Microviridae Fen4707_41

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UK32|A0A0G2UK32_9VIRU Replication protein VP4 OS=Microviridae Fen4707_41 OX=1655654 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84KK3 pKa = 8.37PQEE6 pKa = 3.82ILEE9 pKa = 4.35DD10 pKa = 3.73LGVRR14 pKa = 11.84FAWVNQASWVADD26 pKa = 3.75EE27 pKa = 4.66TAEE30 pKa = 4.04QCGKK34 pKa = 10.2FPSLTDD40 pKa = 3.91PDD42 pKa = 4.18SEE44 pKa = 4.9VYY46 pKa = 9.43TVAEE50 pKa = 4.75LIQRR54 pKa = 11.84HH55 pKa = 4.98EE56 pKa = 4.55RR57 pKa = 11.84GLLTNLNIGKK67 pKa = 9.85SYY69 pKa = 10.53AYY71 pKa = 9.73PEE73 pKa = 4.19FARR76 pKa = 11.84LDD78 pKa = 3.76DD79 pKa = 5.0RR80 pKa = 11.84DD81 pKa = 3.94LEE83 pKa = 5.39KK84 pKa = 11.15DD85 pKa = 3.42LALEE89 pKa = 4.31PTDD92 pKa = 4.22ASHH95 pKa = 7.65LADD98 pKa = 4.62QLDD101 pKa = 4.0NQLKK105 pKa = 10.03PMRR108 pKa = 11.84KK109 pKa = 8.52AQAEE113 pKa = 4.26AKK115 pKa = 8.31TKK117 pKa = 10.47KK118 pKa = 10.43DD119 pKa = 3.38EE120 pKa = 4.33EE121 pKa = 4.2DD122 pKa = 3.04RR123 pKa = 11.84KK124 pKa = 10.24AAEE127 pKa = 3.94DD128 pKa = 3.51AVFNARR134 pKa = 11.84LAAEE138 pKa = 4.49GWVKK142 pKa = 10.5PEE144 pKa = 3.99KK145 pKa = 10.88GGG147 pKa = 3.77

Molecular weight: 16.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UMF1|A0A0G2UMF1_9VIRU Uncharacterized protein OS=Microviridae Fen4707_41 OX=1655654 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.17KK3 pKa = 9.82NCFVLVFSSSCSRR16 pKa = 11.84QSFTIPGNSCKK27 pKa = 10.14AVKK30 pKa = 9.76IRR32 pKa = 11.84CSRR35 pKa = 11.84LRR37 pKa = 11.84VRR39 pKa = 11.84AFRR42 pKa = 11.84PHH44 pKa = 6.68PPP46 pKa = 3.11

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1287

46

537

214.5

24.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.091 ± 1.473

1.476 ± 0.621

5.361 ± 0.882

4.274 ± 1.131

5.128 ± 0.899

6.605 ± 0.964

1.709 ± 0.267

4.04 ± 0.481

5.206 ± 1.611

9.091 ± 0.886

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.865 ± 0.299

5.284 ± 0.632

5.439 ± 0.382

5.128 ± 0.797

6.371 ± 0.876

6.216 ± 0.867

5.672 ± 1.646

6.138 ± 0.938

1.243 ± 0.316

4.662 ± 0.821
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski