Escherichia virus AAPEc6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Vectrevirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BK47|A0A1P8BK47_9CAUD Uncharacterized protein OS=Escherichia virus AAPEc6 OX=1873778 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 9.84SQVYY12 pKa = 7.34NTAHH16 pKa = 6.61AALVEE21 pKa = 4.35RR22 pKa = 11.84VWDD25 pKa = 4.14DD26 pKa = 5.48AIDD29 pKa = 4.95CIDD32 pKa = 3.8NAHH35 pKa = 6.71DD36 pKa = 5.38AIHH39 pKa = 6.31EE40 pKa = 4.45VADD43 pKa = 3.79SAVPIYY49 pKa = 10.46YY50 pKa = 10.45ADD52 pKa = 3.63IFSVMASEE60 pKa = 5.34GIDD63 pKa = 3.4HH64 pKa = 6.92FFEE67 pKa = 6.26DD68 pKa = 4.36SGLMPDD74 pKa = 3.68TKK76 pKa = 10.96DD77 pKa = 3.3VTVVLQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.14QLTIDD93 pKa = 3.14LWEE96 pKa = 4.59RR97 pKa = 11.84VQDD100 pKa = 4.14MIDD103 pKa = 3.46AYY105 pKa = 9.93IDD107 pKa = 3.48SAEE110 pKa = 4.12DD111 pKa = 3.45SEE113 pKa = 4.75EE114 pKa = 4.12EE115 pKa = 4.17EE116 pKa = 4.44EE117 pKa = 4.34

Molecular weight: 13.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BK48|A0A1P8BK48_9CAUD Uncharacterized protein OS=Escherichia virus AAPEc6 OX=1873778 PE=4 SV=1
MM1 pKa = 7.71ACLSLLRR8 pKa = 11.84RR9 pKa = 11.84NKK11 pKa = 9.35KK12 pKa = 9.38RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.39KK17 pKa = 10.08VKK19 pKa = 10.47RR20 pKa = 11.84IMLVTILLIGSLTTSGCQQALPEE43 pKa = 4.05KK44 pKa = 11.05AMVKK48 pKa = 9.82PLRR51 pKa = 11.84PTLTEE56 pKa = 3.95VYY58 pKa = 10.07EE59 pKa = 4.36VDD61 pKa = 5.33DD62 pKa = 4.54KK63 pKa = 12.16VCFSKK68 pKa = 10.9PDD70 pKa = 3.35ATKK73 pKa = 10.5LGLYY77 pKa = 9.72ILSLEE82 pKa = 4.16RR83 pKa = 11.84GYY85 pKa = 11.36NN86 pKa = 3.32

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13721

37

1102

263.9

29.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.03 ± 0.476

1.057 ± 0.175

6.209 ± 0.224

7.208 ± 0.384

3.717 ± 0.135

7.704 ± 0.345

2.099 ± 0.239

5.32 ± 0.217

6.581 ± 0.37

7.718 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.039 ± 0.189

4.198 ± 0.21

3.549 ± 0.175

4.256 ± 0.26

5.43 ± 0.258

5.881 ± 0.391

5.459 ± 0.254

6.661 ± 0.266

1.407 ± 0.127

3.469 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski