Gracilibacillus halophilus YIM-C55.5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Gracilibacillus; Gracilibacillus halophilus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2968 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N4WQS4|N4WQS4_9BACI Spore germination protein QC OS=Gracilibacillus halophilus YIM-C55.5 OX=1308866 GN=J416_08909 PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 10.23KK3 pKa = 9.82IYY5 pKa = 10.65SLILLLAISMFVVACGNDD23 pKa = 3.14SGGDD27 pKa = 3.51SEE29 pKa = 5.8ANAEE33 pKa = 4.15AEE35 pKa = 4.33GNEE38 pKa = 4.4TEE40 pKa = 4.28NQEE43 pKa = 4.16QQEE46 pKa = 4.56EE47 pKa = 4.36EE48 pKa = 4.4TPAEE52 pKa = 3.87IEE54 pKa = 4.28VEE56 pKa = 4.21HH57 pKa = 6.58EE58 pKa = 4.26LDD60 pKa = 3.42TTTVPTNPEE69 pKa = 3.78NVVVFDD75 pKa = 3.77MGTLEE80 pKa = 4.21TLDD83 pKa = 3.68EE84 pKa = 4.94LGVDD88 pKa = 3.85VAAVPKK94 pKa = 10.54EE95 pKa = 3.85NLPPHH100 pKa = 6.84LSQYY104 pKa = 11.5DD105 pKa = 3.3SDD107 pKa = 3.96EE108 pKa = 4.2YY109 pKa = 11.65KK110 pKa = 10.83NAGTLFEE117 pKa = 5.55PDD119 pKa = 3.96FEE121 pKa = 4.64TLATVDD127 pKa = 3.92PDD129 pKa = 4.37LIIISGRR136 pKa = 11.84TSEE139 pKa = 4.32VYY141 pKa = 10.52EE142 pKa = 4.68DD143 pKa = 3.9LAEE146 pKa = 4.26LAPTIYY152 pKa = 10.01MGVDD156 pKa = 3.02TEE158 pKa = 4.95NYY160 pKa = 8.8MEE162 pKa = 4.37SFEE165 pKa = 5.73SNVTTLGQIFTKK177 pKa = 10.39EE178 pKa = 3.96DD179 pKa = 3.41EE180 pKa = 4.59AEE182 pKa = 4.08QALTEE187 pKa = 4.28INDD190 pKa = 4.19SITALQEE197 pKa = 3.69KK198 pKa = 9.76ADD200 pKa = 3.69AMEE203 pKa = 4.59GNGLVILTNEE213 pKa = 4.3GGVSAFGAGSRR224 pKa = 11.84FGIIHH229 pKa = 7.09DD230 pKa = 3.82VFGVPEE236 pKa = 4.52ADD238 pKa = 3.34EE239 pKa = 5.13SIDD242 pKa = 4.39VSTHH246 pKa = 4.08GQNVSFEE253 pKa = 4.39YY254 pKa = 10.59LAEE257 pKa = 4.21TNPDD261 pKa = 3.43YY262 pKa = 11.49LFVIDD267 pKa = 4.63RR268 pKa = 11.84NTVVGGEE275 pKa = 3.96ASAEE279 pKa = 3.91KK280 pKa = 10.03TLDD283 pKa = 3.52NEE285 pKa = 4.7LVNEE289 pKa = 4.24TTAAQNDD296 pKa = 4.02QITYY300 pKa = 9.96LDD302 pKa = 3.67PYY304 pKa = 8.24YY305 pKa = 10.01WYY307 pKa = 10.92VSGGGLTSVKK317 pKa = 10.5EE318 pKa = 4.1MVNEE322 pKa = 3.88VSEE325 pKa = 4.21GLEE328 pKa = 3.87

Molecular weight: 35.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N4WVQ3|N4WVQ3_9BACI Glycosyl transferase OS=Gracilibacillus halophilus YIM-C55.5 OX=1308866 GN=J416_06937 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.2NGRR28 pKa = 11.84KK29 pKa = 9.11VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.99KK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2968

0

2968

838553

26

2311

282.5

31.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.496 ± 0.042

0.648 ± 0.012

5.832 ± 0.045

7.568 ± 0.054

4.49 ± 0.04

6.316 ± 0.046

2.443 ± 0.029

7.857 ± 0.051

5.978 ± 0.041

9.159 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.909 ± 0.024

4.509 ± 0.032

3.452 ± 0.027

4.954 ± 0.048

3.993 ± 0.031

5.969 ± 0.038

5.698 ± 0.031

6.913 ± 0.035

1.081 ± 0.019

3.736 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski