Salmonella phage vB_SenM-S16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Gelderlandvirus; Salmonella virus S16

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1HDH6|M1HDH6_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenM-S16 OX=1087482 PE=4 SV=1
MM1 pKa = 7.1STATCTSTNIDD12 pKa = 3.4VFRR15 pKa = 11.84LFEE18 pKa = 4.56EE19 pKa = 4.68YY20 pKa = 10.94EE21 pKa = 3.95MTKK24 pKa = 9.94ISEE27 pKa = 4.42NIISVMSDD35 pKa = 2.68TDD37 pKa = 3.93VEE39 pKa = 4.41WFIPLYY45 pKa = 11.0DD46 pKa = 3.89EE47 pKa = 4.5DD48 pKa = 4.49TYY50 pKa = 11.82EE51 pKa = 4.07VTDD54 pKa = 3.97ANGNKK59 pKa = 9.93AVFVIDD65 pKa = 3.53

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1HDI5|M1HDI5_9CAUD RI lysis inhibition regulator OS=Salmonella phage vB_SenM-S16 OX=1087482 PE=4 SV=1
MM1 pKa = 6.32NTKK4 pKa = 9.95IIHH7 pKa = 6.36DD8 pKa = 4.02KK9 pKa = 10.94KK10 pKa = 11.14LDD12 pKa = 3.78GSCIWKK18 pKa = 9.37FVISNKK24 pKa = 10.32DD25 pKa = 3.28GTFTTVLSVSKK36 pKa = 8.8LTPRR40 pKa = 11.84LIRR43 pKa = 11.84RR44 pKa = 11.84YY45 pKa = 10.27KK46 pKa = 10.35RR47 pKa = 11.84INRR50 pKa = 11.84LFNFF54 pKa = 4.23

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

268

0

268

50790

29

1273

189.5

21.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.385 ± 0.191

1.132 ± 0.075

6.214 ± 0.128

7.216 ± 0.153

4.328 ± 0.106

6.129 ± 0.192

1.776 ± 0.081

7.334 ± 0.134

7.726 ± 0.216

7.545 ± 0.11

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.098

5.792 ± 0.12

3.386 ± 0.104

3.262 ± 0.094

4.426 ± 0.101

6.523 ± 0.139

5.729 ± 0.211

6.511 ± 0.145

1.516 ± 0.069

4.184 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski