Bacteroidales bacterium Barb7

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A180FT70|A0A180FT70_9BACT Xylose isomerase OS=Bacteroidales bacterium Barb7 OX=1633203 GN=xylA PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 10.63NEE4 pKa = 3.98AQHH7 pKa = 6.82LIDD10 pKa = 4.57RR11 pKa = 11.84FLSARR16 pKa = 11.84NEE18 pKa = 3.66GRR20 pKa = 11.84EE21 pKa = 4.05AYY23 pKa = 10.27FDD25 pKa = 3.95ADD27 pKa = 4.41EE28 pKa = 5.68IDD30 pKa = 5.41DD31 pKa = 4.36LLEE34 pKa = 4.34GCEE37 pKa = 4.16DD38 pKa = 4.24KK39 pKa = 11.37EE40 pKa = 5.27DD41 pKa = 3.82FDD43 pKa = 5.86LYY45 pKa = 11.38EE46 pKa = 4.26EE47 pKa = 4.32VLALGLKK54 pKa = 9.13LHH56 pKa = 7.11PEE58 pKa = 4.33NIGLQTKK65 pKa = 9.86RR66 pKa = 11.84CWQYY70 pKa = 11.12VVEE73 pKa = 4.48EE74 pKa = 4.47NYY76 pKa = 7.72TTALEE81 pKa = 4.59LIEE84 pKa = 4.7QIGATGDD91 pKa = 3.54QEE93 pKa = 4.21IEE95 pKa = 4.05AVQLEE100 pKa = 5.14CYY102 pKa = 10.62LMLDD106 pKa = 3.52MTDD109 pKa = 3.25EE110 pKa = 4.56AEE112 pKa = 4.27ALSEE116 pKa = 4.18KK117 pKa = 9.97MIAEE121 pKa = 4.3GRR123 pKa = 11.84EE124 pKa = 4.03SMDD127 pKa = 3.76LVFEE131 pKa = 4.61YY132 pKa = 10.18IVPLLNDD139 pKa = 3.11KK140 pKa = 10.7EE141 pKa = 4.44MYY143 pKa = 10.43SLALEE148 pKa = 4.23YY149 pKa = 11.05VNRR152 pKa = 11.84GLEE155 pKa = 4.33LYY157 pKa = 9.93PDD159 pKa = 3.69SLVLKK164 pKa = 10.56EE165 pKa = 4.13EE166 pKa = 4.38LCFLMEE172 pKa = 3.99VAEE175 pKa = 4.55DD176 pKa = 3.79LEE178 pKa = 4.72GAIDD182 pKa = 4.26LCNAMIDD189 pKa = 3.83EE190 pKa = 5.04DD191 pKa = 4.66PYY193 pKa = 11.43SYY195 pKa = 10.78EE196 pKa = 3.66YY197 pKa = 10.55WFTLGRR203 pKa = 11.84LYY205 pKa = 11.1SLTEE209 pKa = 4.01EE210 pKa = 4.18FEE212 pKa = 4.21KK213 pKa = 11.1AIDD216 pKa = 3.9AFDD219 pKa = 4.04FAQTCEE225 pKa = 4.32EE226 pKa = 4.56PDD228 pKa = 4.07SEE230 pKa = 5.08LKK232 pKa = 10.63LLKK235 pKa = 10.16AYY237 pKa = 10.56CFFMSDD243 pKa = 3.44KK244 pKa = 11.2YY245 pKa = 11.26EE246 pKa = 4.19EE247 pKa = 4.11ALKK250 pKa = 10.78LYY252 pKa = 10.34TEE254 pKa = 4.28LLKK257 pKa = 11.11DD258 pKa = 3.57EE259 pKa = 4.9EE260 pKa = 4.76KK261 pKa = 11.02NGEE264 pKa = 4.23DD265 pKa = 5.15GEE267 pKa = 5.07GDD269 pKa = 3.71DD270 pKa = 5.24YY271 pKa = 11.39ILEE274 pKa = 4.22RR275 pKa = 11.84VRR277 pKa = 11.84PLLAEE282 pKa = 4.56CYY284 pKa = 8.12MKK286 pKa = 11.06LFDD289 pKa = 5.07YY290 pKa = 10.03EE291 pKa = 3.82AAYY294 pKa = 10.48LILHH298 pKa = 6.42EE299 pKa = 5.64LIADD303 pKa = 3.87DD304 pKa = 5.48AEE306 pKa = 4.31AAGYY310 pKa = 7.57EE311 pKa = 4.3TDD313 pKa = 4.13NADD316 pKa = 3.77TEE318 pKa = 4.86VNGADD323 pKa = 3.87TEE325 pKa = 4.76ADD327 pKa = 3.69DD328 pKa = 4.69TDD330 pKa = 3.98TDD332 pKa = 3.76STDD335 pKa = 3.33AARR338 pKa = 11.84KK339 pKa = 9.87DD340 pKa = 3.69YY341 pKa = 11.22SPYY344 pKa = 9.79IYY346 pKa = 10.9YY347 pKa = 7.49MHH349 pKa = 7.46CCAQTDD355 pKa = 3.95RR356 pKa = 11.84KK357 pKa = 10.22QDD359 pKa = 3.38ALNALEE365 pKa = 4.11TAATVFDD372 pKa = 4.58GSAHH376 pKa = 5.66VLSLMAYY383 pKa = 9.82AYY385 pKa = 9.66MEE387 pKa = 4.27SAEE390 pKa = 4.29NDD392 pKa = 3.58DD393 pKa = 4.75NLDD396 pKa = 3.78VINKK400 pKa = 9.28LFEE403 pKa = 5.87LIDD406 pKa = 4.32DD407 pKa = 5.01PGDD410 pKa = 4.04DD411 pKa = 4.94LEE413 pKa = 5.26EE414 pKa = 4.38EE415 pKa = 4.41ADD417 pKa = 3.71GDD419 pKa = 4.07NPSGKK424 pKa = 9.96DD425 pKa = 3.45GFSEE429 pKa = 4.73TIGKK433 pKa = 8.06MLEE436 pKa = 4.16YY437 pKa = 10.24FKK439 pKa = 10.7KK440 pKa = 10.29IYY442 pKa = 9.9AANPDD447 pKa = 3.25QPYY450 pKa = 10.56INLYY454 pKa = 10.09LATAYY459 pKa = 10.62LINDD463 pKa = 3.48DD464 pKa = 3.65MQNFEE469 pKa = 3.9KK470 pKa = 10.44HH471 pKa = 5.65YY472 pKa = 9.78NLLSAEE478 pKa = 4.34EE479 pKa = 4.08LDD481 pKa = 5.01SYY483 pKa = 10.26AKK485 pKa = 10.26KK486 pKa = 10.91YY487 pKa = 9.94NAGDD491 pKa = 3.44SRR493 pKa = 11.84ALRR496 pKa = 11.84TIAEE500 pKa = 4.12KK501 pKa = 10.66HH502 pKa = 5.34IGLRR506 pKa = 11.84DD507 pKa = 3.35LAEE510 pKa = 4.63EE511 pKa = 4.16YY512 pKa = 10.61LSKK515 pKa = 10.93PEE517 pKa = 3.94NSNN520 pKa = 3.08

Molecular weight:
59.48 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A180FTK7|A0A180FTK7_9BACT Capsular glucan synthase OS=Bacteroidales bacterium Barb7 OX=1633203 GN=glgA PE=4 SV=1
MM1 pKa = 7.26LTGDD5 pKa = 4.07APPYY9 pKa = 10.4GGVRR13 pKa = 11.84LCRR16 pKa = 11.84VAVYY20 pKa = 8.22GQPVLKK26 pKa = 10.36EE27 pKa = 3.82LLLVSEE33 pKa = 4.68NILRR37 pKa = 11.84YY38 pKa = 8.42FTEE41 pKa = 3.77IDD43 pKa = 3.38IEE45 pKa = 4.42VAPLRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84IINEE56 pKa = 3.89RR57 pKa = 11.84VKK59 pKa = 10.8HH60 pKa = 5.67PKK62 pKa = 10.15LDD64 pKa = 3.34ILNVCRR70 pKa = 11.84FKK72 pKa = 11.24VALLDD77 pKa = 4.49FPHH80 pKa = 6.47HH81 pKa = 6.35TPPAFLRR88 pKa = 11.84LGKK91 pKa = 8.93RR92 pKa = 11.84TVGIDD97 pKa = 3.27VVGIEE102 pKa = 4.59VIGPALIRR110 pKa = 11.84IIRR113 pKa = 11.84HH114 pKa = 3.76IQYY117 pKa = 8.65PQRR120 pKa = 11.84GSIAYY125 pKa = 9.43DD126 pKa = 3.33LLLVRR131 pKa = 11.84EE132 pKa = 4.45KK133 pKa = 10.84LLHH136 pKa = 6.62FDD138 pKa = 3.17GTHH141 pKa = 4.47IVVRR145 pKa = 11.84LVVFGDD151 pKa = 3.46MAGNGVGVFARR162 pKa = 11.84KK163 pKa = 8.04YY164 pKa = 7.87TVNAVPSRR172 pKa = 11.84QGAVLAVVNVVGKK185 pKa = 10.52SGFGEE190 pKa = 4.05AGRR193 pKa = 11.84GKK195 pKa = 9.94VVVRR199 pKa = 11.84RR200 pKa = 11.84GRR202 pKa = 11.84DD203 pKa = 3.53LPCVGVLQAGVDD215 pKa = 3.68LRR217 pKa = 11.84AFKK220 pKa = 10.23IIKK223 pKa = 10.17VGILNVAACVLALVMTCNQIGKK245 pKa = 9.12LRR247 pKa = 11.84LKK249 pKa = 10.63GYY251 pKa = 9.35LARR254 pKa = 11.84TGQRR258 pKa = 11.84QQRR261 pKa = 11.84QLVEE265 pKa = 3.77QVGQLLALRR274 pKa = 11.84LVGCVQPPQQIINRR288 pKa = 11.84LIPYY292 pKa = 9.75GSLHH296 pKa = 5.82RR297 pKa = 11.84HH298 pKa = 4.56RR299 pKa = 11.84HH300 pKa = 4.61LQHH303 pKa = 5.3VHH305 pKa = 6.18HH306 pKa = 6.86GAAQRR311 pKa = 11.84EE312 pKa = 4.21ILIEE316 pKa = 4.24LIIHH320 pKa = 5.83IHH322 pKa = 5.61AKK324 pKa = 9.9HH325 pKa = 6.1RR326 pKa = 11.84LALHH330 pKa = 5.92TEE332 pKa = 3.95HH333 pKa = 6.98GLVFQRR339 pKa = 11.84NTDD342 pKa = 3.24RR343 pKa = 11.84RR344 pKa = 11.84IACHH348 pKa = 5.77NALVEE353 pKa = 4.36DD354 pKa = 4.74CNRR357 pKa = 11.84PHH359 pKa = 7.62DD360 pKa = 4.57IIHH363 pKa = 6.74RR364 pKa = 11.84IIRR367 pKa = 11.84ILGKK371 pKa = 10.07LCPAGCHH378 pKa = 6.24PYY380 pKa = 10.42RR381 pKa = 11.84PASHH385 pKa = 6.68IRR387 pKa = 11.84CPQLNLRR394 pKa = 11.84AA395 pKa = 4.03

Molecular weight:
44.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2857

0

2857

695753

29

2053

243.5

27.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.637 ± 0.066

1.324 ± 0.025

5.301 ± 0.036

6.184 ± 0.05

4.943 ± 0.039

7.098 ± 0.06

2.067 ± 0.025

6.875 ± 0.057

6.211 ± 0.057

9.225 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.027

4.807 ± 0.047

3.844 ± 0.036

3.323 ± 0.028

5.131 ± 0.043

6.323 ± 0.047

5.645 ± 0.043

6.774 ± 0.056

0.977 ± 0.019

4.021 ± 0.043

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski