Alteromonas sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3562 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N5Y2E1|A0A3N5Y2E1_9ALTE tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase OS=Alteromonas sediminis OX=2259342 GN=miaB PE=3 SV=1
MM1 pKa = 7.18KK2 pKa = 9.56QAKK5 pKa = 9.91VIGGVLATAAFLTSLSSSAEE25 pKa = 3.9DD26 pKa = 3.29FDD28 pKa = 4.91WYY30 pKa = 11.01LKK32 pKa = 9.27PTFGLSTLSDD42 pKa = 3.25QSGRR46 pKa = 11.84VSDD49 pKa = 4.05VLNQTGAVDD58 pKa = 3.74VNLDD62 pKa = 3.25AGLNAGLGVGYY73 pKa = 10.01FVDD76 pKa = 4.62EE77 pKa = 4.22YY78 pKa = 10.76WAVEE82 pKa = 4.23LYY84 pKa = 9.69WEE86 pKa = 4.21YY87 pKa = 11.09RR88 pKa = 11.84SNDD91 pKa = 3.29SEE93 pKa = 4.69TILPGDD99 pKa = 3.67IVFTDD104 pKa = 4.01GNFASSMFALNTSYY118 pKa = 11.29YY119 pKa = 11.06SNLNADD125 pKa = 3.56WRR127 pKa = 11.84WFIGAGIAVLQEE139 pKa = 3.51IDD141 pKa = 4.17IDD143 pKa = 4.36LEE145 pKa = 4.45DD146 pKa = 4.88DD147 pKa = 3.37SGEE150 pKa = 4.08RR151 pKa = 11.84SFSGSGDD158 pKa = 3.18LGFQIFAGIDD168 pKa = 3.39YY169 pKa = 10.18EE170 pKa = 4.62LTQNWSLQAEE180 pKa = 4.46VRR182 pKa = 11.84YY183 pKa = 8.66LTVSGIDD190 pKa = 3.22MVAEE194 pKa = 4.24EE195 pKa = 4.67NVTAGVFQDD204 pKa = 4.09FDD206 pKa = 3.82YY207 pKa = 11.44TPISLQANLTYY218 pKa = 10.92KK219 pKa = 10.63FF220 pKa = 3.82

Molecular weight: 24.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N5Z7U6|A0A3N5Z7U6_9ALTE Chemotaxis signal transduction protein CheV OS=Alteromonas sediminis OX=2259342 GN=DRW07_16645 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3562

0

3562

1208032

28

2034

339.1

37.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.937 ± 0.041

1.005 ± 0.014

5.72 ± 0.036

6.103 ± 0.041

4.271 ± 0.027

6.634 ± 0.042

2.329 ± 0.021

6.16 ± 0.03

5.055 ± 0.036

10.264 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.017

4.276 ± 0.026

3.947 ± 0.026

4.514 ± 0.031

4.675 ± 0.029

6.706 ± 0.034

5.533 ± 0.026

7.084 ± 0.033

1.239 ± 0.016

3.037 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski