Glaciecola nitratireducens (strain JCM 12485 / KCTC 12276 / FR1064)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Glaciecola; Glaciecola nitratireducens

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4QJT3|G4QJT3_GLANF Transporter small conductance mechanosensitive ion channel (MscS) family protein OS=Glaciecola nitratireducens (strain JCM 12485 / KCTC 12276 / FR1064) OX=1085623 GN=GNIT_0836 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.39KK3 pKa = 10.32SLLGCALLAAAAVAPVQADD22 pKa = 3.92TLLGAYY28 pKa = 9.59VGAQAWNMGVDD39 pKa = 3.71GGFSNNEE46 pKa = 3.86SVTNFSFDD54 pKa = 3.52DD55 pKa = 3.53QTNGSFYY62 pKa = 11.05VALEE66 pKa = 4.21HH67 pKa = 6.46PVPLIPNIKK76 pKa = 9.71INRR79 pKa = 11.84TMLDD83 pKa = 3.52TNGVTTLTSTFEE95 pKa = 4.41FNDD98 pKa = 3.38EE99 pKa = 4.29VYY101 pKa = 10.88SADD104 pKa = 3.69TNLTTDD110 pKa = 3.1IEE112 pKa = 4.56MTGTDD117 pKa = 4.4FILYY121 pKa = 9.85YY122 pKa = 10.53EE123 pKa = 4.63ILDD126 pKa = 3.92NDD128 pKa = 4.33LVSIDD133 pKa = 3.37VGINGKK139 pKa = 10.39YY140 pKa = 9.16IDD142 pKa = 3.86GTIMVSEE149 pKa = 4.22TSGNRR154 pKa = 11.84MVSEE158 pKa = 4.7DD159 pKa = 3.89FSGVIPMGYY168 pKa = 10.56AKK170 pKa = 10.77AEE172 pKa = 3.95VGLPFTGLGLYY183 pKa = 10.71AEE185 pKa = 5.1GSFLALGDD193 pKa = 3.82STVSDD198 pKa = 4.08FQAAITYY205 pKa = 10.08SFVEE209 pKa = 4.33TLALDD214 pKa = 3.48MTLQAGYY221 pKa = 10.79RR222 pKa = 11.84SMKK225 pKa = 10.41VEE227 pKa = 5.45LDD229 pKa = 4.08DD230 pKa = 6.88LDD232 pKa = 5.37DD233 pKa = 5.74LYY235 pKa = 11.44TNLDD239 pKa = 3.61FKK241 pKa = 11.4GVFVGLEE248 pKa = 3.71FDD250 pKa = 4.17FF251 pKa = 5.96

Molecular weight: 27.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4QNM4|G4QNM4_GLANF Putative cation efflux protein OS=Glaciecola nitratireducens (strain JCM 12485 / KCTC 12276 / FR1064) OX=1085623 GN=GNIT_3488 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.2VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.45GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3653

0

3653

1214097

35

2955

332.4

36.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.627 ± 0.038

0.949 ± 0.014

5.735 ± 0.034

6.175 ± 0.035

4.367 ± 0.03

6.478 ± 0.039

2.056 ± 0.02

6.789 ± 0.032

5.715 ± 0.036

10.131 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.019

4.717 ± 0.03

3.707 ± 0.022

4.351 ± 0.034

4.226 ± 0.027

7.066 ± 0.036

5.395 ± 0.027

6.885 ± 0.03

1.121 ± 0.016

2.97 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski