Butyrivibrio hungatei

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3003 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D9NYN1|A0A1D9NYN1_9FIRM RNA polymerase sigma factor sigma-70 family OS=Butyrivibrio hungatei OX=185008 GN=bhn_I0328 PE=3 SV=1
MM1 pKa = 7.15STHH4 pKa = 6.61KK5 pKa = 10.86LIDD8 pKa = 3.55KK9 pKa = 10.05VCIFVIIFAVLATVLFMNGSAIGITADD36 pKa = 3.76DD37 pKa = 4.19SVEE40 pKa = 3.84MGYY43 pKa = 10.74EE44 pKa = 3.75EE45 pKa = 6.05LLFDD49 pKa = 4.83DD50 pKa = 5.48SYY52 pKa = 12.03VHH54 pKa = 6.9TIDD57 pKa = 5.6IEE59 pKa = 4.59IDD61 pKa = 3.3DD62 pKa = 4.18WDD64 pKa = 4.32SFLEE68 pKa = 4.25TATSEE73 pKa = 4.44EE74 pKa = 4.24YY75 pKa = 10.59SAANVTIDD83 pKa = 3.61GEE85 pKa = 4.59TVYY88 pKa = 11.19NVGIRR93 pKa = 11.84GKK95 pKa = 10.95GNTSLSTVEE104 pKa = 4.34QLDD107 pKa = 3.37SDD109 pKa = 4.04RR110 pKa = 11.84YY111 pKa = 8.64SFKK114 pKa = 10.7IEE116 pKa = 3.73FDD118 pKa = 3.97CYY120 pKa = 10.23EE121 pKa = 4.1SSNTYY126 pKa = 10.17HH127 pKa = 7.22GLDD130 pKa = 3.66KK131 pKa = 11.32LCLNNLIQDD140 pKa = 3.77YY141 pKa = 9.67TMMKK145 pKa = 10.41DD146 pKa = 3.3YY147 pKa = 10.95LAYY150 pKa = 10.07KK151 pKa = 10.57LMGEE155 pKa = 4.66FGVDD159 pKa = 3.43APLVSYY165 pKa = 10.13VYY167 pKa = 9.28ITVNGEE173 pKa = 3.55DD174 pKa = 3.09WGLYY178 pKa = 9.37LAVEE182 pKa = 4.19AVEE185 pKa = 5.54DD186 pKa = 4.34SFLEE190 pKa = 4.32RR191 pKa = 11.84NNGTNYY197 pKa = 10.5GNLYY201 pKa = 10.55KK202 pKa = 10.15PDD204 pKa = 3.97TLSMGGGRR212 pKa = 11.84GNGRR216 pKa = 11.84DD217 pKa = 3.46FDD219 pKa = 4.39MNDD222 pKa = 3.06VDD224 pKa = 5.2SSDD227 pKa = 3.55MPEE230 pKa = 4.36GFGKK234 pKa = 10.71KK235 pKa = 9.79PGDD238 pKa = 3.45RR239 pKa = 11.84SGEE242 pKa = 4.03LPGKK246 pKa = 9.82DD247 pKa = 3.49QEE249 pKa = 4.48EE250 pKa = 4.31HH251 pKa = 6.94GGMGSDD257 pKa = 4.23DD258 pKa = 4.32SKK260 pKa = 11.54LKK262 pKa = 11.14YY263 pKa = 9.89IDD265 pKa = 5.48DD266 pKa = 5.91DD267 pKa = 3.62IDD269 pKa = 3.95SYY271 pKa = 12.23SNIFDD276 pKa = 3.75SAKK279 pKa = 10.3TNIKK283 pKa = 10.49KK284 pKa = 9.98SDD286 pKa = 3.42KK287 pKa = 10.37KK288 pKa = 10.98RR289 pKa = 11.84LINSLKK295 pKa = 9.22TLSEE299 pKa = 4.61GIEE302 pKa = 3.95EE303 pKa = 3.92QDD305 pKa = 2.98ADD307 pKa = 4.48KK308 pKa = 10.83IAEE311 pKa = 4.3AVDD314 pKa = 3.13VDD316 pKa = 3.37AVMRR320 pKa = 11.84YY321 pKa = 7.49MVVHH325 pKa = 6.34NFLVNGDD332 pKa = 4.21SYY334 pKa = 10.44TGSMIHH340 pKa = 6.56NYY342 pKa = 9.82YY343 pKa = 10.51LYY345 pKa = 10.82EE346 pKa = 4.33EE347 pKa = 5.53DD348 pKa = 5.12GLLSMIPWDD357 pKa = 3.61YY358 pKa = 11.46NLAYY362 pKa = 9.32GTFQGQDD369 pKa = 2.49ADD371 pKa = 4.13GAVNDD376 pKa = 5.66PIDD379 pKa = 3.87TPLSVSEE386 pKa = 5.06DD387 pKa = 3.39SDD389 pKa = 3.91DD390 pKa = 4.37RR391 pKa = 11.84PMFSWITSCDD401 pKa = 3.57EE402 pKa = 3.91YY403 pKa = 11.15TEE405 pKa = 4.72MYY407 pKa = 9.56HH408 pKa = 7.2EE409 pKa = 4.81YY410 pKa = 10.71YY411 pKa = 11.03ADD413 pKa = 3.7FLEE416 pKa = 4.05QFYY419 pKa = 10.46TSGYY423 pKa = 9.15LQDD426 pKa = 5.61LIEE429 pKa = 4.38STYY432 pKa = 11.85DD433 pKa = 3.28MISEE437 pKa = 4.24YY438 pKa = 10.61VEE440 pKa = 4.02KK441 pKa = 10.73DD442 pKa = 2.98PTKK445 pKa = 10.56FCTTEE450 pKa = 3.74EE451 pKa = 4.29FEE453 pKa = 4.61TAVEE457 pKa = 4.19TMKK460 pKa = 10.75TFVEE464 pKa = 4.69LRR466 pKa = 11.84CQSISGQLDD475 pKa = 3.42GTIGSTDD482 pKa = 3.28AEE484 pKa = 4.25QEE486 pKa = 4.27EE487 pKa = 4.69NPDD490 pKa = 3.68ALIDD494 pKa = 3.97ASSITLSDD502 pKa = 3.68MGSMGGGMGGGPGQGDD518 pKa = 4.45GEE520 pKa = 4.44HH521 pKa = 6.66SGPPSGDD528 pKa = 3.2PDD530 pKa = 3.73QNSDD534 pKa = 3.13GG535 pKa = 3.99

Molecular weight: 59.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D9P5J9|A0A1D9P5J9_9FIRM Sortase family protein OS=Butyrivibrio hungatei OX=185008 GN=bhn_II020 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 8.74MTFQPHH8 pKa = 5.93KK9 pKa = 8.68LQRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.36GGRR28 pKa = 11.84KK29 pKa = 8.97VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3003

0

3003

1018659

29

2445

339.2

38.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.199 ± 0.04

1.368 ± 0.019

6.509 ± 0.044

7.304 ± 0.05

4.412 ± 0.036

6.827 ± 0.038

1.612 ± 0.018

7.78 ± 0.043

7.277 ± 0.038

8.46 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.027 ± 0.023

4.771 ± 0.028

3.091 ± 0.026

2.648 ± 0.024

3.797 ± 0.027

6.233 ± 0.042

5.34 ± 0.039

6.956 ± 0.032

0.883 ± 0.015

4.506 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski