Frankia symbiont of Alnus alnobetula

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia; unclassified Frankia

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S5EU67|A0A4S5EU67_9ACTN Rrf2 family transcriptional regulator OS=Frankia symbiont of Alnus alnobetula OX=2699483 GN=E7Y31_01555 PE=4 SV=1
MM1 pKa = 6.31VTEE4 pKa = 4.7DD5 pKa = 4.01RR6 pKa = 11.84SPVATPAKK14 pKa = 9.19PGVSAGLRR22 pKa = 11.84VRR24 pKa = 11.84VDD26 pKa = 3.25QDD28 pKa = 2.97ACTGDD33 pKa = 3.84GLCVQLAPSVFEE45 pKa = 4.03FDD47 pKa = 3.48VDD49 pKa = 3.27GLAYY53 pKa = 10.75VKK55 pKa = 10.74DD56 pKa = 4.09DD57 pKa = 3.87AGEE60 pKa = 4.01LQTTGGAYY68 pKa = 10.32VPVPLPLVDD77 pKa = 4.2AVVDD81 pKa = 4.13AADD84 pKa = 4.25EE85 pKa = 4.46CPGTCIYY92 pKa = 10.67VEE94 pKa = 4.47RR95 pKa = 11.84PDD97 pKa = 3.53GTLEE101 pKa = 4.16AGGPLGG107 pKa = 4.52

Molecular weight: 10.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V6S8E9|A0A4V6S8E9_9ACTN Helix-turn-helix domain-containing protein OS=Frankia symbiont of Alnus alnobetula OX=2699483 GN=E7Y31_08220 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3955

0

3955

1112130

25

2172

281.2

29.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.284 ± 0.055

0.797 ± 0.011

6.129 ± 0.033

4.969 ± 0.031

2.588 ± 0.024

9.645 ± 0.04

2.175 ± 0.019

3.555 ± 0.028

1.511 ± 0.023

10.253 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.616 ± 0.013

1.6 ± 0.018

6.433 ± 0.033

2.591 ± 0.021

8.748 ± 0.046

5.134 ± 0.026

5.966 ± 0.035

8.76 ± 0.04

1.399 ± 0.018

1.846 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski