Human smacovirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Huchismacovirus; unclassified Huchismacovirus

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5CVG9|A0A0B5CVG9_9VIRU Cap OS=Human smacovirus 1 OX=1595998 GN=Cap PE=4 SV=1
MM1 pKa = 6.74TTQSYY6 pKa = 8.28TYY8 pKa = 10.17FLDD11 pKa = 4.41LATSPDD17 pKa = 3.4KK18 pKa = 11.09VQVVGVSAGGMEE30 pKa = 4.57CMRR33 pKa = 11.84RR34 pKa = 11.84LLPFFAAYY42 pKa = 9.41KK43 pKa = 9.23YY44 pKa = 10.85YY45 pKa = 11.04KK46 pKa = 9.97LGHH49 pKa = 6.08VKK51 pKa = 10.57LKK53 pKa = 10.33VVPASTLPVDD63 pKa = 3.87PTGLSYY69 pKa = 10.68EE70 pKa = 4.23AGEE73 pKa = 4.26NTVDD77 pKa = 5.06PRR79 pKa = 11.84DD80 pKa = 3.46QFNPGLVRR88 pKa = 11.84ITNGEE93 pKa = 4.4DD94 pKa = 2.76IVVPTTMDD102 pKa = 2.85QYY104 pKa = 11.53YY105 pKa = 11.13SLMLDD110 pKa = 3.31RR111 pKa = 11.84RR112 pKa = 11.84WFKK115 pKa = 11.25FNLQSGFSRR124 pKa = 11.84DD125 pKa = 3.09AVPRR129 pKa = 11.84VWRR132 pKa = 11.84IAQLHH137 pKa = 5.29QDD139 pKa = 4.04RR140 pKa = 11.84FPGNTQNFPSVRR152 pKa = 11.84NPLRR156 pKa = 11.84EE157 pKa = 3.87VYY159 pKa = 9.97NRR161 pKa = 11.84QGLVSSADD169 pKa = 3.66PPAVEE174 pKa = 4.26YY175 pKa = 10.36TPLDD179 pKa = 3.73EE180 pKa = 5.93GSDD183 pKa = 3.51PRR185 pKa = 11.84GFFQVGNDD193 pKa = 4.0MYY195 pKa = 11.31LDD197 pKa = 3.92WLPTDD202 pKa = 5.23SDD204 pKa = 4.48TAWDD208 pKa = 3.87TTPNIPKK215 pKa = 9.46YY216 pKa = 9.96GRR218 pKa = 11.84PAAVPEE224 pKa = 4.32VPLMTFILPQAYY236 pKa = 6.99KK237 pKa = 8.99TRR239 pKa = 11.84FYY241 pKa = 10.27YY242 pKa = 9.77RR243 pKa = 11.84VYY245 pKa = 9.5ITEE248 pKa = 3.87TVYY251 pKa = 10.73FKK253 pKa = 11.25EE254 pKa = 4.03PVALNVSSVSSPGSFVVNNGVDD276 pKa = 3.48RR277 pKa = 11.84FIGLPDD283 pKa = 3.24IASGEE288 pKa = 4.11RR289 pKa = 11.84LSSIISASPVYY300 pKa = 10.16SGYY303 pKa = 10.56INDD306 pKa = 3.77GSDD309 pKa = 3.22YY310 pKa = 11.38RR311 pKa = 11.84EE312 pKa = 4.25DD313 pKa = 3.25TGGAA317 pKa = 3.39

Molecular weight: 35.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5CVG9|A0A0B5CVG9_9VIRU Cap OS=Human smacovirus 1 OX=1595998 GN=Cap PE=4 SV=1
MM1 pKa = 7.68TEE3 pKa = 4.27PKK5 pKa = 9.66WYY7 pKa = 10.02DD8 pKa = 3.0ITVSKK13 pKa = 10.62AKK15 pKa = 10.36CPEE18 pKa = 4.12EE19 pKa = 4.44ILRR22 pKa = 11.84KK23 pKa = 9.22WLDD26 pKa = 3.68EE27 pKa = 4.1NGEE30 pKa = 4.0RR31 pKa = 11.84YY32 pKa = 10.01AYY34 pKa = 10.18GRR36 pKa = 11.84EE37 pKa = 3.94RR38 pKa = 11.84GEE40 pKa = 4.2DD41 pKa = 3.78GYY43 pKa = 11.18EE44 pKa = 3.79HH45 pKa = 6.21FQVRR49 pKa = 11.84VVLRR53 pKa = 11.84NPTSWEE59 pKa = 3.79TMRR62 pKa = 11.84EE63 pKa = 3.68IWGHH67 pKa = 5.79SGHH70 pKa = 6.9CSPTSVRR77 pKa = 11.84NFDD80 pKa = 3.75YY81 pKa = 10.92VLKK84 pKa = 10.38EE85 pKa = 3.68GDD87 pKa = 5.32FICSWIKK94 pKa = 10.79VPDD97 pKa = 4.89AIRR100 pKa = 11.84NAQLRR105 pKa = 11.84PWQQHH110 pKa = 5.67LVDD113 pKa = 5.13LKK115 pKa = 10.9QNDD118 pKa = 3.97RR119 pKa = 11.84EE120 pKa = 4.07VDD122 pKa = 4.03CIIDD126 pKa = 3.68LRR128 pKa = 11.84GNTGKK133 pKa = 10.67SFVTKK138 pKa = 9.01YY139 pKa = 9.83MCMKK143 pKa = 10.56NMAINIPSGLTGKK156 pKa = 10.33DD157 pKa = 3.08IMRR160 pKa = 11.84MCMKK164 pKa = 10.13RR165 pKa = 11.84AKK167 pKa = 10.17LGTYY171 pKa = 9.84IFDD174 pKa = 3.61IPRR177 pKa = 11.84AGSKK181 pKa = 7.45QAKK184 pKa = 5.88EE185 pKa = 3.8TWSAIEE191 pKa = 4.3SIKK194 pKa = 10.51NGYY197 pKa = 7.86MWDD200 pKa = 3.89DD201 pKa = 3.64RR202 pKa = 11.84YY203 pKa = 11.19SWEE206 pKa = 4.16EE207 pKa = 3.53MWIEE211 pKa = 4.07SPRR214 pKa = 11.84VFVFTNEE221 pKa = 3.8YY222 pKa = 9.62PKK224 pKa = 10.97YY225 pKa = 10.4DD226 pKa = 4.21LLSEE230 pKa = 4.02DD231 pKa = 4.74RR232 pKa = 11.84FRR234 pKa = 11.84FWAPGEE240 pKa = 4.14LGLVQLAKK248 pKa = 10.77

Molecular weight: 29.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

565

248

317

282.5

32.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.133 ± 0.464

1.239 ± 0.786

6.903 ± 0.3

5.664 ± 1.598

4.425 ± 0.53

6.903 ± 0.3

1.062 ± 0.367

4.779 ± 1.113

4.779 ± 1.65

6.726 ± 0.451

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.009 ± 0.681

4.248 ± 0.143

6.726 ± 1.524

3.186 ± 0.242

6.903 ± 0.505

6.903 ± 0.837

5.664 ± 0.817

7.611 ± 1.577

2.655 ± 1.185

5.487 ± 0.7
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski