Citrobacter phage CVT22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Citrovirus; Citrobacter virus CVT22

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R6CQ96|A0A0R6CQ96_9CAUD Uncharacterized protein OS=Citrobacter phage CVT22 OX=1622234 PE=4 SV=1
MM1 pKa = 7.31FNVGDD6 pKa = 4.08VVEE9 pKa = 5.02LDD11 pKa = 4.37LDD13 pKa = 4.24AEE15 pKa = 4.62VNNYY19 pKa = 8.26WGGIADD25 pKa = 4.23EE26 pKa = 4.36IKK28 pKa = 10.49YY29 pKa = 10.9LEE31 pKa = 4.27TFGLIPHH38 pKa = 6.33TAYY41 pKa = 10.33VVEE44 pKa = 4.82HH45 pKa = 6.96IEE47 pKa = 4.04YY48 pKa = 9.82DD49 pKa = 3.18WADD52 pKa = 3.36AEE54 pKa = 4.41EE55 pKa = 5.29GEE57 pKa = 4.73EE58 pKa = 4.11YY59 pKa = 10.28PPLVQLEE66 pKa = 4.38GVKK69 pKa = 10.41LDD71 pKa = 3.5WSADD75 pKa = 3.58RR76 pKa = 11.84FKK78 pKa = 10.78PASPLNLEE86 pKa = 4.28NEE88 pKa = 4.33

Molecular weight: 10.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R6C6Q1|A0A0R6C6Q1_9CAUD Uncharacterized protein OS=Citrobacter phage CVT22 OX=1622234 PE=4 SV=1
MM1 pKa = 7.18SHH3 pKa = 6.34KK4 pKa = 9.55QAKK7 pKa = 9.64RR8 pKa = 11.84LRR10 pKa = 11.84KK11 pKa = 9.34DD12 pKa = 3.33LNIVRR17 pKa = 11.84EE18 pKa = 4.19DD19 pKa = 3.29TQYY22 pKa = 10.19TDD24 pKa = 2.74KK25 pKa = 11.32VMRR28 pKa = 11.84TVMVDD33 pKa = 2.69TGRR36 pKa = 11.84IDD38 pKa = 4.15DD39 pKa = 4.38KK40 pKa = 11.43GNAIMRR46 pKa = 11.84PEE48 pKa = 3.89VRR50 pKa = 11.84TMRR53 pKa = 11.84EE54 pKa = 3.97CKK56 pKa = 10.38GEE58 pKa = 3.93RR59 pKa = 11.84FLYY62 pKa = 10.27QLLKK66 pKa = 10.41RR67 pKa = 11.84GRR69 pKa = 11.84SHH71 pKa = 6.96VLGGVV76 pKa = 3.17

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

14897

40

1573

179.5

20.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.357 ± 0.397

1.007 ± 0.175

6.135 ± 0.218

6.941 ± 0.349

3.873 ± 0.206

7.263 ± 0.279

1.886 ± 0.187

5.652 ± 0.263

7.169 ± 0.378

7.525 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.907 ± 0.153

5.216 ± 0.184

3.343 ± 0.184

3.652 ± 0.28

4.417 ± 0.204

6.095 ± 0.397

5.639 ± 0.329

7.27 ± 0.238

1.705 ± 0.144

3.947 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski