Lysobacter sp. HDW10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; unclassified Lysobacter

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7YVE6|A0A6G7YVE6_9GAMM Tryptophan synthase OS=Lysobacter sp. HDW10 OX=2714936 GN=trpA PE=3 SV=1
MM1 pKa = 7.57SDD3 pKa = 3.59EE4 pKa = 4.89NIALNGAEE12 pKa = 4.65APAEE16 pKa = 4.05ATGATFMIEE25 pKa = 3.93KK26 pKa = 9.83IYY28 pKa = 10.63TRR30 pKa = 11.84DD31 pKa = 2.97ISFEE35 pKa = 4.25VPNAPQVFTEE45 pKa = 4.0QGQPEE50 pKa = 4.71LQMNLAQRR58 pKa = 11.84MGQIAEE64 pKa = 4.09NVYY67 pKa = 10.46EE68 pKa = 4.28VVLTVTITCTMNGKK82 pKa = 8.74NAYY85 pKa = 9.28LAEE88 pKa = 4.11VQQAGIFGLQGFDD101 pKa = 3.63AQGLDD106 pKa = 3.29AMLGAHH112 pKa = 7.02CPNILYY118 pKa = 9.31PYY120 pKa = 9.17ARR122 pKa = 11.84QSISDD127 pKa = 3.99MIVAGGFPPFYY138 pKa = 8.95MQPINFDD145 pKa = 3.27ALYY148 pKa = 10.37AQSLQQRR155 pKa = 11.84AEE157 pKa = 4.13GAPALADD164 pKa = 3.6GTEE167 pKa = 4.23TAGNAA172 pKa = 3.43

Molecular weight: 18.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7YWI6|A0A6G7YWI6_9GAMM M28 family peptidase OS=Lysobacter sp. HDW10 OX=2714936 GN=G7069_05530 PE=4 SV=1
MM1 pKa = 7.36PTLFTPLSAALGGLMIGLAVLVLYY25 pKa = 9.85FSLGRR30 pKa = 11.84IAGISGILNKK40 pKa = 10.45AIEE43 pKa = 4.58DD44 pKa = 3.52RR45 pKa = 11.84DD46 pKa = 3.66DD47 pKa = 4.48RR48 pKa = 11.84LWRR51 pKa = 11.84WVFLVALMIGAVAMFKK67 pKa = 10.85VFDD70 pKa = 4.18LRR72 pKa = 11.84AGNAVVSMPWLIAAGLLVGAGTRR95 pKa = 11.84LGNGCTSGHH104 pKa = 6.8GICGLARR111 pKa = 11.84FSKK114 pKa = 10.25RR115 pKa = 11.84SAVAVAVFMATGMLTVYY132 pKa = 10.0VLRR135 pKa = 11.84HH136 pKa = 5.61ALWGGAA142 pKa = 3.45

Molecular weight: 14.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2055

0

2055

676245

41

5820

329.1

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.066 ± 0.066

0.855 ± 0.018

5.759 ± 0.033

5.487 ± 0.066

3.616 ± 0.041

7.935 ± 0.068

2.296 ± 0.03

4.903 ± 0.037

3.838 ± 0.053

9.966 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.029

3.096 ± 0.056

4.743 ± 0.041

3.688 ± 0.035

6.659 ± 0.066

5.716 ± 0.062

5.39 ± 0.082

7.668 ± 0.044

1.429 ± 0.023

2.289 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski