Candidatus Aquiluna sp. UB-MaderosW2red

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Luna cluster; Luna-1 subcluster; Aquiluna; unclassified Aquiluna

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1613 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4VLI6|A0A1G4VLI6_9MICO Uncharacterized protein OS=Candidatus Aquiluna sp. UB-MaderosW2red OX=1855377 GN=SAMN05216534_0798 PE=4 SV=1
MM1 pKa = 7.52GKK3 pKa = 9.94YY4 pKa = 9.47LALGAAMLLTLSGCVVSSEE23 pKa = 4.33SGANEE28 pKa = 4.03PEE30 pKa = 4.19DD31 pKa = 3.95AANSAASEE39 pKa = 4.21SSSQQEE45 pKa = 4.15STSQGNGPWAVGEE58 pKa = 4.53LYY60 pKa = 10.51VASDD64 pKa = 3.33HH65 pKa = 5.83TVIINSVRR73 pKa = 11.84TTVEE77 pKa = 3.89SGFSEE82 pKa = 4.31PDD84 pKa = 2.71NDD86 pKa = 5.1FILLIDD92 pKa = 3.65MTIEE96 pKa = 3.77NTGNDD101 pKa = 3.47SHH103 pKa = 7.48SVSSIFGFEE112 pKa = 4.07LQGSDD117 pKa = 4.95DD118 pKa = 3.71YY119 pKa = 11.46MYY121 pKa = 11.04DD122 pKa = 3.03QDD124 pKa = 4.69YY125 pKa = 9.82FADD128 pKa = 3.77TKK130 pKa = 11.19GGLEE134 pKa = 4.12GEE136 pKa = 4.32IAPGSKK142 pKa = 10.28LRR144 pKa = 11.84GEE146 pKa = 4.23IAFDD150 pKa = 3.63VPEE153 pKa = 4.44LEE155 pKa = 4.97FYY157 pKa = 11.22SLLYY161 pKa = 10.83SHH163 pKa = 7.49DD164 pKa = 4.27LFADD168 pKa = 4.25SITFRR173 pKa = 11.84IAASDD178 pKa = 3.9LNIASSAAPEE188 pKa = 4.09EE189 pKa = 4.89SNSSVGLGVGDD200 pKa = 3.78TAEE203 pKa = 4.7GPGFTITLNSVRR215 pKa = 11.84SSEE218 pKa = 4.19EE219 pKa = 3.82DD220 pKa = 3.49SFSGPDD226 pKa = 3.09NDD228 pKa = 5.28FYY230 pKa = 11.59LILDD234 pKa = 3.59MTIEE238 pKa = 4.07NTSDD242 pKa = 3.21EE243 pKa = 4.41EE244 pKa = 4.45ASVSSLASFDD254 pKa = 4.58LKK256 pKa = 11.26GSDD259 pKa = 3.51LYY261 pKa = 10.97QYY263 pKa = 11.11SVGYY267 pKa = 9.09FADD270 pKa = 3.49VKK272 pKa = 11.17GDD274 pKa = 3.77LDD276 pKa = 3.85GSIPAGSKK284 pKa = 10.46LRR286 pKa = 11.84GEE288 pKa = 4.49VAFDD292 pKa = 3.43VPALSFYY299 pKa = 10.88EE300 pKa = 4.12FTYY303 pKa = 10.57KK304 pKa = 10.28HH305 pKa = 6.83DD306 pKa = 4.01FFGSSVTFLIDD317 pKa = 3.94EE318 pKa = 4.65SDD320 pKa = 3.38LGG322 pKa = 3.86

Molecular weight: 34.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4VC75|A0A1G4VC75_9MICO Chromosome partitioning protein OS=Candidatus Aquiluna sp. UB-MaderosW2red OX=1855377 GN=SAMN05216534_0263 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.34GFRR20 pKa = 11.84ARR22 pKa = 11.84MHH24 pKa = 5.46TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.65GRR40 pKa = 11.84SEE42 pKa = 3.94LSAA45 pKa = 4.73

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1613

0

1613

512337

25

7338

317.6

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.561 ± 0.067

0.536 ± 0.013

5.111 ± 0.043

6.218 ± 0.086

3.951 ± 0.042

8.219 ± 0.085

1.674 ± 0.031

6.245 ± 0.045

4.412 ± 0.057

10.804 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.149 ± 0.035

3.299 ± 0.05

4.414 ± 0.033

3.569 ± 0.034

5.211 ± 0.065

7.036 ± 0.084

5.453 ± 0.123

7.686 ± 0.057

1.242 ± 0.03

2.21 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski