Bacillus coagulans 36D1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Weizmannia; Weizmannia coagulans

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2TLD3|G2TLD3_BACCO 50S ribosomal subunit assembly factor BipA OS=Bacillus coagulans 36D1 OX=345219 GN=bipA PE=3 SV=1
MM1 pKa = 7.8EE2 pKa = 4.87EE3 pKa = 4.57LKK5 pKa = 11.02LSEE8 pKa = 4.05QDD10 pKa = 4.87LINAVCTFVADD21 pKa = 2.98QHH23 pKa = 5.77YY24 pKa = 10.14AYY26 pKa = 9.0PEE28 pKa = 3.8NVMAMLTYY36 pKa = 10.98DD37 pKa = 4.45DD38 pKa = 4.06DD39 pKa = 4.0TGYY42 pKa = 11.18GADD45 pKa = 3.53VEE47 pKa = 5.61LNGKK51 pKa = 8.82DD52 pKa = 4.14LEE54 pKa = 4.58PLTEE58 pKa = 4.49PKK60 pKa = 10.3LIQAIRR66 pKa = 11.84KK67 pKa = 8.39YY68 pKa = 10.88LEE70 pKa = 3.91EE71 pKa = 4.92EE72 pKa = 4.15YY73 pKa = 10.84SIDD76 pKa = 3.5PFAVSIRR83 pKa = 11.84LEE85 pKa = 4.13IEE87 pKa = 4.25DD88 pKa = 3.53QDD90 pKa = 4.26GMVAYY95 pKa = 10.66VDD97 pKa = 3.57

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2TQG4|G2TQG4_BACCO Aldo/keto reductase OS=Bacillus coagulans 36D1 OX=345219 GN=Bcoa_0589 PE=4 SV=1
MM1 pKa = 7.69ILQITFSAIVYY12 pKa = 9.04VPAKK16 pKa = 10.26GLLFSCLNGRR26 pKa = 11.84RR27 pKa = 11.84AFANSFLVDD36 pKa = 3.79RR37 pKa = 11.84LRR39 pKa = 11.84SPFWAFRR46 pKa = 11.84NIRR49 pKa = 11.84VKK51 pKa = 9.96TEE53 pKa = 4.15PIGMVEE59 pKa = 4.68SPAIPNCKK67 pKa = 9.81GFPSLHH73 pKa = 6.01LL74 pKa = 4.25

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3275

0

3275

954149

30

1520

291.3

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.24 ± 0.045

0.829 ± 0.012

4.87 ± 0.031

7.21 ± 0.059

4.537 ± 0.038

7.307 ± 0.037

2.183 ± 0.02

7.1 ± 0.039

7.127 ± 0.041

9.742 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.02

3.832 ± 0.03

3.892 ± 0.03

3.551 ± 0.023

4.661 ± 0.038

5.494 ± 0.038

5.225 ± 0.031

6.962 ± 0.034

1.027 ± 0.017

3.413 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski