Actinorugispora endophytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Actinorugispora

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4593 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6UL49|A0A4R6UL49_9ACTN Putative repeat protein (TIGR03847 family) OS=Actinorugispora endophytica OX=1605990 GN=EV190_12812 PE=4 SV=1
MM1 pKa = 7.34GFVALGAAATLLLSACGGGDD21 pKa = 3.6GGDD24 pKa = 3.57GDD26 pKa = 4.54GGSGAGGVFDD36 pKa = 3.73QASTEE41 pKa = 4.2VVNASDD47 pKa = 3.49ATGGTLRR54 pKa = 11.84YY55 pKa = 9.7AISANMEE62 pKa = 3.94STDD65 pKa = 4.47PGNTYY70 pKa = 10.58YY71 pKa = 11.08GYY73 pKa = 9.13VWNFSRR79 pKa = 11.84YY80 pKa = 6.71YY81 pKa = 11.04ARR83 pKa = 11.84TLLTFSPVPGQASSEE98 pKa = 4.08LLPDD102 pKa = 4.03LAEE105 pKa = 4.86DD106 pKa = 3.79MPEE109 pKa = 3.93VSDD112 pKa = 6.33DD113 pKa = 3.63GTEE116 pKa = 3.76WTIKK120 pKa = 10.28LKK122 pKa = 10.77QGLKK126 pKa = 10.39YY127 pKa = 10.34EE128 pKa = 4.75DD129 pKa = 3.73GSEE132 pKa = 4.19IVAEE136 pKa = 4.51DD137 pKa = 3.01IKK139 pKa = 11.38YY140 pKa = 10.72AIARR144 pKa = 11.84SNFGPQALPNGPKK157 pKa = 10.22YY158 pKa = 10.24FQQLLDD164 pKa = 3.96ADD166 pKa = 4.9DD167 pKa = 4.42YY168 pKa = 11.04EE169 pKa = 5.36GPYY172 pKa = 10.76GGGDD176 pKa = 3.52PLAGFDD182 pKa = 5.43AIEE185 pKa = 4.39TPDD188 pKa = 4.63DD189 pKa = 3.48YY190 pKa = 11.97TLVFKK195 pKa = 10.96LKK197 pKa = 10.8APFADD202 pKa = 4.51FPYY205 pKa = 10.37ILSQTQTAPVPAEE218 pKa = 3.99ADD220 pKa = 2.87TGEE223 pKa = 4.17QYY225 pKa = 10.57QGQVVSSGPYY235 pKa = 9.44KK236 pKa = 10.75FEE238 pKa = 3.99GSYY241 pKa = 10.3TPGDD245 pKa = 3.52GLVLVRR251 pKa = 11.84NDD253 pKa = 2.92QWDD256 pKa = 3.93PEE258 pKa = 4.28SDD260 pKa = 3.51PTRR263 pKa = 11.84EE264 pKa = 4.03ALPDD268 pKa = 3.27RR269 pKa = 11.84VTVEE273 pKa = 3.91EE274 pKa = 4.42GVEE277 pKa = 3.9QNEE280 pKa = 3.65IDD282 pKa = 3.49QRR284 pKa = 11.84LVNGEE289 pKa = 4.53LDD291 pKa = 3.29VDD293 pKa = 4.44LAGTGLGPAMKK304 pKa = 10.3GDD306 pKa = 4.25LLADD310 pKa = 3.57EE311 pKa = 4.71EE312 pKa = 4.35ARR314 pKa = 11.84ANIDD318 pKa = 3.46NPVTGAHH325 pKa = 6.65FYY327 pKa = 10.85VAINTKK333 pKa = 9.48VEE335 pKa = 4.01PLDD338 pKa = 5.41DD339 pKa = 3.91VACRR343 pKa = 11.84QAIQYY348 pKa = 8.02AASRR352 pKa = 11.84EE353 pKa = 4.48GIQRR357 pKa = 11.84AYY359 pKa = 10.2GGEE362 pKa = 4.04FGGEE366 pKa = 3.66IATQVLPPSIPGANPEE382 pKa = 4.2LDD384 pKa = 3.77PYY386 pKa = 10.22EE387 pKa = 4.41AASGTGNVEE396 pKa = 3.68MAQEE400 pKa = 3.94KK401 pKa = 10.81LEE403 pKa = 4.23EE404 pKa = 4.6CGEE407 pKa = 4.1EE408 pKa = 5.01DD409 pKa = 4.48GFTVNIGVRR418 pKa = 11.84SDD420 pKa = 3.44RR421 pKa = 11.84PAEE424 pKa = 4.01VAAVEE429 pKa = 4.67SIQEE433 pKa = 4.0SLAAANITAEE443 pKa = 4.27IKK445 pKa = 10.66SFPSDD450 pKa = 3.01TFTNTQAGSPDD461 pKa = 3.4FVADD465 pKa = 3.7NEE467 pKa = 4.68LGLNYY472 pKa = 9.67YY473 pKa = 10.48GWMSDD478 pKa = 2.85WPSGYY483 pKa = 10.84GYY485 pKa = 9.92MSSILDD491 pKa = 3.46GDD493 pKa = 4.46SIKK496 pKa = 10.82EE497 pKa = 3.95AGNSNISEE505 pKa = 4.32LDD507 pKa = 3.48DD508 pKa = 4.21PEE510 pKa = 5.09INQLFDD516 pKa = 4.94DD517 pKa = 4.31VTQVEE522 pKa = 4.63DD523 pKa = 4.38PEE525 pKa = 4.18EE526 pKa = 3.63QAAIYY531 pKa = 9.78SQIDD535 pKa = 3.85EE536 pKa = 4.65LTMEE540 pKa = 4.77SATILPGVFQKK551 pKa = 10.91SVLYY555 pKa = 10.47RR556 pKa = 11.84PEE558 pKa = 4.05NLTNVYY564 pKa = 9.83FNPSYY569 pKa = 11.38HH570 pKa = 6.5MYY572 pKa = 10.8DD573 pKa = 3.56YY574 pKa = 10.29MALGTTNNEE583 pKa = 3.79

Molecular weight: 62.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6UNQ8|A0A4R6UNQ8_9ACTN Cytochrome P450 OS=Actinorugispora endophytica OX=1605990 GN=EV190_11744 PE=3 SV=1
MM1 pKa = 6.35MTGAAATAAPTARR14 pKa = 11.84AVSAAGPPRR23 pKa = 11.84AVSAANPTSPGAATATATSTARR45 pKa = 11.84AAPTGAVGTTVTRR58 pKa = 11.84VVAVPTAVTVTTARR72 pKa = 11.84VAGTATVTRR81 pKa = 11.84AVAVPTAVTVTTARR95 pKa = 11.84VAGTATVTRR104 pKa = 11.84AVAPVVATTGGAHH117 pKa = 6.1VAPATATTVPVAGTATATRR136 pKa = 11.84AVARR140 pKa = 11.84VVATTARR147 pKa = 11.84AAVPLGAVGMTVLVVMIGVARR168 pKa = 11.84VRR170 pKa = 11.84AVRR173 pKa = 11.84SSAATTARR181 pKa = 11.84VAGTATATRR190 pKa = 11.84VAAVPTAVTVTTARR204 pKa = 11.84VAGTATATRR213 pKa = 11.84VVARR217 pKa = 11.84VRR219 pKa = 11.84AVRR222 pKa = 11.84SNVVTTVPVAGTATVTRR239 pKa = 11.84AVARR243 pKa = 11.84VVATTVRR250 pKa = 11.84AAVPLGAVGMTVLVVMIGVARR271 pKa = 11.84VRR273 pKa = 11.84AVRR276 pKa = 11.84SSAATTAPAAVPPVAGPPSARR297 pKa = 11.84AAPSATCRR305 pKa = 11.84PRR307 pKa = 11.84PRR309 pKa = 11.84PGSAPFARR317 pKa = 11.84STTPATTTRR326 pKa = 11.84PTTRR330 pKa = 11.84VTATSTCPAASGCRR344 pKa = 11.84RR345 pKa = 11.84PSPRR349 pKa = 11.84RR350 pKa = 11.84VWPAGAPARR359 pKa = 11.84SS360 pKa = 3.37

Molecular weight: 34.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4593

0

4593

1481852

29

7981

322.6

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.476 ± 0.057

0.715 ± 0.012

6.237 ± 0.038

6.29 ± 0.04

2.767 ± 0.019

9.654 ± 0.037

2.127 ± 0.018

3.042 ± 0.029

1.579 ± 0.024

10.361 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.765 ± 0.017

1.739 ± 0.018

6.195 ± 0.046

2.349 ± 0.025

8.658 ± 0.038

5.091 ± 0.028

5.532 ± 0.028

8.96 ± 0.044

1.465 ± 0.015

1.997 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski