Gordonia phage Hello

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Woesvirus; unclassified Woesvirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411B4S0|A0A411B4S0_9CAUD Uncharacterized protein OS=Gordonia phage Hello OX=2510573 GN=78 PE=4 SV=1
MM1 pKa = 8.06DD2 pKa = 4.54VGRR5 pKa = 11.84LMQGPPYY12 pKa = 9.94YY13 pKa = 10.58AGPEE17 pKa = 3.77EE18 pKa = 4.42LGYY21 pKa = 8.86YY22 pKa = 8.06TYY24 pKa = 10.35PDD26 pKa = 3.34SDD28 pKa = 3.95MDD30 pKa = 3.71EE31 pKa = 3.78LAYY34 pKa = 10.42RR35 pKa = 11.84IRR37 pKa = 11.84SRR39 pKa = 11.84LIPIWDD45 pKa = 3.8EE46 pKa = 4.39LEE48 pKa = 4.32VPLLPEE54 pKa = 3.8EE55 pKa = 4.3EE56 pKa = 4.73EE57 pKa = 3.94IVEE60 pKa = 4.09ATEE63 pKa = 4.01FF64 pKa = 3.55

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411B4L0|A0A411B4L0_9CAUD Uncharacterized protein OS=Gordonia phage Hello OX=2510573 GN=15 PE=4 SV=1
MM1 pKa = 6.96PQNRR5 pKa = 11.84RR6 pKa = 11.84NKK8 pKa = 9.37RR9 pKa = 11.84IQVRR13 pKa = 11.84VRR15 pKa = 11.84AEE17 pKa = 3.93QTDD20 pKa = 3.14GGYY23 pKa = 10.13IISVFDD29 pKa = 4.3RR30 pKa = 11.84AGLLAKK36 pKa = 8.5TEE38 pKa = 4.59CFHH41 pKa = 6.14KK42 pKa = 9.97TNVARR47 pKa = 11.84TAAQAASVALGVSEE61 pKa = 3.99GRR63 pKa = 11.84IKK65 pKa = 10.54ISPLEE70 pKa = 4.03YY71 pKa = 10.66AA72 pKa = 4.55

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

22607

33

3322

248.4

27.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.913 ± 1.054

0.942 ± 0.173

6.118 ± 0.332

6.971 ± 0.393

3.552 ± 0.121

7.98 ± 0.321

1.65 ± 0.202

5.357 ± 0.234

5.021 ± 0.209

7.29 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.442 ± 0.157

4.083 ± 0.153

5.175 ± 0.387

3.698 ± 0.202

5.742 ± 0.361

6.113 ± 0.184

6.118 ± 0.275

7.161 ± 0.225

1.707 ± 0.125

2.968 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski