Amino acid dipepetide frequency for Noviherbaspirillum humi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.818AlaAla: 16.818 ± 0.149
1.31AlaCys: 1.31 ± 0.032
6.424AlaAsp: 6.424 ± 0.067
6.695AlaGlu: 6.695 ± 0.074
4.137AlaPhe: 4.137 ± 0.06
10.621AlaGly: 10.621 ± 0.106
2.289AlaHis: 2.289 ± 0.041
5.779AlaIle: 5.779 ± 0.071
4.312AlaLys: 4.312 ± 0.072
13.149AlaLeu: 13.149 ± 0.118
3.515AlaMet: 3.515 ± 0.053
3.294AlaAsn: 3.294 ± 0.06
5.729AlaPro: 5.729 ± 0.086
4.958AlaGln: 4.958 ± 0.067
8.216AlaArg: 8.216 ± 0.086
7.227AlaSer: 7.227 ± 0.099
5.396AlaThr: 5.396 ± 0.073
8.249AlaVal: 8.249 ± 0.08
1.626AlaTrp: 1.626 ± 0.037
2.586AlaTyr: 2.586 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.071CysAla: 1.071 ± 0.026
0.125CysCys: 0.125 ± 0.01
0.557CysAsp: 0.557 ± 0.018
0.484CysGlu: 0.484 ± 0.019
0.338CysPhe: 0.338 ± 0.015
0.968CysGly: 0.968 ± 0.03
0.259CysHis: 0.259 ± 0.013
0.436CysIle: 0.436 ± 0.018
0.236CysLys: 0.236 ± 0.014
0.866CysLeu: 0.866 ± 0.025
0.227CysMet: 0.227 ± 0.013
0.265CysAsn: 0.265 ± 0.012
0.447CysPro: 0.447 ± 0.021
0.257CysGln: 0.257 ± 0.012
0.649CysArg: 0.649 ± 0.019
0.529CysSer: 0.529 ± 0.02
0.396CysThr: 0.396 ± 0.016
0.614CysVal: 0.614 ± 0.02
0.125CysTrp: 0.125 ± 0.009
0.235CysTyr: 0.235 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.955AspAla: 6.955 ± 0.07
0.471AspCys: 0.471 ± 0.017
2.78AspAsp: 2.78 ± 0.045
3.126AspGlu: 3.126 ± 0.052
2.123AspPhe: 2.123 ± 0.029
4.561AspGly: 4.561 ± 0.059
1.064AspHis: 1.064 ± 0.026
2.995AspIle: 2.995 ± 0.04
1.957AspLys: 1.957 ± 0.046
5.171AspLeu: 5.171 ± 0.069
1.411AspMet: 1.411 ± 0.034
1.429AspAsn: 1.429 ± 0.033
2.824AspPro: 2.824 ± 0.049
1.655AspGln: 1.655 ± 0.032
3.277AspArg: 3.277 ± 0.048
2.368AspSer: 2.368 ± 0.039
2.398AspThr: 2.398 ± 0.042
3.819AspVal: 3.819 ± 0.057
0.869AspTrp: 0.869 ± 0.025
1.513AspTyr: 1.513 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.032GluAla: 7.032 ± 0.077
0.399GluCys: 0.399 ± 0.017
2.367GluAsp: 2.367 ± 0.04
3.28GluGlu: 3.28 ± 0.056
1.93GluPhe: 1.93 ± 0.034
3.669GluGly: 3.669 ± 0.061
1.211GluHis: 1.211 ± 0.031
3.052GluIle: 3.052 ± 0.05
2.592GluLys: 2.592 ± 0.051
5.661GluLeu: 5.661 ± 0.071
1.491GluMet: 1.491 ± 0.033
1.687GluAsn: 1.687 ± 0.034
2.366GluPro: 2.366 ± 0.042
2.849GluGln: 2.849 ± 0.048
4.636GluArg: 4.636 ± 0.065
2.804GluSer: 2.804 ± 0.047
2.631GluThr: 2.631 ± 0.045
3.879GluVal: 3.879 ± 0.061
0.701GluTrp: 0.701 ± 0.021
1.112GluTyr: 1.112 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.114PheAla: 4.114 ± 0.05
0.415PheCys: 0.415 ± 0.016
2.425PheAsp: 2.425 ± 0.04
1.967PheGlu: 1.967 ± 0.037
1.441PhePhe: 1.441 ± 0.035
3.406PheGly: 3.406 ± 0.055
0.765PheHis: 0.765 ± 0.023
1.899PheIle: 1.899 ± 0.04
1.21PheLys: 1.21 ± 0.028
3.18PheLeu: 3.18 ± 0.049
0.843PheMet: 0.843 ± 0.028
1.246PheAsn: 1.246 ± 0.029
1.657PhePro: 1.657 ± 0.033
1.099PheGln: 1.099 ± 0.03
2.123PheArg: 2.123 ± 0.038
2.468PheSer: 2.468 ± 0.045
1.898PheThr: 1.898 ± 0.041
2.536PheVal: 2.536 ± 0.042
0.484PheTrp: 0.484 ± 0.021
0.987PheTyr: 0.987 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
8.633GlyAla: 8.633 ± 0.091
0.885GlyCys: 0.885 ± 0.029
3.934GlyAsp: 3.934 ± 0.059
4.349GlyGlu: 4.349 ± 0.047
3.298GlyPhe: 3.298 ± 0.048
6.832GlyGly: 6.832 ± 0.102
1.82GlyHis: 1.82 ± 0.036
4.603GlyIle: 4.603 ± 0.065
3.854GlyLys: 3.854 ± 0.06
8.083GlyLeu: 8.083 ± 0.084
2.699GlyMet: 2.699 ± 0.042
2.701GlyAsn: 2.701 ± 0.071
2.7GlyPro: 2.7 ± 0.041
3.046GlyGln: 3.046 ± 0.047
5.469GlyArg: 5.469 ± 0.068
4.861GlySer: 4.861 ± 0.08
4.492GlyThr: 4.492 ± 0.086
5.727GlyVal: 5.727 ± 0.063
1.207GlyTrp: 1.207 ± 0.029
2.361GlyTyr: 2.361 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.767HisAla: 2.767 ± 0.047
0.273HisCys: 0.273 ± 0.013
1.22HisAsp: 1.22 ± 0.028
1.182HisGlu: 1.182 ± 0.033
0.884HisPhe: 0.884 ± 0.025
2.052HisGly: 2.052 ± 0.042
0.631HisHis: 0.631 ± 0.026
0.976HisIle: 0.976 ± 0.023
0.569HisLys: 0.569 ± 0.021
2.135HisLeu: 2.135 ± 0.045
0.503HisMet: 0.503 ± 0.018
0.509HisAsn: 0.509 ± 0.018
1.484HisPro: 1.484 ± 0.03
0.796HisGln: 0.796 ± 0.023
1.355HisArg: 1.355 ± 0.031
1.015HisSer: 1.015 ± 0.026
0.865HisThr: 0.865 ± 0.024
1.555HisVal: 1.555 ± 0.031
0.328HisTrp: 0.328 ± 0.016
0.613HisTyr: 0.613 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.599IleAla: 6.599 ± 0.071
0.49IleCys: 0.49 ± 0.019
3.562IleAsp: 3.562 ± 0.045
3.19IleGlu: 3.19 ± 0.047
1.491IlePhe: 1.491 ± 0.035
4.902IleGly: 4.902 ± 0.067
0.996IleHis: 0.996 ± 0.029
2.179IleIle: 2.179 ± 0.048
1.775IleLys: 1.775 ± 0.043
4.101IleLeu: 4.101 ± 0.057
0.976IleMet: 0.976 ± 0.023
1.736IleAsn: 1.736 ± 0.041
2.295IlePro: 2.295 ± 0.038
1.449IleGln: 1.449 ± 0.029
3.076IleArg: 3.076 ± 0.052
2.938IleSer: 2.938 ± 0.044
2.745IleThr: 2.745 ± 0.056
3.629IleVal: 3.629 ± 0.049
0.508IleTrp: 0.508 ± 0.019
1.116IleTyr: 1.116 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
4.303LysAla: 4.303 ± 0.068
0.191LysCys: 0.191 ± 0.01
1.796LysAsp: 1.796 ± 0.039
2.022LysGlu: 2.022 ± 0.043
1.07LysPhe: 1.07 ± 0.027
2.455LysGly: 2.455 ± 0.043
0.739LysHis: 0.739 ± 0.023
1.777LysIle: 1.777 ± 0.036
1.65LysLys: 1.65 ± 0.05
4.011LysLeu: 4.011 ± 0.062
0.978LysMet: 0.978 ± 0.03
1.227LysAsn: 1.227 ± 0.03
2.55LysPro: 2.55 ± 0.043
1.613LysGln: 1.613 ± 0.034
2.541LysArg: 2.541 ± 0.043
2.055LysSer: 2.055 ± 0.037
2.11LysThr: 2.11 ± 0.041
2.729LysVal: 2.729 ± 0.048
0.4LysTrp: 0.4 ± 0.016
0.745LysTyr: 0.745 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
13.609LeuAla: 13.609 ± 0.123
1.001LeuCys: 1.001 ± 0.029
5.46LeuAsp: 5.46 ± 0.066
5.396LeuGlu: 5.396 ± 0.074
3.588LeuPhe: 3.588 ± 0.056
7.849LeuGly: 7.849 ± 0.094
2.236LeuHis: 2.236 ± 0.043
4.662LeuIle: 4.662 ± 0.065
3.973LeuLys: 3.973 ± 0.052
11.138LeuLeu: 11.138 ± 0.144
2.484LeuMet: 2.484 ± 0.039
3.108LeuAsn: 3.108 ± 0.049
6.073LeuPro: 6.073 ± 0.08
4.188LeuGln: 4.188 ± 0.055
7.063LeuArg: 7.063 ± 0.086
6.491LeuSer: 6.491 ± 0.079
5.157LeuThr: 5.157 ± 0.069
6.898LeuVal: 6.898 ± 0.077
1.081LeuTrp: 1.081 ± 0.034
2.141LeuTyr: 2.141 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
3.065MetAla: 3.065 ± 0.049
0.159MetCys: 0.159 ± 0.01
1.191MetAsp: 1.191 ± 0.028
1.43MetGlu: 1.43 ± 0.029
0.739MetPhe: 0.739 ± 0.025
1.75MetGly: 1.75 ± 0.036
0.603MetHis: 0.603 ± 0.02
1.156MetIle: 1.156 ± 0.027
1.242MetLys: 1.242 ± 0.026
2.984MetLeu: 2.984 ± 0.049
0.728MetMet: 0.728 ± 0.02
0.971MetAsn: 0.971 ± 0.023
1.666MetPro: 1.666 ± 0.033
1.308MetGln: 1.308 ± 0.032
1.864MetArg: 1.864 ± 0.038
1.604MetSer: 1.604 ± 0.037
1.552MetThr: 1.552 ± 0.03
1.695MetVal: 1.695 ± 0.037
0.224MetTrp: 0.224 ± 0.012
0.436MetTyr: 0.436 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.65AsnAla: 3.65 ± 0.061
0.295AsnCys: 0.295 ± 0.015
1.544AsnAsp: 1.544 ± 0.032
1.481AsnGlu: 1.481 ± 0.034
1.116AsnPhe: 1.116 ± 0.026
2.829AsnGly: 2.829 ± 0.074
0.602AsnHis: 0.602 ± 0.021
1.551AsnIle: 1.551 ± 0.037
0.969AsnLys: 0.969 ± 0.029
3.062AsnLeu: 3.062 ± 0.046
0.689AsnMet: 0.689 ± 0.02
1.014AsnAsn: 1.014 ± 0.036
2.016AsnPro: 2.016 ± 0.036
1.169AsnGln: 1.169 ± 0.028
2.091AsnArg: 2.091 ± 0.041
1.552AsnSer: 1.552 ± 0.045
1.521AsnThr: 1.521 ± 0.039
2.197AsnVal: 2.197 ± 0.049
0.443AsnTrp: 0.443 ± 0.018
0.827AsnTyr: 0.827 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
6.985ProAla: 6.985 ± 0.094
0.328ProCys: 0.328 ± 0.016
3.179ProAsp: 3.179 ± 0.05
3.456ProGlu: 3.456 ± 0.055
1.802ProPhe: 1.802 ± 0.035
4.341ProGly: 4.341 ± 0.056
1.089ProHis: 1.089 ± 0.026
2.142ProIle: 2.142 ± 0.038
1.715ProLys: 1.715 ± 0.035
4.882ProLeu: 4.882 ± 0.065
1.193ProMet: 1.193 ± 0.03
1.581ProAsn: 1.581 ± 0.035
2.487ProPro: 2.487 ± 0.051
2.106ProGln: 2.106 ± 0.039
2.7ProArg: 2.7 ± 0.041
2.884ProSer: 2.884 ± 0.057
2.392ProThr: 2.392 ± 0.096
4.205ProVal: 4.205 ± 0.059
0.639ProTrp: 0.639 ± 0.022
1.268ProTyr: 1.268 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
5.589GlnAla: 5.589 ± 0.059
0.299GlnCys: 0.299 ± 0.014
1.732GlnAsp: 1.732 ± 0.039
2.071GlnGlu: 2.071 ± 0.042
1.293GlnPhe: 1.293 ± 0.03
2.781GlnGly: 2.781 ± 0.044
0.926GlnHis: 0.926 ± 0.025
1.937GlnIle: 1.937 ± 0.042
1.377GlnLys: 1.377 ± 0.033
3.886GlnLeu: 3.886 ± 0.049
1.073GlnMet: 1.073 ± 0.025
1.065GlnAsn: 1.065 ± 0.028
2.277GlnPro: 2.277 ± 0.043
2.076GlnGln: 2.076 ± 0.047
3.139GlnArg: 3.139 ± 0.051
2.134GlnSer: 2.134 ± 0.034
1.88GlnThr: 1.88 ± 0.037
2.931GlnVal: 2.931 ± 0.046
0.507GlnTrp: 0.507 ± 0.017
0.839GlnTyr: 0.839 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
6.788ArgAla: 6.788 ± 0.08
0.551ArgCys: 0.551 ± 0.019
3.671ArgAsp: 3.671 ± 0.056
4.201ArgGlu: 4.201 ± 0.056
2.942ArgPhe: 2.942 ± 0.044
4.254ArgGly: 4.254 ± 0.059
1.945ArgHis: 1.945 ± 0.038
3.973ArgIle: 3.973 ± 0.056
2.41ArgLys: 2.41 ± 0.041
7.926ArgLeu: 7.926 ± 0.094
2.055ArgMet: 2.055 ± 0.036
2.119ArgAsn: 2.119 ± 0.042
3.03ArgPro: 3.03 ± 0.045
3.243ArgGln: 3.243 ± 0.05
5.323ArgArg: 5.323 ± 0.08
3.572ArgSer: 3.572 ± 0.057
2.947ArgThr: 2.947 ± 0.045
4.224ArgVal: 4.224 ± 0.055
0.982ArgTrp: 0.982 ± 0.028
1.894ArgTyr: 1.894 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.628SerAla: 6.628 ± 0.083
0.49SerCys: 0.49 ± 0.018
2.847SerAsp: 2.847 ± 0.041
2.784SerGlu: 2.784 ± 0.049
2.13SerPhe: 2.13 ± 0.042
5.666SerGly: 5.666 ± 0.087
1.236SerHis: 1.236 ± 0.027
2.815SerIle: 2.815 ± 0.047
1.808SerLys: 1.808 ± 0.039
6.168SerLeu: 6.168 ± 0.07
1.529SerMet: 1.529 ± 0.032
1.702SerAsn: 1.702 ± 0.042
2.911SerPro: 2.911 ± 0.047
2.06SerGln: 2.06 ± 0.039
3.793SerArg: 3.793 ± 0.061
3.55SerSer: 3.55 ± 0.067
2.914SerThr: 2.914 ± 0.057
4.011SerVal: 4.011 ± 0.053
0.722SerTrp: 0.722 ± 0.023
1.409SerTyr: 1.409 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
5.638ThrAla: 5.638 ± 0.083
0.388ThrCys: 0.388 ± 0.018
2.47ThrAsp: 2.47 ± 0.044
2.381ThrGlu: 2.381 ± 0.04
1.688ThrPhe: 1.688 ± 0.031
4.606ThrGly: 4.606 ± 0.08
1.025ThrHis: 1.025 ± 0.025
2.485ThrIle: 2.485 ± 0.048
1.27ThrLys: 1.27 ± 0.034
5.743ThrLeu: 5.743 ± 0.068
1.17ThrMet: 1.17 ± 0.03
1.399ThrAsn: 1.399 ± 0.035
3.265ThrPro: 3.265 ± 0.101
1.703ThrGln: 1.703 ± 0.038
3.067ThrArg: 3.067 ± 0.046
2.778ThrSer: 2.778 ± 0.062
2.594ThrThr: 2.594 ± 0.055
4.051ThrVal: 4.051 ± 0.058
0.616ThrTrp: 0.616 ± 0.025
1.214ThrTyr: 1.214 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
8.381ValAla: 8.381 ± 0.087
0.641ValCys: 0.641 ± 0.021
3.792ValAsp: 3.792 ± 0.054
4.058ValGlu: 4.058 ± 0.06
2.623ValPhe: 2.623 ± 0.044
5.09ValGly: 5.09 ± 0.062
1.411ValHis: 1.411 ± 0.032
3.725ValIle: 3.725 ± 0.054
2.791ValLys: 2.791 ± 0.044
7.153ValLeu: 7.153 ± 0.082
1.897ValMet: 1.897 ± 0.038
2.423ValAsn: 2.423 ± 0.049
3.78ValPro: 3.78 ± 0.057
2.486ValGln: 2.486 ± 0.045
4.662ValArg: 4.662 ± 0.069
4.299ValSer: 4.299 ± 0.056
3.866ValThr: 3.866 ± 0.059
5.66ValVal: 5.66 ± 0.071
0.822ValTrp: 0.822 ± 0.026
1.548ValTyr: 1.548 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.051TrpAla: 1.051 ± 0.029
0.126TrpCys: 0.126 ± 0.008
0.543TrpAsp: 0.543 ± 0.019
0.595TrpGlu: 0.595 ± 0.019
0.513TrpPhe: 0.513 ± 0.016
0.813TrpGly: 0.813 ± 0.025
0.363TrpHis: 0.363 ± 0.016
0.672TrpIle: 0.672 ± 0.022
0.494TrpLys: 0.494 ± 0.02
1.702TrpLeu: 1.702 ± 0.042
0.43TrpMet: 0.43 ± 0.016
0.457TrpAsn: 0.457 ± 0.019
0.584TrpPro: 0.584 ± 0.018
0.711TrpGln: 0.711 ± 0.023
1.107TrpArg: 1.107 ± 0.028
0.743TrpSer: 0.743 ± 0.022
0.64TrpThr: 0.64 ± 0.022
0.792TrpVal: 0.792 ± 0.023
0.19TrpTrp: 0.19 ± 0.014
0.312TrpTyr: 0.312 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.654TyrAla: 2.654 ± 0.047
0.243TyrCys: 0.243 ± 0.013
1.273TyrAsp: 1.273 ± 0.03
1.202TyrGlu: 1.202 ± 0.025
1.017TyrPhe: 1.017 ± 0.03
2.112TyrGly: 2.112 ± 0.041
0.501TyrHis: 0.501 ± 0.019
0.941TyrIle: 0.941 ± 0.024
0.757TyrLys: 0.757 ± 0.021
2.594TyrLeu: 2.594 ± 0.039
0.476TyrMet: 0.476 ± 0.018
0.682TyrAsn: 0.682 ± 0.023
1.234TyrPro: 1.234 ± 0.029
1.038TyrGln: 1.038 ± 0.024
1.899TyrArg: 1.899 ± 0.039
1.3TyrSer: 1.3 ± 0.031
1.155TyrThr: 1.155 ± 0.032
1.708TyrVal: 1.708 ± 0.038
0.367TyrTrp: 0.367 ± 0.019
0.679TyrTyr: 0.679 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4789 proteins (1549550 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski