283 pKa = 3.2IQPFTPGGTVPGLGFQQQFQTINFQQEE310 pKa = 3.75RR311 pKa = 11.84QRR313 pKa = 11.84CLEE316 pKa = 3.71QRR318 pKa = 11.84ILFEE322 pKa = 5.48DD323 pKa = 3.86PQFPANDD330 pKa = 3.04SSLYY334 pKa = 10.21YY335 pKa = 9.17KK336 pKa = 9.69TRR338 pKa = 11.84PDD340 pKa = 3.65EE341 pKa = 4.7PIVWKK346 pKa = 10.5RR347 pKa = 11.84PGEE350 pKa = 4.06IYY352 pKa = 10.41EE353 pKa = 4.1NPQLIVGEE361 pKa = 4.23KK362 pKa = 10.33SRR364 pKa = 11.84FDD366 pKa = 3.83VKK368 pKa = 10.73QGALGDD374 pKa = 4.03CWLLAAVANLTLRR387 pKa = 11.84DD388 pKa = 3.59EE389 pKa = 4.3LFYY392 pKa = 11.02RR393 pKa = 11.84VVPPDD398 pKa = 3.15QSFTEE403 pKa = 4.28NYY405 pKa = 10.35AGIFHH410 pKa = 6.35FQFWRR415 pKa = 11.84YY416 pKa = 7.49GQWVDD421 pKa = 3.97VVIDD425 pKa = 3.94DD426 pKa = 5.2RR427 pKa = 11.84LPTVDD432 pKa = 5.42GRR434 pKa = 11.84LCYY437 pKa = 9.71MRR439 pKa = 11.84SQEE442 pKa = 4.75NNEE445 pKa = 3.83FWSALLEE452 pKa = 3.89KK453 pKa = 10.25AYY455 pKa = 10.72AKK457 pKa = 10.62LYY459 pKa = 10.57GGYY462 pKa = 9.4EE463 pKa = 4.08HH464 pKa = 7.66LDD466 pKa = 3.47GGTTAEE472 pKa = 4.25ALEE475 pKa = 4.92DD476 pKa = 3.74FTGGLTEE483 pKa = 5.66FFDD486 pKa = 4.88LEE488 pKa = 4.58KK489 pKa = 10.84GDD491 pKa = 3.99KK492 pKa = 9.35STILAMLVRR501 pKa = 11.84GMQMGSLFGCSIDD514 pKa = 3.61ADD516 pKa = 4.18EE517 pKa = 4.67NVKK520 pKa = 9.91EE521 pKa = 4.07AQLTNGLVRR530 pKa = 11.84GHH532 pKa = 7.28AYY534 pKa = 10.55SITAIQTVNTYY545 pKa = 10.57SPSPLNQWNPTPPISLQFSS564 pKa = 3.69

Molecular weight: 61.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3LMT0|E3LMT0_CAERE Uncharacterized protein OS=Caenorhabditis remanei OX=31234 GN=CRE_28500 PE=4 SV=1
MM1 pKa = 7.28NGGRR5 pKa = 11.84KK6 pKa = 8.85RR7 pKa = 11.84RR8 pKa = 11.84FPVTVAACSILVLVISVVTAQQVFDD33 pKa = 4.65GNRR36 pKa = 11.84QPAFVRR42 pKa = 11.84RR43 pKa = 11.84QPAFQQQQPNQIQFQQNRR61 pKa = 11.84NFVLPSASPLRR72 pKa = 11.84HH73 pKa = 6.05RR74 pKa = 11.84NLFTRR79 pKa = 11.84QISHH83 pKa = 5.84RR84 pKa = 11.84HH85 pKa = 4.81RR86 pKa = 11.84PRR88 pKa = 11.84HH89 pKa = 5.42RR90 pKa = 11.84SSLRR94 pKa = 11.84KK95 pKa = 7.03TTHH98 pKa = 5.77QPQQAHH104 pKa = 4.73LTSRR108 pKa = 11.84RR109 pKa = 11.84VIPTLSHH116 pKa = 5.82KK117 pKa = 9.08RR118 pKa = 11.84TRR120 pKa = 11.84SRR122 pKa = 11.84TVVSSLSSRR131 pKa = 11.84TKK133 pKa = 9.63PNVTRR138 pKa = 11.84VVNANQRR145 pKa = 11.84TTTIKK150 pKa = 10.59SNNSRR155 pKa = 11.84RR156 pKa = 11.84QQQQFQPRR164 pKa = 11.84QPFMTQRR171 pKa = 11.84QQQFRR176 pKa = 11.84FQPQPQRR183 pKa = 11.84QQIFQRR189 pKa = 11.84VQRR192 pKa = 11.84VQNFQRR198 pKa = 11.84APIQNQQFFVRR209 pKa = 11.84QALNN213 pKa = 3.06

Molecular weight: 25.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31243

9

31252

12547433

9

13921

401.5

45.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.845 ± 0.015

1.973 ± 0.01

5.308 ± 0.012

6.916 ± 0.023

4.776 ± 0.016

5.127 ± 0.016

2.298 ± 0.007

6.225 ± 0.014

6.615 ± 0.018

8.661 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.009

4.939 ± 0.012

4.889 ± 0.021

4.012 ± 0.014

5.337 ± 0.015

7.961 ± 0.021

5.893 ± 0.019

6.242 ± 0.011

1.126 ± 0.004

3.211 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski