Flavobacteriaceae bacterium

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3010 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9UP63|A0A3A9UP63_9FLAO Phosphate transport system permease protein OS=Flavobacteriaceae bacterium OX=1871037 GN=pstC PE=3 SV=1
MM1 pKa = 7.31TKK3 pKa = 9.94IIVLFLLVLALKK15 pKa = 8.7ITPSHH20 pKa = 5.97SQTTLTAGDD29 pKa = 3.73IAITGYY35 pKa = 8.31NTDD38 pKa = 4.07GDD40 pKa = 4.11DD41 pKa = 3.3QVAFVLLTDD50 pKa = 3.61ITVGTEE56 pKa = 3.16IRR58 pKa = 11.84FTDD61 pKa = 4.86RR62 pKa = 11.84GWLDD66 pKa = 3.31TNAFRR71 pKa = 11.84IGNTGRR77 pKa = 11.84EE78 pKa = 4.24GTLIWVADD86 pKa = 3.84TDD88 pKa = 4.66LSCGSQIILTSANDD102 pKa = 3.78GTLTISPNIGSLTEE116 pKa = 3.74VDD118 pKa = 3.66DD119 pKa = 4.75FEE121 pKa = 6.1IRR123 pKa = 11.84GQGDD127 pKa = 3.3QILAYY132 pKa = 10.19QGTDD136 pKa = 3.23DD137 pKa = 4.86SPTFIYY143 pKa = 10.68ALNFNNPGWSTTAGNQQEE161 pKa = 4.46SALPIGLTDD170 pKa = 3.98GVNSVDD176 pKa = 3.57ISGDD180 pKa = 3.17IDD182 pKa = 3.54NGTYY186 pKa = 10.38NCAVTTLPDD195 pKa = 4.71AILASVSDD203 pKa = 3.66AANWNTSDD211 pKa = 4.01GDD213 pKa = 4.19GNQSLTLGQCLFSCTSIIQTVLTAGDD239 pKa = 3.78IVITGYY245 pKa = 8.26NTDD248 pKa = 3.98GNDD251 pKa = 2.89QVAFVLLTDD260 pKa = 3.83ITAGTEE266 pKa = 3.43IRR268 pKa = 11.84FTDD271 pKa = 4.45RR272 pKa = 11.84GWLDD276 pKa = 2.98TDD278 pKa = 3.79AFRR281 pKa = 11.84VGNTDD286 pKa = 4.33RR287 pKa = 11.84EE288 pKa = 4.59GTLIWTANTDD298 pKa = 4.22LSCGTQIILTSANNGTLTISPNTGILTEE326 pKa = 4.38EE327 pKa = 4.62DD328 pKa = 3.38DD329 pKa = 4.8FEE331 pKa = 5.37IRR333 pKa = 11.84GQGDD337 pKa = 3.3QILAYY342 pKa = 10.19QGTDD346 pKa = 3.23DD347 pKa = 4.86SPTFIYY353 pKa = 10.68ALNFNNPGWSVTAGNQQEE371 pKa = 4.41SALPIGLADD380 pKa = 3.87GVNSVDD386 pKa = 3.76ISGDD390 pKa = 3.25IDD392 pKa = 3.51NGAYY396 pKa = 10.23DD397 pKa = 4.08CAVTTSPEE405 pKa = 4.51LILTAVSDD413 pKa = 3.61ATNWDD418 pKa = 3.64TSDD421 pKa = 3.34GGGNQSLTLGLCTFDD436 pKa = 5.08CSVICPTTTTWNGTTWDD453 pKa = 3.59NGIPNTTVAAIINGAYY469 pKa = 5.58TTGVNGNISACSLAVNSGFRR489 pKa = 11.84LSISNSTFIEE499 pKa = 4.03IEE501 pKa = 3.75SDD503 pKa = 3.76VVINGEE509 pKa = 4.2IIVEE513 pKa = 4.08SSGNFVQNIDD523 pKa = 3.25SSTYY527 pKa = 8.08TNNGAMSRR535 pKa = 11.84VNKK538 pKa = 7.25VTPVKK543 pKa = 10.46QDD545 pKa = 2.81WFFFTYY551 pKa = 9.48WSSPVSGLTVDD562 pKa = 5.79DD563 pKa = 4.49VFATNPANRR572 pKa = 11.84RR573 pKa = 11.84FIFNANNYY581 pKa = 10.09LDD583 pKa = 4.7LNEE586 pKa = 6.06DD587 pKa = 4.3GFDD590 pKa = 4.09DD591 pKa = 5.09DD592 pKa = 4.92ANAYY596 pKa = 9.16EE597 pKa = 4.79LVSGSDD603 pKa = 3.44PLIPGVGYY611 pKa = 10.55AITEE615 pKa = 4.01NQQFFIPGSTAQATFDD631 pKa = 3.51GTFNNGLIEE640 pKa = 4.14VPIAYY645 pKa = 9.98DD646 pKa = 3.43SANVAHH652 pKa = 6.62YY653 pKa = 10.88NFIGNPYY660 pKa = 9.09PSAIDD665 pKa = 3.56FEE667 pKa = 4.92IFQATNSSLIGGIAYY682 pKa = 9.41LWSQSTPPSANNPGNQTVNFSQNDD706 pKa = 3.63YY707 pKa = 9.45ATYY710 pKa = 9.75TIGSGGAAGASGIIPTQYY728 pKa = 10.02IPSGQGFFIPSVGAGNAVFKK748 pKa = 11.18NSMRR752 pKa = 11.84VASIDD757 pKa = 3.58SNNQFFGTEE766 pKa = 4.17EE767 pKa = 3.78NSLTLNSNPTVNSNDD782 pKa = 3.6LLIDD786 pKa = 3.74NEE788 pKa = 4.09NKK790 pKa = 9.76IWINLKK796 pKa = 10.1SDD798 pKa = 3.16NGIFNQILVAYY809 pKa = 9.98VGGATDD815 pKa = 4.12AYY817 pKa = 11.03DD818 pKa = 3.84GFSYY822 pKa = 10.47DD823 pKa = 3.61APRR826 pKa = 11.84VLPIGTSAILYY837 pKa = 7.44TFIEE841 pKa = 4.82DD842 pKa = 4.87DD843 pKa = 3.52EE844 pKa = 6.89DD845 pKa = 4.91DD846 pKa = 3.68IKK848 pKa = 11.23FVIQGKK854 pKa = 9.89DD855 pKa = 2.89INSINEE861 pKa = 3.63NEE863 pKa = 4.28IIHH866 pKa = 6.7LGFEE870 pKa = 4.52TNIEE874 pKa = 4.19VPTLYY879 pKa = 10.17TLSLDD884 pKa = 3.56QFEE887 pKa = 4.91GAFIEE892 pKa = 4.38NSTIFLKK899 pKa = 11.09DD900 pKa = 3.2NLLDD904 pKa = 3.66VMHH907 pKa = 6.8NLSEE911 pKa = 4.15GDD913 pKa = 3.68YY914 pKa = 10.51EE915 pKa = 4.39FTSEE919 pKa = 3.94VGTFEE924 pKa = 5.22EE925 pKa = 4.67RR926 pKa = 11.84FQIQFVSEE934 pKa = 4.08TLSIDD939 pKa = 3.44EE940 pKa = 4.4NLVIEE945 pKa = 4.36NEE947 pKa = 4.11LVIIEE952 pKa = 4.72LNNNDD957 pKa = 3.14VQFKK961 pKa = 10.71VSGNLEE967 pKa = 3.94MEE969 pKa = 4.48SIKK972 pKa = 10.7IIDD975 pKa = 3.65LNGRR979 pKa = 11.84VLYY982 pKa = 10.36NFKK985 pKa = 10.95AQGSDD990 pKa = 2.77NTYY993 pKa = 11.27NLSKK997 pKa = 10.68LNNSVYY1003 pKa = 10.12IAQIRR1008 pKa = 11.84LTNGVLISKK1017 pKa = 10.2KK1018 pKa = 10.0ALKK1021 pKa = 10.59RR1022 pKa = 11.84NN1023 pKa = 3.56

Molecular weight: 110.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9UMP3|A0A3A9UMP3_9FLAO RDD family protein OS=Flavobacteriaceae bacterium OX=1871037 GN=D1817_14665 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.92GRR40 pKa = 11.84KK41 pKa = 8.34KK42 pKa = 10.22ISVSSEE48 pKa = 3.61LRR50 pKa = 11.84HH51 pKa = 6.16KK52 pKa = 10.53KK53 pKa = 10.04

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3010

0

3010

1019847

29

3644

338.8

38.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.87 ± 0.043

0.69 ± 0.015

5.505 ± 0.044

6.423 ± 0.048

5.348 ± 0.044

6.183 ± 0.051

1.715 ± 0.023

8.892 ± 0.049

7.943 ± 0.079

9.368 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.024

6.775 ± 0.057

3.245 ± 0.025

3.308 ± 0.026

3.447 ± 0.028

6.6 ± 0.039

5.905 ± 0.064

5.82 ± 0.033

1.001 ± 0.018

4.018 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski