Arthrobacter phage Giantsbane

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordonvirus; unclassified Gordonvirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9JKW1|A0A6B9JKW1_9CAUD Uncharacterized protein OS=Arthrobacter phage Giantsbane OX=2686082 GN=33 PE=4 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.38LTWDD7 pKa = 3.47VVGEE11 pKa = 4.16RR12 pKa = 11.84FYY14 pKa = 11.34EE15 pKa = 4.37AGTDD19 pKa = 3.41RR20 pKa = 11.84GVLYY24 pKa = 10.72LDD26 pKa = 4.11GVGHH30 pKa = 5.79VWDD33 pKa = 4.14GLVAVEE39 pKa = 4.31QSPSGGEE46 pKa = 3.82PEE48 pKa = 4.84TYY50 pKa = 10.31YY51 pKa = 10.89QDD53 pKa = 3.56GLPYY57 pKa = 9.96ISVSSVEE64 pKa = 4.08EE65 pKa = 3.83FKK67 pKa = 11.38GSIEE71 pKa = 4.41AYY73 pKa = 8.28TYY75 pKa = 10.46PNAFAACDD83 pKa = 3.7GSEE86 pKa = 4.44EE87 pKa = 4.21IAQGLYY93 pKa = 9.96IGQQDD98 pKa = 3.22RR99 pKa = 11.84KK100 pKa = 10.3EE101 pKa = 4.12FGLSYY106 pKa = 9.69RR107 pKa = 11.84TLVGNDD113 pKa = 3.22VEE115 pKa = 4.54EE116 pKa = 4.89LEE118 pKa = 4.86HH119 pKa = 6.89GYY121 pKa = 10.16KK122 pKa = 10.4LHH124 pKa = 7.81IIYY127 pKa = 10.22QCMASPTNRR136 pKa = 11.84SYY138 pKa = 10.66STLGDD143 pKa = 3.57STDD146 pKa = 2.95ASTFNWAITTRR157 pKa = 11.84PVKK160 pKa = 10.78FEE162 pKa = 4.53DD163 pKa = 3.69EE164 pKa = 4.61VFGVRR169 pKa = 11.84YY170 pKa = 8.97GAHH173 pKa = 6.09LTLDD177 pKa = 3.52SRR179 pKa = 11.84EE180 pKa = 4.11VYY182 pKa = 8.57PWAMAAVEE190 pKa = 4.03AVLYY194 pKa = 8.57GTDD197 pKa = 3.39EE198 pKa = 4.47AEE200 pKa = 4.29PRR202 pKa = 11.84LPTPAEE208 pKa = 4.1LLTLFIDD215 pKa = 3.75NAILKK220 pKa = 8.61ITDD223 pKa = 3.69NGDD226 pKa = 3.62GTWTADD232 pKa = 3.55APDD235 pKa = 5.03SILTMLTDD243 pKa = 5.56EE244 pKa = 4.72IFQINWTSANMIDD257 pKa = 3.75EE258 pKa = 4.43DD259 pKa = 4.92TYY261 pKa = 11.19TVSTFF266 pKa = 3.03

Molecular weight: 29.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9JD76|A0A6B9JD76_9CAUD HNH endonuclease OS=Arthrobacter phage Giantsbane OX=2686082 GN=2 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.52PTLAAALFGSAISSAVALVALAIIVRR28 pKa = 11.84LRR30 pKa = 11.84MEE32 pKa = 3.81RR33 pKa = 11.84RR34 pKa = 11.84EE35 pKa = 3.98RR36 pKa = 11.84RR37 pKa = 11.84SDD39 pKa = 3.01AGRR42 pKa = 11.84TIKK45 pKa = 10.53DD46 pKa = 3.2KK47 pKa = 10.96HH48 pKa = 6.36LSPMFGRR55 pKa = 11.84PISDD59 pKa = 3.26WQHH62 pKa = 5.04VFTIRR67 pKa = 11.84PVHH70 pKa = 6.05TLDD73 pKa = 3.94QGWVFCRR80 pKa = 11.84YY81 pKa = 5.8VWRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84IYY88 pKa = 10.18KK89 pKa = 9.63HH90 pKa = 5.63QFLPGGGSDD99 pKa = 3.19YY100 pKa = 10.62WFQYY104 pKa = 9.84LVKK107 pKa = 10.34LQYY110 pKa = 11.09VYY112 pKa = 11.12DD113 pKa = 3.77

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

17828

37

1529

189.7

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.728 ± 0.445

0.662 ± 0.116

6.103 ± 0.265

6.49 ± 0.387

3.73 ± 0.189

7.539 ± 0.479

1.924 ± 0.186

6.052 ± 0.235

6.944 ± 0.436

7.898 ± 0.414

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.95 ± 0.259

4.459 ± 0.192

4.061 ± 0.253

3.444 ± 0.137

5.015 ± 0.33

6.063 ± 0.217

6.31 ± 0.231

6.753 ± 0.2

1.447 ± 0.12

3.427 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski