Mycoplasmataceae bacterium RV_VA103A

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; unclassified Mycoplasmataceae

Average proteome isoelectric point is 7.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1718 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H1SE07|A0A0H1SE07_9MOLU Uncharacterized protein OS=Mycoplasmataceae bacterium RV_VA103A OX=1528105 GN=MRERV_3c030 PE=4 SV=1
MM1 pKa = 7.91LKK3 pKa = 10.82GNWGNVKK10 pKa = 10.34CKK12 pKa = 9.51TCGAEE17 pKa = 4.09EE18 pKa = 4.65YY19 pKa = 10.04ISEE22 pKa = 5.17DD23 pKa = 3.09IPGEE27 pKa = 4.09RR28 pKa = 11.84EE29 pKa = 3.99WYY31 pKa = 9.76VLEE34 pKa = 4.54SEE36 pKa = 4.34TGEE39 pKa = 4.14EE40 pKa = 4.0FDD42 pKa = 4.25MCIDD46 pKa = 4.1CFGSYY51 pKa = 9.45IKK53 pKa = 10.97NVDD56 pKa = 3.32EE57 pKa = 4.99EE58 pKa = 5.41GNFSII63 pKa = 5.74

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H1SCR2|A0A0H1SCR2_9MOLU Uncharacterized protein (Fragment) OS=Mycoplasmataceae bacterium RV_VA103A OX=1528105 GN=MRERV_79c011 PE=4 SV=1
MM1 pKa = 7.1FHH3 pKa = 6.93WGWKK7 pKa = 9.57NILGSRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ILVAFRR21 pKa = 11.84LVQKK25 pKa = 9.93AWRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84GRR32 pKa = 11.84RR33 pKa = 11.84WGKK36 pKa = 10.09

Molecular weight: 4.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1718

0

1718

340732

28

1926

198.3

23.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.56 ± 0.062

1.329 ± 0.032

4.551 ± 0.046

8.903 ± 0.097

4.391 ± 0.065

4.791 ± 0.061

1.555 ± 0.03

7.525 ± 0.053

11.235 ± 0.076

9.958 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.433 ± 0.022

6.599 ± 0.058

3.358 ± 0.045

4.965 ± 0.073

4.327 ± 0.05

5.769 ± 0.055

4.718 ± 0.048

4.404 ± 0.043

1.911 ± 0.032

3.716 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski